Why Sandwich Boxes? They allow one to use very, very large drops. They are optically superior to plastic plates and glass slides, and offer different equilibration kinetics than other crystallization plates. Each of the Sandwich Box components can be washed and reused so there is little waste with the Sandwich Box Setup. These plates can be used during screening and optimization, but are best suited for final optimization and production of crystals. Since the glass plates can be removed from the boxes, crystal mounting is convenient. The siliconized 9 well depression plates are also useful for heavy atom soaks, cryo solution dilution and transfers, and seeding experiments where serial dilutions are involved. Sandwich Box Setups are available as a complete set or as individual components so one can customize the system to meet their needs. Square cover slides can be used to seal individual reservoirs but are not typically used in a crystallization setup. The Sandwich Box Setup consists of a square plastic box (4 5/16” x 4 5/16” x 1 1/8”), a polystyrene plate support, and a siliconized, glass plate (4” L x 3 3/8” W (100 mm x 85 mm)) with nine concave depressions (7/8” O.D. x 1/4” D (22 mm x 7 mm)).

Drop sizes up to 800 microliters are possible with the Sandwich Box Setup.

9 孔玻璃板和夹心盒设置
应用
坐滴结晶
播种
重原子浸泡
低温转移
大液滴产生用于中子衍射研究的大晶体
特征
硅化 9 孔玻璃板
凹井
聚苯乙烯盒和支架
描述
设置夹心盒时，将25毫升结晶试剂或脱水剂移入夹心盒底部，然后将支架放入盒中。 9 孔凹陷板放置在支持物上。将样品和试剂滴吸入凹陷处，夹心盒的盖子用真空油脂密封。

为什么是三明治盒？它们允许使用非常非常大的液滴。它们在光学上优于塑料板和载玻片，并提供与其他结晶板不同的平衡动力学。每个夹心盒组件都可以清洗和重复使用，因此夹心盒设置几乎不会造成浪费。这些板可用于筛选和优化，但最适合晶体的最终优化和生产。由于玻璃板可以从盒子中取出，水晶安装很方便。硅化 9 孔凹陷板也可用于重原子浸泡、低温溶液稀释和转移，以及涉及连续稀释的接种实验。三明治盒设置可作为一套完整的组件或作为单独的组件使用，因此您可以自定义系统以满足他们的需求。方形盖玻片可用于密封单个储液槽，但通常不用于结晶设置。夹心盒装置包括一个方形塑料盒（4 5/16” x 4 5/16” x 1 1/8”）、一个聚苯乙烯板支架和一块硅化玻璃板（4” L x 3 3/8 ” W (100 mm x 85 mm))，带有九个凹面（7/8” OD x 1/4” D (22 mm x 7 mm)）。

使用夹心盒设置可实现高达 800 微升的液滴尺寸。

CAT NO

Sandwich Box Setup

Siliconized 9 Well Glass Plate

Sandwich Box with cover

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3. Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments. Acta Crystallographica Section D Structural Biology. 75. 10.1107/S2059798319012695.   Andersson, Rebecka & Safari, Cecilia & Båth, Petra & Bosman, Robert & Shilova, Anastasya & Dahl, Peter & Ghosh, Swagatha & Dunge, Andreas & Kjeldsen-Jensen, Rasmus & Nan, Jie & Shoeman, Robert & Kloos, Marco & Doak, R. & Mueller, Uwe & Neutze, Richard & Brändén, Gisela. (2019).