Additive Screen • Additive Screen HT蛋白结晶-Hampton
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Products > Optimization Screens > Additive Screen > Additive Screen • Additive Screen HT
Additive Screen • Additive Screen HT
Applications
- Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility
Features
- 18 classes of reagents
- Highly concentrated (10x) reagent formulation
- 96 unique reagents and excipients
- Tube or Deep Well block format
- Crystallization or sample solubility optimization
Description
Additive Screen is a library of reagents that can affect the solubility and crystallization of biological macromolecules, including both soluble and membrane proteins.
These reagents can perturb and manipulate sample-sample and sample-solvent interactions, as well as perturb water structure which can alter and improve both the solubility and crystallization of a sample. Additives can stabilize or engender conformity by specific interaction with the macromolecules. There are numerous reports of the use of additives to improve the quality and size of macromolecular crystals.1-5
Additive screen reagent classifications include multivalent, salt, amino acid, dissociating agent, linker, polyamine, osmolyte, chaotrope, co-factor, reducing agent, chelating agent, polymer, carbohydrate, non-detergent, amphiphile, detergent, non-volatile organic and volatile organic.
Additive Screen contains 96 unique reagents, 1 ml each.
Additive Screen HT contains 96 unique reagents, 1 ml each, in a single Deep Well block.
操纵样品-样品和样品-溶剂相互作用以改善晶体或改变样品溶解度
特征
18类试剂
高浓度 (10x) 试剂配方
96 种独特的试剂和赋形剂
管或深井块格式
结晶或样品溶解度优化
描述
Additive Screen 是一个试剂库，可以影响生物大分子的溶解度和结晶，包括可溶性蛋白和膜蛋白。
这些试剂可以干扰和操纵样品-样品和样品-溶剂的相互作用，以及可以改变和改善样品溶解度和结晶的水结构。添加剂可以通过与大分子的特定相互作用来稳定或产生一致性。有许多关于使用添加剂来改善大分子晶体的质量和尺寸的报道。 1-5
添加剂筛选试剂分类包括多价、盐、氨基酸、解离剂、连接剂、多胺、渗透剂、离液剂、辅助因子、还原剂、螯合剂、聚合物、碳水化合物、非去污剂、两亲物、去污剂、非挥发性有机物和挥发性有机物。
Additive Screen 包含 96 种独特的试剂，每种试剂 1 ml。
Additive Screen HT 在单个 Deep Well 模块中包含 96 种独特的试剂，每种试剂 1 ml。
Additive Screen
CAT NO
HR2-428
NAME
DESCRIPTION
1 ml, tube format
PRICE
$454.00
cart quote
CAT NO
HR2-138
NAME
DESCRIPTION
1 ml, Deep Well block format
PRICE
$454.00
cart quote
Support Material(s)
HR2-428 Additive Screen Documents
HR2-428 Additive Screen SDS
HR2-138 Additive Screen HT Documents
HR2-138 Additive Screen HT SDS
Additive Screen Formulation & Scoring Data
Additive Screen Frequently Asked QuestionsRelated Item(S)
- Individual Additive Screen Reagents
References
1. Crystallization of membrane proteins., Edited by Hartmut Michel, CRC Press (1991).
2. Crystallization of Nucleic Acids and Proteins: A Practical Approach., Edited by A. Ducruix and R. Giege, Oxford University Press (1992).
3. Cudney, R., et al., Screening and optimization strategies for macromolecular crystal growth., Acta Cryst. (1994) D50, 414-423.
4. Sousa R., Use of glycerol, polyols and other protein structure stabilizing agents in protein crystallization., Acta Cryst. (1995) D51, 271-277.
5. Trakhanov, S. and Quiocho, F.A., Influence of divalent cations on protein crystallization., Protein Science (1995) 4, 9, 1914-1919.
6. Crystallization and preliminary X-ray diffraction analysis of YhbJ from Escherichia coli, a key protein involved in the GlmYZ sRNA regulatory cascade. M. Resch, Y. Gopel, B. Gorke and R. Ficner. M. Resch, Y. Gopel, B. Gorke and R. Ficner.
7. Crystal-contact engineering to obtain a crystal form of the Kelch domain of human Keap1 suitable for ligand-soaking experiments. Stefan Horer, Dirk Reinert, Katja Ostmann, Yvette Hoevels and Herbert Nar. Acta Cryst. (2013). F69, 592-596.
8. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Santos SP, Bandeiras TM, Pinto AF, Teixeira M, Carrondo MA, Romão CV. Protein Expr Purif. 2012 Feb;81(2):193-200. doi: 10.1016/j.pep.2011.10.001. Epub 2011 Oct 24. (Thermofluor using the Additive Screen)