Harlan 他莫昔芬饮食Tamoxifen diets

发表于由

Harlan 他莫昔芬饮食Tamoxifen diets

Harlan Teklad动物饲料
欢迎新老客户访问Harlan官网或者咨询上海金畔生物获取更多详细资料。

  • Home +
  • Teklad diet, bedding and enrichment +
  • Teklad laboratory animal diets +
  • Custom research diets +
  • Tamoxifen diets

Tamoxifen diets

Tamoxifen diets

Diets with tamoxifen activate CreER

Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor. Envigo Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of doxycycline-containing diets for tet regulated systems. If you have any questions, don’t hesitate to consult a nutritionist.

Teklad stocks tamoxifen and tamoxifen citrate

Tamoxifen is added to the diet as a tamoxifen-sucrose mixture. Users are encouraged to use the stocked supply. However, Teklad will continue to work with customer prepared mixes. If you choose this option, please consult a nutritionist for additional information about preparing and sending the mixture.

含他莫昔芬的饮食可激活 CreER

一些参考文献引用了在突变雌激素受体控制下的 Cre 模型使用含他莫昔芬的饮食。 Envigo Teklad 饮食可定制各种含他莫昔芬的饮食，以满足您的研究需求。 我们还为 tet 调节系统制作各种含有强力霉素的饮食。 如果您有任何疑问，请随时咨询营养师。

Teklad 库存他莫昔芬和他莫昔芬柠檬酸盐

他莫昔芬作为他莫昔芬-蔗糖混合物添加到饮食中。 鼓励用户使用库存供应。 但是，Teklad 将继续使用客户准备的混合物。 如果您选择此选项，请咨询营养师以获取有关准备和发送混合物的更多信息。

order today

18301939375

上海金畔生物科技有限公司

Teklad offers diet with USP grade tamoxifen and tamoxifen citrate

Tamoxifen
mg per kg diet		 Daily tamoxifen
mg per kg body weight1		 Diet code (Teklad supplied tamoxifen) Diet code (Teklad supplied tamoxifen)
Natural colored Red
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400 citrate2 40 TD.130859 TD.130860 3

1 Assumes 20-25 g body weight and three-four g intake
2 Tamoxifen citrate is 66% tamoxifen
3 Referred to as TAM400/CreER in Europe (TD.55125 )

1 假设体重为 20-25 克，摄入量为 3-4 克
2 柠檬酸他莫昔芬是 66% 的他莫昔芬
3 在欧洲称为 TAM400/CreER (TD.55125 )

Please add 5-10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.

These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.

Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.

请为辐照饮食增加 5-10 天的交货时间； 库存和定制商品至少需要 3 公斤订单。

这些饮食都含有预先混合了约 5% 蔗糖的他莫昔芬作为适口性增强剂； 但是，仍然可能会出现饲料厌恶。 如果摄入量有问题，请参阅下面的建议。

Teklad Global 2016（这些示例中的基础饮食）只是 Teklad 生产的几种最低植物雌激素饮食之一。 咨询营养师关于其他基础饮食的使用。

Key planning information

  • Minimum order quantity is three kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
  • Irradiation (optional) minimal dose of 20 kGy (Must be requested at time of order)

主要规划信息

最小起订量为 3 公斤，足够喂食约 20 只老鼠一个月
将饮食冷藏并计划在六个月内使用
典型的交货时间是两周（如果受到辐照，则为四个星期）
辐照（可选）最小剂量 20 kGy（必须在订购时提出要求）

Mice need time to adjust

Plan for:

  • Initial reduction in food intake and weight loss
  • Daily observation of animal tolerance — consult your IACUC for monitoring metrics
  • Intervene when necessary

Other researchers have the following advice

If intake seems to be a problem:

  • Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
  • Gradually acclimate mice by mixing tamoxifen-containing pellets with regular feed pellets
  • Wet the food, then place in a dish inside the cage (requires daily replacement)
  • Feed tamoxifen diet on weekdays, regular diet on weekends
  • Alternate weeks (two weeks on tamoxifen diet, one week off) for longer treatments

Safe handling of tamoxifen diets

  • Tamoxifen is a selective estrogen receptor modulator (SERM) meaning it can repress actions of estrogen or have pro-estrogen effects
  • Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one to two mg/pellet
  • Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of tamoxifen containing diets.

Key points from literature

  • Length of treatment varies from one to two weeks1,2,5,6,7,8 to one to two months3,4,8
  • Pure tamoxifen1,5,8 and tamoxifen citrate1,2,3,4,6,7 are both effective
    Usual tamoxifen doses are ~40-80 mg per kg body weight per day
  • Typical inclusion for pure tamoxifen is 250 mg8 or 500 mg1,5 and for tamoxifen citrate is 400 mg per kg diet1,2,3,4,6,7
  • Initial weight loss of ten % is reported1,2,4,7,8, associated with reduced food intake2
  • Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation2,7

老鼠需要时间来调整
计划：

最初减少食物摄入和减轻体重
每日观察动物耐受性——咨询您的 IACUC 以了解监测指标
必要时进行干预
其他研究人员有以下建议
如果摄入量似乎有问题：

通过进行他莫昔芬剂量喂养试验确定较低剂量是否对您的模型有效
通过将含有他莫昔芬的颗粒与常规饲料颗粒混合来逐渐适应小鼠
弄湿食物，然后放在笼子里的盘子里（需要每天更换）
平日喂他莫昔芬饮食，周末规律饮食
隔周（他莫昔芬饮食两周，休息一周）进行更长时间的治疗
安全处理他莫昔芬饮食
他莫昔芬是一种选择性雌激素受体调节剂 (SERM)，这意味着它可以抑制雌激素的作用或具有促雌激素作用
人类治疗效果的常用剂量为 20 毫克/天。啮齿动物的他莫昔芬饮食通常含有约 1 至 2 毫克/粒
通过在处理饮食时使用实验室外套、手套和面罩等典型的实验室预防措施，可以最大限度地减少意外的他莫昔芬暴露。
应联系您的化学安全部门，以获取有关处理和处置含他莫昔芬饮食的其他机构特定指南。
文献要点
治疗时间从一到两周不等1,2,5,6,7,8 到一到两个月3,4,8
纯他莫昔芬1,5,8和他莫昔芬柠檬酸盐1,2,3,4,6,7均有效
通常的他莫昔芬剂量为每天每公斤体重约 40-80 毫克
纯他莫昔芬的典型含量为 250 mg8 或 500 mg1,5，柠檬酸他莫昔芬的含量为 400 mg/kg 饮食 1,2,3,4,6,7
据报道，最初体重减轻 10%1,2,4,7,8，这与食物摄入量减少有关 2
恢复正常饮食后体重的后续恢复可能会受到基因失活的影响2,7

Selected tamoxifen references  参考文献

1 Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010. Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts. Transgenic Res 19:715-725.

2 Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010. Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. Proc Natl Acad Sci USA 107:16320-16324.

3 Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011. Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice. PLoS One 6:e21055.

4 Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007. Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate. Genesis 45:11-16.

5 Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009. Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12-15.

6 Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. PCirc Res 106:559-572.

7 Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011. Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression. PLoS One 6:e22589.

8 Welle S, Burgess K, Thornton CA, Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.

Live chatLive chat  

Tamoxifen diets

图片仅供参考,请以实物为准。

若本网站没有及时更新，请大家谅解！
正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。