Ac-DEVD-CHO (Caspase-3/7 inhibitor) 凋亡相关蛋白Caspase-3/7抑制剂


描述

Ac-DEVD-CHO (Caspase-3/7 inhibitor)

凋亡相关蛋白Caspase-3/7抑制剂

产品标签

Ac-DEVD-CHO;Z-DEVD-FMK;Z-VAD-FMK;Caspase-3 inhibitor;细胞凋亡抑制剂;CCCP;CAS: 184179-08-6;CAS:169332-60-9;

产品信息

产品名称 产品编号 规格 价格(元)
Ac-DEVD-CHO (Caspase-3/7 inhibitor)凋亡相关蛋白Caspase-3/7抑制剂 MX3273-1MG 1mg 1280
MX3273-5MG 5mg 3680

产品描述

Ac-DEVD-CHO是一种合成四肽,是II类caspases-caspase-3/7的有效抑制剂,Kis分别是0.23和1.6nM。常用来抑制凋亡细胞内的caspase-3/7活性,以及研究caspase-3/7活化后的下游事件。另外,Ac-DEVD-CHO还能抑制其他caspases,对caspases-1/-2/-4/-5/-6/-8/-9和-10的Kis分别是18、1710、132、205、31、0.92、60和12 nM[1]。

产品特性

1) CAS NO.:169332-60-9

2) 化学名:N-acetyl-L-α-aspartyl-L-α-glutamyl-N-(2-carboxyl-1-formylethyl)-L-valinamide

3) 同义名:N-Ac-Asp-Glu-Val-Asp-CHO; Caspase-3 Inhibitor I;

4) 序列:N-Acetyl-Asp-Glu-Val-Asp-al

5) 分子式:C20H30N4O11

6) 分子量:502.47 g/mol

7) 纯度:≥98%

8) 溶解性:溶于DMSO(~25mg/ml)、乙醇(~30mg/ml)、H2O(10mg/ml)

保存与运输方法

保存:-20℃干燥保存,至少1年稳定。

运输:冰袋运输。

注意事项

1)第一次溶解粉末配制储存液时,请根据单次使用次数分装冻存,一定要避免反复冻融,以保证产品的活性不受影响。

2)-20℃冻存的储存液从冰箱取出后,可在25℃水浴锅温育片刻直到全部融解,离心后待用。

3)本品仅用作科研用途,不得用作临床诊断或治疗,不得用于食品或药品,绝对禁止用在人身上。

4)为了您的安全和健康,请穿实验服并戴一次性手套操作。

储存液配制

质量

溶剂体积

浓度

1mg 5mg 10mg
1mM 1.9902 mL 9.9508 mL 19.9017 mL
5mM 0.3980 mL 1.9902 mL 3.9803 mL
10mM 0.1990 mL 0.9951 mL 1.9902 mL

使用方法【源自文献,仅作参考】

文献1,He LG et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2014 Feb;35(2):203-10.PMID: 24362325

体外研究(细胞实验):

细胞类型(Cell type):Osteoclast-like cells (OCLs)

实验方法(Assay):OCLs were incubated with RANKL followed by different concentrations of SIN (0.5 and 1 mmol/L) or camptothecin (4 μmol/L) with or without Ac-DEVD-CHO (10 μmol/L)for 24 h. Camptothecin was used as a positive control to induce apoptosis. Caspase-3 activity in the cells was measured using the caspase-3 colorimetric activity assay kit.

文献2,Katai N et al. Apoptotic retinal neuronal death by ischemia-reperfusion is executed by two distinct caspase family proteases. Invest Ophthalmol Vis Sci. 1999 Oct;40(11):2697-705. PMID: 10509668

体内研究(动物模型):

动物模型(Animal Model):Ischemia Model of Male Sprague–Dawley rats weighing 250 to 300 g

实验方法(Assay):A 20mM solution of Ac-YVAD-CHO and Ac-DEVD-CHO in DMSO was prepared, and 2 μl of the solution was injected into the vitreous cavity with a 30-gauge needle 24 hours before ischemic insult.Another 3 μl was injected into the vitreous at the time of reperfusion. As a control, DMSO (vehicle) was injected in the same way. Twenty-four hours after reperfusion, eyes were enucleated, and TUNEL staining was performed.

相关产品

货号 名称 规格
MX3270-1MG Pan-caspase Inhibitor Z-VAD-FMK 1mg
MX3271-1MG Z-YVAD-FMK (Caspase-1 inhibitor) 1mg
MX3272-1MG Z-DEVD-FMK (Caspase-3 inhibitor) 1mg
MX3273-1MG Ac-DEVD-CHO (Caspase-3/7 inhibitor) 1mg
MX3258-100UL CCCP (50 mM)凋亡诱导剂/质子载体 100μl
MX3259-100UL FCCP (50 mM)凋亡诱导剂/质子载体 100μl
MX3210-20T Annexin V-FITC/PI Apoptosis Detection Kit细胞凋亡检测试剂盒 20T
MX3211-20T Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit细胞凋亡检测试剂盒 20T