Products > Optimize Reagents > Optimize – Buffers > ADA Buffer
ADA Buffer
Applications
- Crystallization grade ADA buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
ADA (Buffer)
Synonyms: N-(2-Acetamido)iminodiacetic acid;
N-(Carbamoylmethyl)iminodiacetic acid
C6H10N2O5
H2NCOCH2N(CH2CO2H)2
Mr 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Beilstein Registry Number 1787181
Merck 14,147
Purity ≥ 99.0%
MDL Number MFCD00008031
PubChem Substance ID 24845005
pKa = 6.6 at 25°Celsius
Useful pH range of ADA is 5.6 – 7.5
Titrate HR2-507 to the desired pH using NaOH
HR2-817 is titrated to pH 6.5 using NaOH
HR2-507 measured Conductivity range: 23.6 – 28.6 mS/cm at 25°C
HR2-507 measured Refractive Index range: 1.35009 – 1.35044 at 20°C
HR2-817 measured Conductivity range: 42.2 – 50.4 mS/cm at 25°C
HR2-817 measured Refractive Index range: 1.36787 – 1.36913 at 20°C
ADA buffer is used in the Hampton Research MembFac crystallization kit.
Total Impurities Insoluble matter, passes filter test
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.1%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.05%
Ni ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%
应用
用于配制筛选或优化的结晶级 ADA 缓冲液
特征
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，< 5 ppb 总有机碳，无细菌（<1 细菌（CFU/ml）），无热原（<0.03 内毒素（EU/ml）） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)
描述
ADA（缓冲器）
同义词：N-(2-乙酰氨基)亚氨基二乙酸；
N-(氨基甲酰基甲基)亚氨基二乙酸
C6H10N2O5
H2NCOCH2N(CH2CO2H)2
190.16 先生
CAS号 [26239-55-4]
EC 编号 247-530-0
贝尔斯坦注册号 1787181
默克 14,147
纯度≥ 99.0%
MDL 编号 MFCD00008031
PubChem 物质 ID 24845005
pKa = 6.6 在 25°C
ADA 的有用 pH 范围为 5.6 – 7.5
使用 NaOH 将 HR2-507 滴定至所需的 pH
使用 NaOH 将 HR2-817 滴定至 pH 6.5
HR2-507 测得的电导率范围：23.6 – 28.6 mS/cm at 25°C
HR2-507 测得的折射率范围：1.35009 – 1.35044 at 20°C
HR2-817 测得的电导率范围：42.2 – 50.4 mS/cm at 25°C
HR2-817 测得的折射率范围：1.36787 – 1.36913 at 20°C
ADA 缓冲液用于 Hampton Research MembFac 结晶试剂盒。
总杂质 不溶物，通过过滤测试
铝≤0.0005%
≤0.00001%
钡≤0.0005%
铋≤0.0005%
钙≤0.001%
镉≤0.0005%
氯≤0.1%
钴≤0.0005%
铬≤0.0005%
铜≤0.0005%
铁≤0.0005%
钾≤0.005%
镁≤0.0005%
锰 ≤0.0005%
钼≤0.0005%
钠≤0.05%
镍≤0.0005%
铅≤0.0005%
SO4 ≤0.005%
锶≤0.0005%
锌≤0.0005%
ADA
CAT NO
HR2-507
NAME
DESCRIPTION
100 mL
PRICE
$112.00
cart quote
CAT NO
HR2-817
NAME
DESCRIPTION
100 mL
PRICE
$121.00
cart quote
Support Material(s)
HR2-507 ADA 0.5 M solution SDS
HR2-817 ADA 1.0 M pH 6.5 solution SDSCertificate Of Analysis
Related Item(S)
- Sodium hydroxide
- Individual StockOptions ADA Reagents
- StockOptions ADA Buffer Kit
References
1. Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin. Arni, RK; Padmanabhan, K; Padmanabhan, KP; Wu, TP; Tulinsky, A. Biochemistry 32, 4727- 4737, 1993.
2. Structures of Three Crystals Forms of the Sweet Protein Thaumatin. Ko, TP; Day, J; Greenwood, A; McPherson, A. Acta Crystallogr D 50, 813- 825, 1994.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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