Fluorescent Brightener 28 (Calcofluor White M2R) 荧光增白剂28


描述

Fluorescent Brightener 28 荧光增白剂28

产品标签

Fluorescent Brightener 28 荧光增白剂28;Calcofluor White M2R;Fungi 真菌;Chitin 几丁质;CAS NO. 4404-43-7;

 

产品信息

产品名称 产品编号           CAS NO.        规格           价格(元) 
Fluorescent Brightener 28 荧光增白剂28     MS4040-1G 4404-43-7 1g 578
Fluorescent Brightener 28 荧光增白剂28 MS4040-5G 4404-43-7 5g 2318

产品描述

荧光增白剂28(Fluorescent Brightener 28),也称为Calcofluor White M2R,常用作真菌染色和细胞活力染色。该染料的用途广泛,包括:1)作为荧光增白剂,工业上用在纤维素和锦纶织物、纸张,和去污剂,肥皂;2)鉴定和研究几丁质的结构和生物合成。几丁质是自然界中发现第二丰富的多糖,来源于淡水海绵Spongilla lacustris。当与几丁质结合后,荧光增白剂28的荧光得以加强,可用来阐述骨架结构中几丁质的特殊位置;3)用来染色真菌细胞壁;4)用来染色白色念珠菌(Candida albicans)生物膜。

本品以粉末形式提供,CAS NO.4404-43-7,典型工作浓度为0.05 – 0.20%。

作用机制

荧光增白剂28(Fluorescent Brightener 28),同义名Calcofluor White M2R,非特异性结合含β-1,3-连接或β-1,4-连接的多糖,比如几丁质(chitin),β-葡聚糖和纤维素,因此能够用来快速检测细胞壁含此类成分的酵母菌、真菌和寄生生物等。其最大的吸收波长为365nm(UV,紫外激发),最大发射波长为450nm。

产品应用(来自文献):

1)Gerphagnon M et al. A Double Staining Method Using SYTOX Green and Calcofluor White for Studying Fungal Parasites of Phytoplankton. Appl Environ Microbiol. 2013 Jul;79(13):3943-51. PMID: 23603679 [CFW was used at a 2.5% (vol/vol) final concentration,zoosporic content of sporangia,Fungi]

2)Monheit JG et al. Calcofluor white detection of fungi in cytopathology. Am J Clin Pathol. 1986 Feb;85(2):222-5. [CFW was used at a 0.1% aqueous solution,Fungi in cytology specimens]

3)Domozych D et al. Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum. Methods Mol Biol. 2017; 1563:91-105. PMID: 28324604 [Unicellular Model Plant, Penium margaritaceum]

4)Hervé C et al. Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls. Methods Mol Biol. 2011; 715:103-13. PMID: 21222079 [0.25% (w/v) Calcofluor White was used, Plant Cell]

5)Demarco D et al. Histochemical Analysis of Plant Secretory Structures.Methods Mol Biol. 2017;1560:313-330. [Calcofluor white solution: dissolve 0.01 g calcofluor white MR2 in 100 mL of distilled water. Store at 4 °C. Plant Cell]

6)Arcones I et al. Monitoring Chitin Deposition During Septum Assembly in Budding Yeast.Methods Mol Biol. 2016;1369:59-72. PMID: 26519305

7)Lee KJ et al. Resin Embedding, Sectioning, and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues. Methods Mol Biol. 2014; 1080:41-52. PMID: 24132417 [ Calcofluor White: 0.25 % (w/v) in PBS]

8)Green LC et al. Discrimination between Viable and Dead Encephalitozoon cuniculi (Microsporidian) Spores by Dual Staining with Sytox Green and Calcofluor White M2R. J Clin Microbiol. 2000 Oct;38(10):3811-4. PMID: 11015407 [The slides were allowed to dry, quickly fixed in methanol, stained with 5 mg of Calcofluor White M2R/ml of H2O for 5 min, rinsed in H2O, and allowed to dry. Encephalitozoon cuniculi spores]

9)Stagoj MN et al. Microtiter plate assay of yeast cell number using the fluorescent dye Calcofluor White M2R. Biotechniques. 2004 Mar;36(3):380-2. PMID: 15038150 [Calcofluor White M2R stock solution was prepared by dissolving 48 mg solid dye in 10 mL water and filter sterilized. Stock solution can be stored at -20°C for up to 6 months. Calcofluor staining solution in buffer A (25 μM) was made fresh daily and maintained at room temperature in the dark. Cultivate yeast in the microtiter plate.]

 

产品特性

1)CAS NO:4404-43-7

2)同义名:Calcofluor White M2R卡尔科弗卢尔白M2R;Calcofluor M2R;Tinopal UNPA-GX;

3)分子式:C40H44N12O10S2

4)分子量:916.98g/mol

5)外观:黄色至米黄色粉末

6)Ex/Em:365/435nm

7)溶解性:溶于水(1mg/ml),无水乙醇(2mg/ml),DMSO(50mg/ml),微溶于甲醇

8)化学结构式:

 

保存与运输方法

保存:室温干燥保存,3年有效。

运输:室温运输。

常见问题(FAQs)

一、如何配制储存液?

Calcofluor White M2R能溶于DMSO(50mg/ml),也能溶于水(1mg/ml),但溶于水可能需借助50% NaOH或KOH来促进完全溶解。

参考方法:称取100mg粉末加入90ml去离子水(蒸馏水),边搅拌边逐滴加入20%(w/v) KOH,直至溶液变得透明。然后用水定容到100ml即得到1mg/ml储存液,0.22um滤膜过滤除菌(可选),按照单次用量分装,-20℃分装冻存。工作液浓度是储存液的10%(v/v)。具体的储存液配制浓度和溶剂请根据实际实验需求来调整。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

 

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