标签归档：conj

L-磷脂酰乙醇胺 PEG N-羟基琥珀酰亚胺

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基本信息

产品名称 L-磷脂酰乙醇胺 PEG N-羟基琥珀酰亚胺
英文名称 DPPE PEG NHS
规格或纯度 MW 3400 Da
运输条件 超低温冰袋运输

一般描述

1,2-Dipalmitoryl-sn-Glycero-3-Phosphoethanolamine (DPPE) conjμgated Polyethylene Glycol, DPPE PEG is a phospholipid PEG conjμgate which has both hydrophilicity and hydrophobility. Pegylated phospholipids are excellent liposome formation materials that can be used for drμg delivery, gene transfection and vaccine delivery as well. Pegylation of phospholipids significantly improves the blood circulation time and stability for encapsulated drμgs. These materials can also be used for targeted drμg delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

1,2-Dipalmitoryl-sn-Glycero-3-Phosphoethanolamine (DPPE) conjugated Polyethylene Glycol, DPPE PEG is a phospholipid PEG conjugate which has both hydrophilicity and hydrophobility. Pegylated phospholipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. Pegylation of phospholipids significantly improves the blood circulation time and stability for encapsulated drugs. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

相关属性

溶解性 Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
储存温度 -20°C储存,充氩
品牌 金畔生物

L-磷脂酰乙醇胺 PEG N-羟基琥珀酰亚胺

发表于由

基本信息

产品名称 L-磷脂酰乙醇胺 PEG N-羟基琥珀酰亚胺
英文名称 DPPE PEG NHS
规格或纯度 MW 5000 Da
运输条件 常规运输

一般描述

1,2-Dipalmitoryl-sn-Glycero-3-Phosphoethanolamine (DPPE) conjμgated Polyethylene Glycol, DPPE PEG is a phospholipid PEG conjμgate which has both hydrophilicity and hydrophobility. Pegylated phospholipids are excellent liposome formation materials that can be used for drμg delivery, gene transfection and vaccine delivery as well. Pegylation of phospholipids significantly improves the blood circulation time and stability for encapsulated drμgs. These materials can also be used for targeted drμg delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

1,2-Dipalmitoryl-sn-Glycero-3-Phosphoethanolamine (DPPE) conjugated Polyethylene Glycol, DPPE PEG is a phospholipid PEG conjugate which has both hydrophilicity and hydrophobility. Pegylated phospholipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. Pegylation of phospholipids significantly improves the blood circulation time and stability for encapsulated drugs. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

相关属性

溶解性 Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
品牌 金畔生物
备注 卖完停产，不再备货

甲氧基 PEG 氨基丁二酸 N-羟基琥珀酰亚胺

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基本信息

产品名称 甲氧基 PEG 氨基丁二酸 N-羟基琥珀酰亚胺
英文名称 Methoxy PEG amido succinic acid NHS
规格或纯度 MW 5000 Da
运输条件 超低温冰袋运输
生化机理

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjμgation.

Materials Required:

  • Conjμgation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
  • PEG NHS stock solution: 100 mg in 1 mL conjμgation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjμgation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjμgation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjμgation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjμgates can be purified either by size exclusion chromatography or dialysis.

一般描述

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

相关属性

溶解性 Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
储存温度 -20°C储存
品牌 金畔生物

NHS-PEG-NHS

发表于由

基本信息

产品名称 NHS-PEG-NHS
英文名称 NHS-PEG-NHS
别名 N-羟基琥珀酰亚胺-PEG-N-羟基琥珀酰亚胺
英文别名 Bis-succinimidyl PEG Ester
规格或纯度 MW 6000 Da
运输条件 超低温冰袋运输
生化机理

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjμgation.

Materials Required:

  • Conjμgation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
  • PEG NHS stock solution: 100 mg in 1 mL conjμgation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjμgation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjμgation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjμgation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjμgates can be purified either by size exclusion chromatography or dialysis.

一般描述

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

相关属性

敏感性 对湿度敏感
溶解性 Soluble in regular aqeous solution as well as most organic solvents
储存温度 -20°C储存,充氩
品牌 金畔生物