Products > Optimize Reagents > Optimize – Cryoprotectants > Perfluoropolyether Cryo Oil
Perfluoropolyether Cryo Oil
Applications
- Oil based cryoprotectant
Features
- Perfluoropolyether cryo oil
- Low viscosity
- Low surface tension
- Cryoprotectant for soluble and membrane protein crystals
Description
Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.
Perfluoropolyether Cryo Oil offers low viscosity, extremely low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.
Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Perfluoropolyether to the crystallization drop before mounting the crystal. After adding the Perfluoropolyether, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Perfluoropolyether will coat the mounted crystal. Cryogenically cool the mounted crystal.
应用
油基冷冻保护剂
特征
全氟聚醚冷冻油
低粘度
低表面张力
可溶性蛋白和膜蛋白晶体的冷冻保护剂
描述
在冷冻晶体学期间用于在晶体安装在冷冻环中后置换和减少晶体上的水（母液、试剂）的量。用油涂在晶体上可以最大限度地减少晶体的蒸发，并减少空气（氧气）对晶体的暴露和缓慢扩散。
全氟聚醚低温油具有低粘度、极低的蒸气压、低表面张力、优异的化学惰性和优异的热稳定性。
将镶嵌好的晶体浸入油中以置换地表水。或在安装晶体前在结晶滴中加入少量全氟聚醚。添加全氟聚醚后，使用安装的 Cryoloop 安装晶体。从液滴中取出已安装的晶体，全氟聚醚将涂覆已安装的晶体。低温冷却安装的晶体。
Perfluoropolyether Cryo Oil
CAT NO
HR2-814
NAME
DESCRIPTION
1 mL
PRICE
$30.00
cart quote
Support Material(s)
HR2-814 Perfluoropolyether Cryo Oil User Guide
HR2-814 Perfluoropolyether Cryo Oil SDSCertificate Of Analysis
Related Item(S)
- CryoPro
References
1. Structure of the ‘open form’ of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.
2. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.