品牌：Bioss/博奥森 | 货号：JP-0162R
| 产品编号 | JP-0162R |
| 英文名称 | iNOS |
| 中文名称 | 一氧化氮合成酶-2（诱导型）抗体 |
| 别 名 | i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN. |
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Specific References (47) | JP-0162R has been referenced in 47 publications.
111 [IF=2.35] Li, Guofu, et al. “The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats.” International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat. 222
111 [IF=4.56] Shanshan et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. (2017) Front.Cell.Neurosc. 11:99 WB ; Rat. 222
PubMed:28424593
111 [IF=2.24] Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95 IHC ; Rat. 222
PubMed:26191216
111 [IF=5.64] Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880 WB ; Mouse. 222
PubMed:30158589
111 [IF=2.8] Liu J et al. Alterations of antioxidant indexes and inflammatory cytokine expression aggravated hepatocellular apoptosis through mitochondrial and death receptor-dependent pathways in Gallus gallus exposed to arsenic and copper. Environ Sci Pollut Res Int WB ; Chicken. 222
PubMed:29569195
111 [IF=2.332] Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437. WB ; Mouse. 222
PubMed:30316005
111 [IF=2.655] Horváth E et al. Ischemic damage and early inflammatory infiltration are different in the core and penumbra lesions of rat brain after transient focal cerebral ischemia. J Neuroimmunol. 2018 Nov 15;324:35-42. IHC-P ; Rat. 222
PubMed:30212790
111 [IF=4.183] Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). IHC-P ; Rat. 222
PubMed:30586863
111 [IF=3.118] Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36. IHC-P ; Rat. 222
PubMed:30785088
111 [IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523. WB ; Human. 222
PubMed:31383786
111 [IF=2.914] Guo MH et al. Elemental imbalance elicited by arsenic and copper exposure lead to oxidative stress and immunotoxicity in chicken gizzard, activating the protective effects of heat shock proteins. Environmental Science and Pollution Research. 2019. WB ; Chicken. 222
111 [IF=3.87] Jiao F et al. AGK2 Alleviates Lipopolysaccharide Induced Neuroinflammation through Regulation of Mitogen-Activated Protein Kinase Phosphatase-1. J Neuroimmune Pharmacol. 2019 Nov 30. IHF ; Mouse. 222
PubMed:31786712
111 [IF=3.483] Huang P et al. Theoretical basis of nitrosomyoglobin formation in a dry sausage model by coagulase-negative staphylococci: Behavior and expression of nitric oxide synthase. Meat Sci. 2019 Nov 27;161:108022. WB ; coagulase-negative staphylococci (CNS) and P. pentosaceus. 222
PubMed:31838366
111 [IF=3.58] El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928. IHC-P ; Rat. 222
PubMed:32092384
111 [IF=3.361] Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol. 2020 Feb 9;81:106205. IHC-P ; Mouse. 222
PubMed:32050154
111 [IF=3.761] Toru Nishikawa et al. Therapeutic potential for insulin on type 1 diabetes‐associated periodontitis: analysis of experimental periodontitis in streptozotocin‐induced diabetic rats. J Diabetes Investig . 2020 Apr 17. IHC ; rat. 222
PubMed:32302049
111 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC/IF ; Rat/Mouse. 222
111 [IF=2.5] Yang F et al. The effects of binge alcohol exposure on tooth movement and associated root resorption in rats. Alcohol . 2020 Nov;88:1-9. IHC-P ; Rat. 222
PubMed:32574660
111 [IF=4.225] Jiang Q et al. Particulate Matter 2.5 Induced Developmental Cardiotoxicity in Chicken Embryo and Hatchling. Front Pharmacol. 2020 Jun 5;11:841. WB ; Chicken. 222
PubMed:32581800
111 [IF=] WB ; mouse. 222
111 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240. WB、IHC ; Mouse. 222
PubMed:33377656
111 [IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390 WB,IHC ; Mouse. 222
111 [IF=3.909] Qian Xueshen. et al. Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer’s Disease. J Alzheimers Dis. 2021 Jan;79(4):1785-1800 WB ; Mouse. 222
111 [IF=3.69] Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919 WB ; Mouse. 222
111 [IF=8.758] Yinying Sheng. et al. Development and In Vitro Biodegradation of Biomimetic Zwitterionic Phosphorylcholine Chitosan Coating on Zn1Mg Alloy. Acs Appl Mater Inter. 2020;12(49):54445–54458 IF ; Mouse. 222
111 [IF=3.368] Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4‐dinitrofluorobenzene‐Induced Atopic Dermatitis‐like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248 WB ; Mouse. 222
111 [IF=2.965] Sawada Noritaka. et al. Glucagon-Like Peptide-1 Receptor Agonist Liraglutide Ameliorates the Development of Periodontitis. J Diabetes Res. 2020;2020:8843310 IHC ; Rat. 222
111 [IF=5.88] Shuai Chen. et al. Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration. Mat Sci Eng C-Mater. 2021 Jan;118:111312 IHC ; Rabbit. 222
111 [IF=8.724] Penghui Zhang. et al. Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioact Mater. 2021 Oct;6:3097 WB ; Mouse. 222
111 [IF=3.943] Kai Kang. et al. FGF21 alleviates chronic inflammatory injury in the aging process through modulating polarization of macrophages. Int Immunopharmacol. 2021 Jul;96:107634 WB ; Mouse. 222
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| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 130kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOS-2: 1051-1144/1144 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Function: Subunit: Tissue Specificity: Similarity: SWISS: Gene ID: Database links: Entrez Gene: 4843 Human Entrez Gene: 18126 Mouse Entrez Gene: 24599 Rat Omim: 163730 Human SwissProt: P35228 Human SwissProt: P29477 Mouse SwissProt: Q06518 Rat Unigene: 709191 Human Unigene: 2893 Mouse Unigene: 10400 Rat 合成与降解（Synthesis and Degradation） |
| 产品图片 |
Sample:U251 Cell Lysate at 40 ug
Primary: Anti-iNOS(JP-0162R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 130kD Observed band size: 130kD Sample:
Lane 1: Mouse Raw264.7 tissue lysates Lane 2: Human Jurkat cell lysates Primary: Anti-iNOS (JP-0162R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 140 kDa Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (JP-0162R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (JP-0162R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(JP-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(JP-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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