Forskolin (50mM in DMSO) 佛司可林


描述

Forskolin 佛司可林/毛喉素

产品标签

Forskolin (Fsk) 佛司可林;Adenylate cyclase (AC) 腺苷酸环化酶;cAMP 环磷酸腺苷;Neuron Differentiation 神经元分化;CAS NO:66575-29-9;

产品信息

产品名称 产品编号                规格                CAS NO.            价格(元)    
Forskolin (50mM in DMSO) 佛司可林     MZ2301-50UL 50μl 66575-29-9 150
Forskolin (50mM in DMSO) 佛司可林 MZ2301-250UL 5×50μl 66575-29-9 520
Forskolin (Powder) 佛司可林 MZ2301-50MG 50mg 66575-29-9 1480
Forskolin (Powder) 佛司可林 MZ2301-100MG 100mg 66575-29-9 2540

产品描述

佛司可林(Forskolin,CAS NO.:66575-29-9)是一种来源于印度彩叶植物毛喉鞘蕊花(Coleus forskohlii)的天然生成二萜。通过作用于催化亚基直接激活腺苷酸环化酶(AC),引起细胞内cAMP水平的提高[1-2]。在稳定转染且表达I型腺苷酸环化酶(AC1)的Sf9细胞,Forskolin与膜上的AC1结合(IC50=41nM)并活化其功能(EC50=0.5 μM,评估从ATP生成cAMP的激活实验)[3]。Forskolin以浓度依赖性的方式发挥平滑肌松弛剂和血管扩张剂的功能,同时影响细胞质钙离子水平和收缩成分的钙离子敏感性[4]。Forskolin能够与葡萄糖转运体和某些离子通道相互作用,可用来检测腺苷酸环化酶的表达、调控和G蛋白信号通路[5]。Forskolin用于几种分化方案中以增强神经元分化能力[6-7]。

我司提供两种形式的佛司可林(Forskolin):

一种是溶于DMSO的50mM储存液形式,对应货号是:MZ2301-50UL(~1mg),MZ2301-250UL(~5mg),直接用培养基稀释到所需工作浓度即可。操作简单方便。

一种是冻干粉形式,对应货号是:MZ2301-50MG,MZ2301-100MG。需要先用DMSO或无水乙醇配制成储存液,再用培养基稀释到所需工作浓度使用。

Forskolin通常用在各种类型的完整细胞或组织培养物中来提高cAMP水平,常用工作浓度为10-50μM。具体的最佳工作浓度请参考文献,或根据自身实验体系,培养细胞和组织类型,通过实验进行摸索和优化。

产品特性

1)CAS NO:66575-29-9

2)化学名:5-(acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-(3R,4aR,5S,6S, 6aS,10S,10aR,10bS)-1H-naphtho[2,1-b]pyran-1-one

3)同义名:Coleonol洋紫苏醇;NSC 375489;NSC 357088;L 75-1362B;HL 362;

4)溶解性:溶于乙醇(~15 mg/ml),DMSO(~30 mg/ml),DMF(~30 mg/ml),几乎不溶于水

5)分子式:C22H34O7

6)分子量:410.50 g/mol

7)纯度:>99%

8)外观:白色结晶性粉末

9)化学结构式:

保存与运输方法

保存:冻干粉-20ºC避光保存,至少3年有效;DMSO储存液-20ºC避光保存至少6个月有效;

运输:冰袋运输。

使用方法【源自文献,仅作参考】

文献1,Rodriguez G et al.Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex. J Biol Chem. 2013 Mar 8;288(10):7137-46.PMID: 23341462

体外研究:

细胞类型(Cell type):Human leukemic cell line MT-2 cells

药物配制(Preparation):Forskolinwas dissolved in DMSO to make stock solution.

实验方法(Assay):MT-2 cells were seeded into 96-well plates at 5 × 104 cells per well. Cells were then pretreated for1 h with 1% DMSO (vehicle) or Fsk at 1, 5, 10, 25, 50, and 100 μM concentrations. The cells were stimulated with IL-2 as above and cultured for an additional 20 h at 37 °C. Control cells were treated with 1% DMSO for 20 h. During the final 4 h of incubation, the cells were pulsed with [3H]thymidine at a concentration of 0.5 μCi/200 μl. Cells were harvested onto fiberglass filters and analyzed using liquid scintillation counting.Fsk treatment inhibited the proliferation of MT-2 cells in a dose-dependent manner with an IC50 equal to∼5 μM Fsk. 

文献2,Matsumiya W et al. Forskolin modifies retinal vascular development in Mrp4-knockout mice. Invest Ophthalmol Vis Sci. 2012 Dec 7;53(13):8029-35. PMID:23154460

体内研究:

动物模型(Animal Model):C57BL/6J mice and Mrp4-knockout mice

药物配制(Preparation):Forskolin was dissolved in dimethyl sulfoxide (DMSO) and then diluted.

注射剂量(Dosages):Forskolin was dissolved in DMSO and injected intraperitoneally into neonatal mice at postnatal days 4 (P4) (1.0 μg/50 μL (0.3 mg/kg))and 5 (P5)(1.5 μg/50 μL (0.5 mg/kg)). Mice injected with DMSO served as the controls. The treated mice were euthanized at P6, and their retinas were isolated for whole-mount immunohistochemistry (IHC)

给药途径(Administration):Intraperitoneally (i.p.) Injection

注意事项

1)本品不是临床药物,只能用于科研用途,不能用于诊断或临床用途。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

参考文献

[1] Barber, R. and Goka, T.J. “Adenylate cyclase activity as a function of forskolin concentration.” J. Cyclic Nucleotide Protein Phosphor. Res. 10: 23-29 (1985).

[2] Laurenza, A., et al. “Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action?” Trends Pharm. Sci. 10: 442-447 (1989).

[3] Robbins JD et al. Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem. 1996 Jul 5;39(14):2745-52.

[4] Abe, A. and Karaki, H.J. “Effect of forskolin on cytosolic Ca++ level and contraction in vascular smooth muscle.” J. Pharmacol. Exp. Ther. 249: 895-900 (1989).

[5] Insel PA et al. Forskolin as a tool for examining adenylyl cyclase expression, regulation, and G protein signaling. Cell Mol Neurobiol. 2003 Jun;23(3):305-14.

[6] Palmer TD et al. The adult rat hippocampus contains primordial neural stem cells.Mol Cell Neurosci. 1997;8(6):389-404.

[7] Son H et al. Pairing of forskolin and KCl increases differentiation of immortalized hippocampal neurons in a CREB Serine 133 phosphorylation-dependent and extracellular-regulated protein kinase-independent manner. Neurosci Lett. 2001 Jul 27;308(1):37-40.

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