I3C Phasing Kit蛋白结晶-Hampton

I3C Phasing Kit蛋白结晶-Hampton

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I3C Phasing Kit蛋白结晶-Hampton

I3C Phasing Kit蛋白结晶-Hampton

Products > Optimization Screens > I3C Phasing Kit > I3C Phasing Kit

I3C Phasing Kit

Applications

  • Produce heavy atom derivative of biological macromolecules for phasing

Features

  • I3C kit for soaking or co-crystallization

Description

I3C can be used for heavy atom derivatization of biological macromolecules for subsequent single wavelength anomalous dispersion (SAD) or single isomorphous replacement plus anomalous scattering (SIRAS).

The two carboxylic acid groups and one amino group of I3C can interact by way of hydrogen bonds with both the backbone and side chains of proteins. This can result in a relatively high occupancy of the bound ligand I3C. The three iodine atoms per I3C molecule provide for a strong anomalous signal.

With three iodine atoms per molecule it provides a strong anomalous signal, even for in-house X-ray sources, as the anomalous scattering signal f′ of iodine is 6.85 electron units at the Cu κα wavelength of 1.54 Angstrom.

The three iodine atoms in I3C form an equilateral triangle with 6.0 Å side lengths. These triangular structures can readily be identified in the anomalous electron density map.

Kit Contents
• 12 aliquots of I3C (280 mg each)
– Synonym: 5-Amino-2,4,6-triiodoisophthalic acid
– CAS Number [35453-19-1]
• 12 aliquots of 2.0 M Lithium hydroxide (650 µl each)
– CAS Number [1310-65-2]
• User Guide

应用
生产用于定相的生物大分子的重原子衍生物
特征
用于浸泡或共结晶的 I3C 套件
描述
I3C可用于生物大分子的重原子衍生化,用于后续的单波长反常色散(SAD)或单同晶置换加反常散射(SIRAS)。

I3C 的两个羧酸基团和一个氨基基团可以通过氢键与蛋白质的主链和侧链相互作用。这会导致结合的配体 I3C 的占有率相对较高。每个 I3C 分子的三个碘原子提供了强烈的异常信号。

每个分子有三个碘原子,即使对于内部 X 射线源,它也能提供强烈的异常信号,因为在 1.54 埃的 Cu κα 波长处,碘的异常散射信号 f’ 为 6.85 个电子单位。

I3C 中的三个碘原子形成一个边长为 6.0 Å 的等边三角形。这些三角形结构可以很容易地在异常电子密度图中识别出来。

套件内容
• 12 份 I3C(每份 280 毫克)
– 同义词:5-Amino-2,4,6-triiodoisophthalic acid
– CAS 编号 [35453-19-1]
• 12 份 2.0 M 氢氧化锂(每份 650 µl)
– CAS 编号 [1310-65-2]
• 用户指南

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HR3-133 I3C Phasing Kit

I3C Phasing Kit蛋白结晶-Hampton

I3C Phasing Kit蛋白结晶-Hampton

 

CAT NO

HR3-133

NAME

I3C Phasing Kit

DESCRIPTION

24 Tubes

PRICE

$195.00

cart quote

Support Material(s)

I3C Phasing Kit蛋白结晶-Hampton HR3-133 I3C Phasing Kit User GuideI3C Phasing Kit蛋白结晶-Hampton HR3-133 I3C Phasing Kit SDS

References

1. A magic triangle for experimental phasing of macromolecules. Beck et al. Acta Cryst. D64, 1179, 2008.

2. Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mhp37. Sippel et al. Acta Cryst. D64, 1172, 2008.

3. 5-Amino-2,4,6 triiodo-isophthalic acid monohydrate. Beck et al. Acta Cryst. E64:1286, 2008.