It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.

PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines^®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.

PEGRx 1 contains 48 unique reagents, 10 ml each.

PEGRx 2 contains 48 unique reagents, 10 ml each.

PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

应用
用于生物大分子的初级和次级、聚合物和 pH 值结晶筛选
特征
在汉普顿研究中心开发
PEGRx 1 的主要筛选变量是聚合物类型（16 种不同的聚合物）、聚合物分子量、pH 值和低离子强度。
PEGRx 2 主要筛选变量是聚合物类型（13 种不同的聚合物）、聚合物分子量、pH 值和二级试剂，其中包括添加剂、盐、挥发性有机物和多元醇。
PEGRx HT 将 PEGRx 1 和 PEGRx 2 组合成一个 96 Deep Well 区块
pH 范围 3.5 – 9，使用 10 种不同的缓冲系统
聚合物分子量范围 200 至 20,000
配体友好，复杂友好
描述
PEGRx 是 Hampton Research 开发的基于聚合物和 pH 值的初级和次级结晶筛网。它旨在评估低离子强度 (PEGRx 1) 至中等离子强度 (PEGRx 2) 中基于聚合物的结晶试剂和 pH 值。当基于聚合物的试剂产生命中和有趣的溶解度线索时，它还被设计用作二级或优化筛选以遵循汉普顿研究指数筛选。

PEGRx 1 是一种结晶试剂套件，旨在评估在低离子强度环境与较宽 pH 范围内的一系列不同分子量的聚合物。聚合物试剂包括聚乙二醇、聚乙二醇单甲醚和 Jeffamines®。在低离子强度配方中评估了介于 200 和 20,000 之间的分子量范围。十种不同的缓冲液用于跨越 3.5 和 9 之间的 pH 值范围。主要的筛选变量是聚合物类型、聚合物分子量、pH 值和低离子强度。

PEGRx 2 是一种结晶试剂套件，设计用于在存在添加剂、盐、挥发性有机物和多元醇的中等离子强度环境中评估一系列不同分子量的聚合物，以及较宽的 pH 值范围。聚合物试剂包括聚乙二醇和聚乙二醇单甲醚。在中等离子强度配方中评估了介于 200 和 20,000 之间的聚合物分子量范围。十种不同的缓冲液用于跨越 3.5 和 9 之间的 pH 值范围。主要筛选变量是聚合物类型、聚合物分子量、pH 值和次要试剂，包括添加剂、盐、挥发性有机物和多元醇。

PEGRx 1 包含 48 种独特的试剂，每种试剂 10 ml。

PEGRx 2 包含 48 种独特的试剂，每种试剂 10 毫升。

PEGRx HT 在单个 Deep Well 模块中包含 96 个 PEGRx 1 和 PEGRx 2 试剂。

即用型试剂经过无菌过滤和超纯 1 类水配制，使用最高纯度的盐、聚合物、有机物和缓冲液。个别试剂可通过 Hampton Research Custom Shop 购买。

 

PEGRx 1

PEGRx 2

PEGRx HT

 HR2-082 PEGRx 1 Documents HR2-082 PEGRx 1 SDS HR2-084 PEGRx 2 Documents HR2-084 PEGRx 2 SDS HR2-086 PEGRx HT Documents HR2-086 PEGRx HT SDS Change in Formulation PEGRx Screens PEGRx Formulation & Scoring Data

1. Crystallization and preliminary X-ray crystallographic studies of DesR, a thermosensing response regulator in a two-component signalling system from Streptococcus pneumoniae. Ae Kyung Park, Seung Min Bong, Jin Ho Moon and Young Min Chi. Acta Cryst. (2009). F65.

2. Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors.  Zhang L, Lin D, Sun X, Curth U, Drosten C, Sauerhering L, Becker S, Rox K, Hilgenfeld R. Science. 2020 Apr 24;368(6489):409-412. doi: 10.1126/science.abb3405. Epub 2020 Mar 20.

3. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase.  Zhang, L., Xie, Z., Liu, Z. et al.  Nat Commun 11, 2676 (2020). https://doi.org/10.1038/s41467-020-16500-5.