DO Supplement -Trp/-His

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描述

DO Supplement -Trp/-His

产品标签

YPD Medium（YEPD Medium）；YPDA Medium；YNB；Minimal SD Base；Dropout (DO) Supplements 省却成分添加剂；

产品信息

 

  产品名称                                 产品编号             规格              价格（元）     促销价（元）  
  DO Supplement -Trp/-His   MS6325-10G   10g   418   318
  DO Supplement -Trp/-His   MS6325-100G   100g   2518   1998

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展，这些菌株在缺乏某种特定培养基组分（比如氨基酸，嘌呤或嘧啶）的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长，正因如此，省却成分培养基添加剂（DO medium supplements）能用来筛选转化的酵母培养物。

本品为缺乏色氨酸（Trp）/组氨酸（His）的省却成分培养基添加剂，与Minimal SD base联合使用，配制成双组分缺陷的酵母菌选择性培养基，俗称双缺培养基（二缺培养基），用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存：室温干燥保存，至少2年稳定。

运输：室温运输。

注意事项

为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                         规格               价格（元）  
MS6320-10G DO Supplement -His/-Leu 10g 418
MS6321-10G DO Supplement -His/-Ura 10g 418
MS6322-10G DO Supplement -Leu/-Trp 10g 418
MS6323-10G DO Supplement -Met/-Trp 10g 418
MS6324-10G DO Supplement -Trp/-Ura 10g 418
MS6325-10G DO Supplement -Trp/-His 10g 418
MS6326-10G DO Supplement -Ura/-Leu 10g 418

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度（g/L）     
Rich Media

富集培养基

 YPD Agar Medium MS6301-350G 70
YPD Medium MS6302-250G 50
YPDA Agar Medium MS6303-350G 70
YPDA Medium MS6304-250G 50
Minimal Media

基本培养基

 Minimal SD Agar Base MS6311-467G 46.7
Minimal SD Base MS6312-267G 26.7
Minimal SD Agar Base Gal/Raf MS6313-270G 54
Minimal SD Base Gal/Raf MS6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

 DO Supplement -His MS6316-10G 1.29
DO Supplement -Leu MS6317-10G 1.29
DO Supplement -Trp MS6318-10G 1.29
DO Supplement -Ura MS6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

 DO Supplement -His/-Leu MS6320-10G 1.19
DO Supplement -His/-Ura MS6321-10G 1.27
DO Supplement -Leu/-Trp MS6322-10G 1.19
DO Supplement -Met/-Trp MS6323-10G 1.27
DO Supplement -Trp/-Ura MS6324-10G 1.27
DO Supplement -Trp/-His MS6325-10G 1.27
DO Supplement -Ura/-Leu MS6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

 DO Supplement -His/-Leu/-Trp MS6331-10G 1.17
DO Supplement -His/-Leu/-Ura MS6332-10G 1.17
DO Supplement -His/-Trp/-Ura MS6333-10G 1.25
DO Supplement -Leu/-Met/-Trp MS6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura MS6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

 DO Supplement -Ade/-His/-Leu/-Trp MS6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp MS6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura MS6343-10G 1.15