PROTEOSTAT® PDI assay kit 蛋白二硫键异构酶检测试剂盒

产品中心 > 生命科学 > 药物筛选 > 蛋白分析

PROTEOSTAT® PDI assay kit
蛋白二硫键异构酶检测试剂盒

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

PROTEOSTAT® PDI assay kitPROTEOSTAT®  PDI assay kit                              蛋白二硫键异构酶检测试剂盒

蛋白二硫键异构酶检测试剂盒

PROTEOSTAT® PDI 检测试剂盒能够以稳定且高通量的方式定量检测PDI酶活性,适用于从化学库中筛选PDI抑制剂,为筛选PDI酶活性调节剂提供了一种简单、均相的检测方法。

本试剂盒在二硫苏糖醇(DTT)存在的条件下,PDI酶催化胰岛素聚集体的形成,随后与发射红色荧光的 PROTEOSTAT® PDI检测染料结合,通过监测胰岛素减少来实现对PDI酶活性的检测。

相对于使用比浊法检测PDI酶活性,基于荧光的检测法有着极大改进的信号检测窗口和检测下限,以及优越的 Z' 因子(>0.8)。检测中板内和板间CV值为3-5%。

本试剂盒中包含人重组PDI酶(EC 5.3.4.1)、杆菌肽(PDI抑制剂)以及监测PDI酶活性变化所需的全部试剂。

◆产品详情


基础信息


别称:蛋白二硫键异构酶(Protein disulfide isomerase)检测试剂盒

应用:荧光检测

储存条件:避光。避免反复冻融。

运输方式:干冰运输

长期储存温度:–80°C

特点:本试剂盒为筛选蛋白二硫键异构酶(PDI)酶活性的调节剂提供了一种简单、均相的检测方法。

UniProt ID:P07237(人)

监管状态:RUO – 仅供研究使用

技术信息/产品说明:Enzo和PROTEOSTAT®是Enzo Life Sciences, Inc. 的商标。

技术信息/产品说明:Enzo的部分产品和产品应用受美国、外国专利及正在申请的专利保护。



品质保证


(1)按照手册中的步骤, PROTEOSTAT® PDI检测试剂盒可用于检测以下样品:

(1)① 含PDI的胰岛素;② 不含PDI的胰岛素;③ 含有PDI和杆菌肽的胰岛素。

(2)Z' 因子高于0.8。

(3)PDI活性至少可被杆菌肽抑制50%。



使用/稳定性


在产品标签注明的日期前,试剂盒组分在适当的储存条件下可稳定保存。

可存放于-20°C的无霜冰箱中,或在–80°C下长期保存。



组分


● PROTEOSTAT® PDI 检测试剂

● PDI(人)(重组)

● 胰岛素(冻干)

● 杆菌肽(抑制剂对照,冻干)

● PBE 缓冲液

● 终止试剂

● DTT(10 mM)

● 去离子水


※ 本页面产品仅供研究用,研究以外不可使用。

参考文献


 1. 

Thioredoxin reductase is a major regulator of metabolism in leukemia cells: S. Karunanithi, et al.; Oncogene 41388 (2021), Abstract;


 2.

Molecular docking‐assisted screening reveals tannic acid as a natural protein disulphide isomerase inhibitor with antiplatelet and antithrombotic activities: L. Ren, et al.; J. Cell. Mol. Med. 24, 14257 (2020), AbstractFull Text

 3.

PDI knockdown inhibits seizure activity in acute seizure and chronic epilepsy rat models via S-nitrosylation-independent thiolation on NMDA receptor: A.R. Jeon, et al.; Front. Cell. Neurosci. 12, 438 (2018), Application(s): Hippocampal tissue lysates, AbstractFull Text

 4.

The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition: S. Hager, et al.; Cell Death Dis. 9, 1052 (2018), AbstractFull Text

 5.

Tissue Factor Coagulant Activity is Regulated by the Plasma Membrane Microenvironment: Y. Yu, et al.; Thromb. Haemost. 118, 990 (2018), Application(s): In-vitro activity assay, Abstract;


 6.

Curcumin and Curcuma longa L. extract ameliorate lipid accumulation through the regulation of the endoplasmic reticulum redox and ER stress: H.Y. Lee, et al.; Sci. Rep. 7, 6513 (2017), Application(s): PDI assay of tissue lysates, AbstractFull Text

 7.

Nox4 regulates the eNOS uncoupling process in aging endothelial cells: H.Y. Lee, et al.; Free Radic. Biol. Med. 113, 26 (2017), Application(s): PDI assay of HUVEC cell lysates, Abstract;


 8.

PDI regulates seizure activity via NMDA receptor redox in rats: J.Y. Kim, et al.; Sci. Rep. 7, 42491 (2017), Application(s): Hippocampal tissue lysates, Abstract; Full Text

 9.

Protein disulfide isomerase regulates renal AT1 receptor function and blood pressure in rats: X. Wang, et al.; Am. J. Physiol. Renal. Physiol. 313, F461 (2017), Application(s): Renal tissue lysates, AbstractFull Text

10.

Requirement of zinc transporter SLC39A7/ZIP7 for dermal development to fine-tune endoplasmic reticulum function by regulating protein disulfide isomerase: B.H. Bin, et al.; J. Invest. Dermatol. 137, 1682 (2017), Application(s): ER fractions from murine osteoblasts, Abstract;


11. 

Succination of protein disulfide isomerase links mitochondrial stress and endoplasmic reticulum stress in the adipocyte during diabetes: A.M. Manuel, et al.; Antioxid. Redox Signal. 27, 1281 (2017), Application(s): 3T3-L1 adipocytes and adipose tissue lysates, AbstractFull Text

12.

Adverse outcomes associated with cigarette smoke radicals related to damage to protein disulfide isomerase: H. Kenche, et al.; J. Biol. Chem. 291, 4763 (2016), Abstract;


13.

Adverse Outcomes Associated with Cigarette Smoke Radicals Related to Damage to Protein-disulfide Isomerase: H. Kenche, et al.; J. Biol. Chem. 291, 4763 (2016), Application(s): in-vitro assay with recombinant PDI, AbstractFull Text

14.

Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity: K. Mätlik, et al.; Cell Death Dis. 6, e2032 (2015), AbstractFull Text

15.

Methods of measuring protein disulfide isomerase activity: a critical overview: M.M. Watanabe, et al.; Front. Chem. 2, 73 (2014), Application(s): Review on available assays to measure PDI activity, AbstractFull Text

16.

N-octanoyl dopamine treatment of endothelial cells induces the unfolded protein response and results in hypometabolism and tolerance to hypothermia: E. Stamellou, et al.; PLoS One 9, e99298 (2014), AbstractFull Text

17.

The protein disulfide isomerases PDIA4 and PDIA6 mediate resistance to cisplatin-induced cell death in lung adenocarcinoma: G. Tufo, et al.; Cell Death Differ. 21, 685 (2014), Application(s): Evaluation of PDI activity in whole extracts and ER fractions, Abstract;


18.

Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase: F. Langer, et al.; Blood 121, 2324 (2013), Abstract;


19.

Oxidative processing of latent Fas in the endoplasmic reticulum controls the strength of apoptosis: V. Anathy, et al.; Mol. Cell. Biol. 32, 3464 (2012), Abstract;



◆一般参考文献


 1. 

Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase: S. Abromaitis & R.S. Stephens; PLoS Pathog. 5, e1000357 (2009), Abstract;


 2.

Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages: C. X. Santos, et al.; J. Leukoc. Biol. 86, 989 (2009), Abstract;


 3.

The proteostasis boundary in misfolding diseases of membrane traffic: D.M. Hutt, et al.; FEBS Lett. 583, 2639 (2009), Abstract;


 4.

Increasing melanoma cell death using inhibitors of protein disulfide isomerases to abrogate survival responses to endoplasmic reticulum stress: P.E. Lovat, et al.; Cancer Res. 68, 5363 (2008), Abstract;


 5.

Targeting homeostatic mechanisms of endoplasmic reticulum stress to increase susceptibility of cancer cells to fenretinide-induced apoptosis: the role of stress proteins ERdj5 and ERp57: M. Corazzari, et al.; Br. J. Cancer 96, 1062 (2007), Abstract;


 6.

Coupling endoplasmic reticulum stress to the cell death program: R.V. Rao, et al.; Cell Death Differ. 11, 372 (2004), Abstract;


 7.

Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion: R. Barbouche, et al.; J. Biol. Chem. 278, 3131 (2003), Abstract;


 8.

Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells: C.H. Davis, et al.; Infect. Immun. 70, 3413 (2002), Abstract;


 9.

Protein-disulfide isomerase-mediated reduction of the A subunit of cholera toxin in a human intestinal cell line: P.A. Orlandi; J. Biol. Chem. 272, 4591 (1997), Abstract;


10.

Acceleration of reactivation of reduced bovine pancreatic ribonuclease by a microsomal system from rat liver: R.F. Goldberger, et al.; J. Biol. Chem. 238, 628 (1963), Abstract;



产品列表

产品编号 产品名称 产品规格 产品等级 备注
ENZ-51024-KP002 PROTEOSTAT PDI assay kit 
PROTEOSTAT PDI检测试剂盒
2×96 tests
ENZ-51024-KP050 PROTEOSTAT PDI assay kit 
PROTEOSTAT PDI检测试剂盒
50 tests

免责声明

1. 本公司密切关注本网站发布的内容,但不保证发布内容的准确性、完整性、可靠性和最新性等。

2. 本公司不保证使用本网站期间不会出现故障或计算机病毒污染的风险。

3. 无论何种原因,使用本网站时给用户或第三方造成的任何不利或损害,本公司概不负责。此外,对于用户与其他用户或第三方之间因本网站发生的任何交易、通讯

3. 纠纷,本公司概不负责。

4. 本网站可提供的所有产品和服务均不得用于人体或动物的临床诊断或治疗,仅可用于科研等非医疗目的。如任何用户将本网站提供的产品和服务用临床诊断或治

4. 疗,以及他特定的用途或行为,本公司概不保证其安全性和有效性,并且不负任何相关的法律责任。