Heavy Atom Screens-Hampton 重原子屏幕
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Products > Optimization Screens > Heavy Atom Screens > Heavy Atom Screens
Heavy Atom Screens
Applications
- Heavy atoms for multiple isomorphous replacement
Features
- Convenient sets of popular heavy atoms
- Each kit include a heavy atom tutorial and formulation guide
Description
A Heavy Atom Screen kit provides 50 mg of each heavy atom in an o-ring screw cap micro tube. This convenient format provides sufficient material for the preparation of numerous (15 x 100 mM or 1,500 x 1 mM) small volume (0.1 ml) fresh stock solutions of heavy atoms. The quantity is sufficient for screening and derivatization but avoids the problem of storing large quantities of of heavy atoms.
应用
多重同晶置换的重原子
特征
方便的流行重原子集
每个套件都包括重原子教程和配方指南
描述
重原子筛选套件在 O 形圈螺旋盖微管中提供 50 毫克每个重原子。 这种方便的形式为制备大量（15 x 100 mM 或 1,500 x 1 mM）小体积（0.1 ml）重原子新鲜储备溶液提供了足够的材料。 数量足够用于筛选和衍生化，但避免了储存大量重原子的问题。
Heavy Atom Screen
CAT NO
HR2-442
NAME
DESCRIPTION
50 mg, tube format
PRICE
$538.00
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CAT NO
HR2-444
NAME
DESCRIPTION
50 mg, tube format
PRICE
$369.00
cart quote
CAT NO
HR2-446
NAME
DESCRIPTION
50 mg, tube format
PRICE
$410.00
cart quote
CAT NO
HR2-448
NAME
DESCRIPTION
50 mg, tube format
PRICE
$449.00
cart quote
CAT NO
HR2-450
NAME
DESCRIPTION
50 mg, tube format
PRICE
$423.00
cart quote
Support Material(s)
HR2-442 Heavy Atom Screen Pt SDS
HR2-444 Heavy Atom Screen Au SDS
HR2-442 HR2-444 HR2-446 HR2-448 HR2-450 Heavy Atom Screens User Guide
HR2-446 Heavy Atom Screen Hg SDS
HR2-448 Heavy Atom Screen M1 SDS
HR2-450 Heavy Atom Screen M2 SDSRelated Item(S)
- Individual Heavy Atom Pt Reagents
- Individual Heavy Atom Screen Hg Reagents
- Individual Heavy Atom Screen M1 Reagents
- Individual Heavy Atom M2 Reagents
References
1. Towards a rational approach for heavy-atom derivative screening in protein crystallography. Johnson Agniswamy, M. Gordon Joyce, Carl H. Hammer, and Peter D. Sun. Acta Cryst. (2008) D64, 354-367.
2. Heavy-atom derivatization. Elspeth Garman and James W. Murray, Acta Cryst. (2003). D59, 1903-1913.
3. Screening for phasing atoms in protein crystallography. Titus J Boggon and Lawrence Shapiro. Structure 2000, Vol 8 No 7, 143-149.
4. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part II: phasing of new structures. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1099-103. Epub 2002 Jun 20. Sun PD, Radaev S.
5. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1092-8. Epub 2002 Jun 20. Sun PD, Radaev S, Kattah M.
6. Preparation of isomorphous heavy-atom derivatives. Methods Enzymol. 1985;114:147-56. Petsko GA. PMID: 4079763
7. An overview of heavy-atom derivatization of protein crystals. A. C. W. Pike, E. F. Garman, T. Krojer, F. von Delft & E. P. Carpenter (2016). Acta Cryst. D72, 303-318.
8. Fluorescence Detection of Heavy Atom Labeling (FD-HAL): a rapid method for identifying covalently modified cysteine residues by phasing atoms.Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J. J Struct Biol. 2010 Jul;171(1):82-7.
9. A rapid and rational approach to generating isomorphous heavy-atom phasing derivatives. Lu J, Sun PD. FEBS J. 2014 Sep;281(18):4021-8.
10. A rational approach to heavy-atom derivative screening. Joyce MG, Radaev S, Sun PD. Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):358-65.