Crystal Screen • Crystal Screen 2 • Crystal Screen HT蛋白结晶-Hampton
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Products > Crystallization Screens > Crystal Screen • Crystal Screen 2 > Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Applications
- Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules
- Sparse matrix additive screen
Features
- The original sparse matrix screen
- Sparse matrix formula efficiently samples salts, polymers, organics, & pH
- Proven effective with more than 1,000 biological macromolecules
- Tube or Deep Well block format
Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.
A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.
Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.
Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.
Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
蛋白质、可溶性肽、核酸和水溶性小分子的初步筛选
稀疏矩阵加法筛选
特征
原始稀疏矩阵屏幕
稀疏矩阵公式可有效地对盐、聚合物、有机物和 pH 值进行采样
已证明对 1,000 多种生物大分子有效
管或深井块格式
描述
Crystal Screen 和 Crystal Screen 2 试剂盒旨在为大分子的结晶提供一种高效、快速的筛选方法。这些筛选器对于寻找初始结晶条件来说既简单又实用。已使用 Crystal Screen 确定了 1,000 多种蛋白质、肽、寡核苷酸和小分子的初始结晶条件。克服对合适结晶条件的详尽搜索的一种高效方法是使用试验条件的稀疏矩阵方法，该方法有偏差并从已知的大分子结晶条件中选择。 Crystal Screen 和 Crystal Screen 2 中使用的配方评估了 96 种独特的 pH 值、盐类、聚合物和有机物混合物，以及它们促进晶体生长的能力。
Crystal Screen 包含 50 种独特的试剂，每种试剂 10 毫升，基于 Jancarik 和 Kim 于 1991 年首次描述的稀疏矩阵公式。
Crystal Screen 2 是 Crystal Screen 的扩展，包含 48 种独特的试剂，每种试剂 10 ml，基于 Cudney 等人于 1994 年首次描述的配方。
Crystal Screen HT 在单个深孔模块中包含来自 Crystal Screen 的试剂 1-48 和来自 Crystal Screen 2 的所有 48 种试剂各 1 ml。
即用型试剂经过无菌过滤和超纯 1 类水配制，使用最高纯度的盐、聚合物、有机物和缓冲液。个别试剂可通过 Hampton Research Custom Shop 购买。
Crystal Screen
CAT NO
HR2-110
NAME
DESCRIPTION
10 ml, tube format
PRICE
$253.00
cart quote
CAT NO
HR2-112
NAME
DESCRIPTION
10 ml, tube format
PRICE
$253.00
cart quote
CAT NO
HR2-130
NAME
DESCRIPTION
1 ml, Deep Well block format
PRICE
$147.00
cart quote
Support Material(s)
HR2-110 Crystal Screen Documents
HR2-110 Crystal Screen SDS HR2-112 Crystal Screen 2 Documents
HR2-112 Crystal Screen 2 SDS HR2-130 Crystal Screen HT Documents HR2-130 Crystal Screen HT SDS
Crystal Screen Formulation & Scoring DataRelated Item(S)
- Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents
References
1. Jancarik, J. & Kim, S.H. J. Appl. Cryst. 24, 409-411, (1991).
2. Cudney, R., Patel, S., Weisgraber, K., Newhouse, Y., and McPherson, A., Acta Cryst. (1994) D50, 414-423.
3. Expression, purification, crystallization and preliminary X-ray analysis of two arginine-biosynthetic enzymes from Mycobacterium tuberculosis. F. Moradian, C. Garen, L. Cherney, M. Cherney and M. N. G. James. Acta Cryst. (2006). F62, 986-988.
4. The development and application of a method to quantify the quality of cryoprotectant solutions using standard area-detector X-ray images. McFerrin and Snell, J. Appl. Cryst. (2002). 35, 538-545.
5. Get phases from arsenic anomalous scattering: de novo SAD phasing of two protein structures crystallized in cacodylate buffer. Liu X, Zhang H, Wang XJ, Li LF, Su XD. PLoS One. 2011;6(9):e24227. Epub 2011 Sep 2.
6. Cloning, crystallization and preliminary X-ray diffraction analysis of an intact DNA methyltransferase of a type I restriction-modification enzyme from Vibrio vulnificus. L. Huynh Thi Yen, S.-Y. Park and J.-S. Kim. Acta Cryst. (2014). F70, 489-492 [ doi:10.1107/S2053230X14004543 ]
7. Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis. H. Ju, R. Pandian, K. Kim, K. K. Kim and T. D. Kim. Acta Cryst. (2014). F70, 473-475 [ doi:10.1107/S2053230X14004142 ]