描述
DO Supplement -Ade-His-Leu-Trp-Met
产品标签
YPD Medium（YEPD Medium）；YPDA Medium；YNB； Minimal SD Base；Dropout (DO) Supplements 省却成分添加剂；
产品信息
| 产品名称 | 产品编号 | 规格 | 价格（元） | 促销价（元） |
| DO Supplement -Ade-His-Leu-Trp-Met | MS6346-10G | 10g | 418 | 318 |
| DO Supplement -Ade-His-Leu-Trp-Met | MS6346-100G | 100g | 2518 | 1998 |
产品描述
酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展，这些菌株在缺乏某种特定培养基组分（比如氨基酸，嘌呤或嘧啶）的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长，正因如此，省却成分培养基添加剂（DO medium supplements）能用来筛选转化的酵母培养物。
本品为缺乏腺嘌呤（Ade）/组氨酸（His）/亮氨酸（Leu）/色氨酸（Trp）/蛋氨酸（Met）的省却成分培养基添加剂，与Minimal SD base联合使用，配制成五组分缺陷的酵母菌选择性培养基，俗称五缺培养基。用于报告基因分析、表型分析，酵母三杂交系统报告基因检测（His3, Ade2）、酵母营养缺陷型分离鉴定和表型分析。
保存与运输方法
保存：室温干燥保存，至少2年稳定。
运输：室温运输。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品
| 货号 | 名称 | 规格 | 价格（元） |
| MS6331-10G | DO Supplement -His/-Leu/-Trp | 10g | / |
| MS6332-10G | DO Supplement -His/-Leu/-Ura | 10g | / |
| MS6333-10G | DO Supplement -His/-Trp/-Ura | 10g | / |
| MS6334-10G | DO Supplement -Leu/-Met/-Trp | 10g | / |
| MS6335-10G | DO Supplement -Leu/-Trp/-Ura | 10g | / |
表1 酵母培养基配制浓度表
| 培养基类型 | 名称 | 货号 | 终浓度（g/L） |
| Rich Media
富集培养基 |
YPD Agar Medium | MS6301-350G | 70 |
| YPD Medium | MS6302-250G | 50 | |
| YPDA Agar Medium | MS6303-350G | 70 | |
| YPDA Medium | MS6304-250G | 50 | |
| Minimal Media
基本培养基 |
Minimal SD Agar Base | MS6311-467G | 46.7 |
| Minimal SD Base | MS6312-267G | 26.7 | |
| Minimal SD Agar Base Gal/Raf | MS6313-270G | 54 | |
| Minimal SD Base Gal/Raf | MS6314-185G | 37 | |
| Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | MS6316-10G | 1.29 |
| DO Supplement -Leu | MS6317-10G | 1.29 | |
| DO Supplement -Trp | MS6318-10G | 1.29 | |
| DO Supplement -Ura | MS6319-10G | 1.29 | |
| Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | MS6320-10G | 1.19 |
| DO Supplement -His/-Ura | MS6321-10G | 1.27 | |
| DO Supplement -Leu/-Trp | MS6322-10G | 1.19 | |
| DO Supplement -Met/-Trp | MS6323-10G | 1.27 | |
| DO Supplement -Trp/-Ura | MS6324-10G | 1.27 | |
| DO Supplement -Trp/-His | MS6325-10G | 1.27 | |
| DO Supplement -Ura/-Leu | MS6326-10G | 1.27 | |
| Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | MS6331-10G | 1.17 |
| DO Supplement -His/-Leu/-Ura | MS6332-10G | 1.17 | |
| DO Supplement -His/-Trp/-Ura | MS6333-10G | 1.25 | |
| DO Supplement -Leu/-Met/-Trp | MS6334-10G | 1.17 | |
| DO Supplement -Leu/-Trp/-Ura | MS6335-10G | 1.17 | |
| Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | MS6341-10G | 1.15 |
| DO Supplement -His/-Leu/-Met/-Trp | MS6342-10G | 1.15 | |
| DO Supplement -His/-Leu/-Trp/-Ura | MS6343-10G | 1.15 |
— — Written/Edited by V. Shallan【版权归金畔生物/MKBio金畔所有】