描述
BMPO (Spin trapping reagent)自旋捕获剂
产品标签
BMPO；DEPMPO；EMPO；Spin trapping reagent自旋捕获剂；电子顺磁共振波谱（EPR）；CAS: 387334-31-8；
产品信息
|
产品名称 |
产品编号 | CAS NO. | 规格 | 价格（元） |
|
BMPO (Spin trapping reagent)自旋捕获剂 |
MX4722-5MG | 387334-31-8 | 5mg | 1646 |
| BMPO (Spin trapping reagent)自旋捕获剂 | MX4722-10MG | 387334-31-8 | 10mg |
2486 |
| BMPO (Spin trapping reagent)自旋捕获剂 | MX4722-50MG | 387334-31-8 | 50mg |
8236 |
产品描述
BMPO（5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide）是一种新型高效且高稳定型的硝酮自旋捕获剂（Nitrone Spin Trapping Reagent），由美国威斯康星医学院Kalyanaraman教授的实验室团队开发，非常适合用于特异性检测和鉴定体内外形成的硫自由基、羟基自由基（•OH）和超氧阴离子自由基（•O2-），通过形成用电子顺磁共振波谱（EPR）可区分的加合物来测定[1-2]。
BMPO具有以下特点：
①特异性高，半衰期长：其它的硝酮自旋捕获剂比如：DMPO，难以轻易区分超氧阴离子和羟基自由基，因产生的DMPO-超氧加合物（半衰期t½=45s）瞬间衰减产生DMPO-羟基加合物。与最近开发的自旋捕获剂：DEPMPO和EMPO类似，BMPO-超氧加合物不会衰减生成羟基加合物，但是，BMPO-超氧加合物的半衰期最长（t½=23min）。
②产品稳定性高：DEPMPO和EMPO是液体自旋捕获剂，通常被硝基氧杂质污染，且效期有限。BMPO是固体的环形硝酮，通过结晶以高度纯化的状态提供，能够保存更长的周期，且不用担心降解。
③更高的信噪比：BMPO衍生的加合物在各自的EPR光谱中具有更高的信噪比，使其更适合用于检测细胞悬浮液中的亚硫酸、羟基和甲基自由基。
④高水溶性：水溶性好，更利于水相体系自由基的研究，尤其是生物体系的自由基研究。
产品特性
1） CAS NO：387334-31-8
2） 化学名：3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide
3） 同义名：5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; BocMPO;
4） 分子式：C10H17NO3
5） 分子量：199.2
6） 纯度：≥98%
7） 外观：结晶或结晶性粉末
8） 溶解性：溶于水、PBS（pH 7.2, 10mg/ml）、DMSO（25mg/ml）、无水乙醇（25mg/ml）
8）化学结构式：
保存与运输方法
保存：-20ºC干燥保存，2年有效。
运输：冰袋运输。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
参考文献
1. Zhao, H., Joseph, J., Zhang, H., et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: A relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic. Biol. Med. 31(5), 599-606 (2001).
2. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).
应用示例（仅作参考）
1.文献来源：Mitchell DG, Rosen GM, Tseitlin M, Symmes B, Eaton SS, Eaton GR. Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals. Biophys J. 2013 Jul 16;105(2):338-42. doi: 10.1016/j.bpj.2013.06.005. PMID: 23870255; PMCID: PMC3714875. Editedby MKBIO
①黄嘌呤-黄嘌呤氧化酶体系内超氧阴离子（O2⋅−）生成的测定：Typically, xanthine oxidase (0.04 U/mL) was added to pH ∼7.4 sodium phosphate buffer (50 mM) containing DTPA (1 mM) and hypoxanthine (0.5–400 μM, final concentration) to achieve rates of O2⋅− formation that ranged from 0.1 to 6.0 μM/min. We estimated the superoxide formation rate by monitoring the SOD-inhibitable reduction of ferricytochrome c (80 μM) at room temperature. Spin trapping was performed by addition of 100 mM BMPO in pH ∼7.4 phosphate-buffered saline (PBS; 50 mM) containing 1 mM DTPA to the solution of hypoxanthine and xanthine oxidase to achieve a final BMPO concentration of 50 mM in the reaction mixture. EPR spectra were recorded 10 min after mixing reagents. The half-life of BMPO-OOH at ambient temperature is reported to be ∼23 min. Solutions for control experiments contained SOD (30 U/mL).
Figure 2 Comparison of CW and rapid-scan spectra of BMPO-OOH in solution with a O2⋅− production rate of 0.1μM/min, recorded 10 min after mixing reagents. The O2⋅− was produced by a hypoxanthine/xanthine oxidase mixture. The concentration of BMPO-OOH is ∼0.3μM. (A) CW spectrum obtained with 55 G sweep width, 0.75 G modulation amplitude, single 30 s scan, 15 ms conversion time, 10 ms time constant, and 20 mW (B1 = 170 mG) microwave power. (B) Deconvolved rapid-scan spectrum obtained with 55 G scan width, 51 kHz scan frequency, and 53 mW (B1 = 250 mG) microwave power. Segments consisting of 12 sinusoidal cycles were averaged 100 k times, with a total data acquisition time of ∼30 s.
2.文献来源：Wang, Z., Zhang, Y., Ju, E.et al. Biomimetic nanoflowers by self-assembly of nanozymes to induce intracellular oxidative damage against hypoxic tumors.Nat Commun 9,3334 (2018). https://doi.org/10.1038/s41467-018-05798-x
①缺氧下的ESR测定（O2•−，•OH）：For O2•− detection, the pre-deoxidized PBS (pH 5.0, 25 mM) contained 25 mM BMPO, 20 μg mL−1 MnO2@PtCo nanoflowers, 100 μM H2O2, 50% DMSO was prepared. After incubation of 5 min, ESR spectra were recorded. For •OH detection, the pre-deoxidized PBS (pH 5.0, 25 mM) contained 25 mM BMPO, 20 μg mL−1 MnO2@PtCo nanoflowers, 100 μM H2O2, 0.25 U mL−1 SOD was prepared. After incubation of 5 min, ESR spectra were recorded. The following instrument settings were used for collecting ESR spectra: 1 G field modulation, 100 G scan range, and 20 mW microwave power.
Fig f ESR spectra of BMPO/•OOH adducts from different groups in the hypoxic H2O2 (100 μM) condition upon addition of DMSO.g ESR spectra of BMPO/•OH adducts were collected from different samples in the hypoxic H2O2 (100 μM) condition upon addition of SOD. Data were presented as mean ± s.d. (n= 5).
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