PCT™ Pre-Crystallization Test-Hampton PCT™ 预结晶测试
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Products > Crystallization Screens > PCT Pre-Crystallization Test > PCT™ Pre-Crystallization Test
PCT™ Pre-Crystallization Test
Applications
- Determine the appropriate sample concentration for crystallization screening
Features
- Conserve sample
- Optimize sample concentration prior to initial screens
- Provide insight to sample homogeneity
Description
The PCT Pre-Crystallization Test is used to determine the appropriate protein concentration for crystallization screening. Sample concentration is a significant crystallization variable. Samples too concentrated can result in amorphous precipitate, while samples too dilute can result in clear drops. Precipitate and clear drops are typical crystallization screen results for reagent conditions which do not promote crystallization and are part of every crystallization screen. However, by optimizing protein concentration for the screen, the number of clear and precipitate results can often be reduced, which in turn results in more efficient sample utilization while at the same time enhancing the chances for crystallization. PCT can minimize or prevent situations where a screen results in an over abundance of precipitate or clear drops.
The PCT kit contains 4 unique, preformulated, sterile filtered reagents used to evaluate protein concentration for crystallization screening. Initially, the sample protein is mixed with two of the reagents to determine if the protein concentration is appropriate for crystallization screening. If the protein is very sensitive to salt and polymer concentration, based on initial PCT results, the protein may be evaluated using a second set of PCT reagents. PCT results will then provide insight to either the appropriate sample concentration or indicate that other diagnostic testing such as native gel electrophoresis or dynamic light scattering should be performed to demonstrate sample homogeneity appropriate for crystallization.
应用
确定适合结晶筛选的样品浓度
特征
保存样品
在初始筛选之前优化样品浓度
深入了解样品的均匀性
描述
PCT 预结晶测试用于确定用于结晶筛选的适当蛋白质浓度。样品浓度是一个重要的结晶变量。样品太浓会导致无定形沉淀，而样品太稀会导致清晰的液滴。沉淀和清澈的液滴是不促进结晶的试剂条件的典型结晶筛选结果，并且是每个结晶筛选的一部分。然而，通过优化筛选的蛋白质浓度，通常可以减少透明和沉淀结果的数量，从而提高样品利用效率，同时增加结晶的机会。 PCT 可以最大限度地减少或防止筛网导致过多沉淀物或透明液滴的情况。
PCT 试剂盒包含 4 种独特的、预先配制的、经过无菌过滤的试剂，用于评估用于结晶筛选的蛋白质浓度。最初，样品蛋白质与两种试剂混合以确定蛋白质浓度是否适合结晶筛选。如果蛋白质对盐和聚合物浓度非常敏感，根据最初的 PCT 结果，可以使用第二组 PCT 试剂评估蛋白质。然后，PCT 结果将提供对适当样品浓度的洞察，或表明应进行其他诊断测试，例如天然凝胶电泳或动态光散射，以证明适合结晶的样品均匀性。
PCT Kit comparison table.
| Catalog Number |
4 Unique Reagents |
Glass Siliconized Cover Slides |
24 Well Plate with sealant |
| HR2-140 | ✓ (50 mL each) | ✓ (22 mm Square) | |
| HR2-141 | ✓ (10 mL each) | ||
| HR2-142 | ✓ (30 mL each) | ✓ (22 mm Square) | ✓ (5x VDX) |
| HR2-143 | ✓ (10 mL each) | ✓ (18 mm Circle) | ✓ (4x VDXm) |
PCT
CAT NO
HR2-140
NAME
DESCRIPTION
50 ml bottles (4 ea), cover slides (1 pk)
PRICE
$100.00
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CAT NO
HR2-141
NAME
DESCRIPTION
10 ml tubes (4 ea)
PRICE
$77.00
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CAT NO
HR2-142
NAME
DESCRIPTION
30 ml bottles (4 ea), cover slides (1 pk), VDX Plates with sealant (5 ea)
PRICE
$121.00
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CAT NO
HR2-143
NAME
DESCRIPTION
10 ml tubes (4 ea), cover slides (1 pk), VDXm Plates with sealant (4 ea)
PRICE
$99.00
cart quote
Support Material(s)
HR2-140 & HR2-141 & HR2-142 & HR2-143 PCT Kit Documents
HR2-140 PCT Kit (with slides) SDS
HR2-141 PCT Kit (reagents only) SDS
HR2-142 PCT Kit (with plates and slides) SDS
HR2-143 PCT Kit (with plates and slides) SDSRelated Item(S)
- Individual PCT Reagents
References
1. Crystallization and x-ray diffraction analysis of human CLEC-2. Aleksandra A. Watson and Christopher A. O’Callaghan. Acta Cryst. (2005). F61, 1094-1096.