Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256-4T
NDSB-256-4T
Applications
- Crystallization grade NDSB-256-4T for formulating screens or for optimization
Features
- Efficiently solubilizes proteins at non-denaturing concentrations
- Useful in preventing protein aggregation
- Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
- Protein specific additive for solubility and stability
- NDSB 256 4T is a non-detergent sulfobetaine (NDSB) that prevents protein aggregation and facilitates protein folding by interacting with the early folding intermediates. Reported to improve the in vitro renaturation efficiency of reduced hen egg lysozyme (60% enzymatic activity at 600 mM NDSB-256-4T) and chemically unfolded tryptophan synthase ß2 subunit (100% enzymatic activity at 1 M NDSB-256-4T).
Description
The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.
Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.
Typical useful NDSB concentration in protein sample is 0.5-1.0 M.
NDSB-256-4T
Synonym: 3-(4-tert-Butyl-1-pyridino)-1-propane sulfonate, SB256-4T, SN2564T
C12H19NO3S
CAS Number [570412-84-9]
Mr 257.35
Purity = 98% by HPLC
Hygroscopic
Store at room temperature
应用
结晶级 NDSB-256-4T 用于配制筛网或优化
特征
在非变性浓度下有效溶解蛋白质
有助于防止蛋白质聚集
非去污剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂
NDSB 256 4T 是一种非去污剂磺基甜菜碱 (NDSB)，可通过与早期折叠中间体相互作用来防止蛋白质聚集并促进蛋白质折叠。据报道，可提高降低的鸡蛋溶菌酶（600 mM NDSB-256-4T 下的酶活性为 60%）和化学未折叠色氨酸合酶 ß2 亚基（1 M NDSB-256-4T 下的酶活性为 100%）的体外复性效率。
描述
NDSB 是一组两性离子化合物，可以减少聚集并有助于重新折叠包涵体和细菌表达系统中发现的蛋白质。
非去污剂磺基甜菜碱具有磺基甜菜碱亲水基团和短疏水基团，不能聚集形成胶束，因此 NDSB 不被视为去污剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，可提高膜蛋白的产量。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。
蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。
NDSB-256-4T
同义词：3-(4-tert-Butyl-1-pyridino)-1-propane 磺酸盐，SB256-4T，SN2564T
C12H19NO3S
CAS号 [570412-84-9]
257.35 先生
纯度 = 98% HPLC
吸湿性
在室温下储存
NDSB-256-4T
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CAT NO
HR2-794
NAME
DESCRIPTION
5 grams
PRICE
$157.00
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Support Material(s)
HR2-794 NDSB-256-4T User Guide
HR2-794 NDSB-256-4T SDSCertificate Of Analysis
References
1. Expert-Bezancon, N., et. al., Biophys Chem, 100, 469 (2003).