Megazyme内切木聚糖酶测定试剂盒(xylx6法)

Megazyme内切木聚糖酶测定试剂盒(xylx6法)
上海金畔生物科技有限公司
品牌:Megazyme

英文名:endo-Xylanase Assay Kit (XylX6 Method)

货号:K-XYLX6-2V

规格:200 assays (manual) / 400 assays (auto-analyser)

 The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Colourimetric method for the determination of endo-1,4-β-xylanase in any 
sample type including crude enzyme extracts, fermentation broths or 
commercial enzyme preparations

Principle:
                                               (endo-1,4-β-xylanase)
(1) 3-Ketobutylidene-GX5-β-PNP + H2O → Blocked-GXY + X(5-Y)-β-PNP

                            (β-glucosidase)
(2) X(5-Y)-β-PNP + H2O → D-xylose + PNP

       (alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

 

Kit size:
K-XylX6-1V 100 assays (manual) / 200 (auto-analyser)
                                        or
K-XylX6-2V 200 assays (manual) / 400 (auto-analyser)

Method:                         Spectrophotometric at 400/405 nm
Total assay time:           10 min
Detection limit:                5.3 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley
malt analysis
Method recognition:     Novel method

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Megazyme产品所有分类:
生物及食品酶法试剂盒(Assay Kits)
膳食纤维/淀粉(Dietary Fiber/Starch) 单/双糖(Mono/Disaccharides) 多糖(Polysaccharides) 醇类(Alcohols) 有机酸(Organic Acids) 亚硫酸盐/氮(Sulfite/Nitrogen) 活性酶(Enzyme Activity) 试剂混合物(Reagent Mixtures) 其它(Other)
酶(Enzymes)
活性酶(Carbohydrate Active enZYmes )
淀粉酶(Amylases )
阿拉伯树胶酸(Arabinanases)
阿拉伯呋喃(Arabinofuranosidases)
纤维二糖水解(Cellobiohydrolases)
纤维素酶(Cellulases)
酯酶(Esterases)
果糖酶&果糖苷酶(Fructanases & Fructosidases)
岩藻糖苷酶(Fucosidases)
半乳聚糖酶(Galactanases)
半乳糖苷酶(Galactosidases)
葡聚糖酶(Glucanases)
葡萄糖苷酶(Glucosidases)
葡糖醛酸糖苷(Glucuronidases)
已糖胺酶类(Hexosaminidiases)
裂解酶(Lyases)
甘露聚糖酶(Mannanases)
甘露糖苷酶(Mannosidases)
支链淀粉酶(Pullulanases)
唾液酸酶(Sialidases)
木聚糖酶(Xylanases)
糖苷酶(Xylosidases)
木葡聚糖酶(Xyloglucanases)
多聚半乳糖醛酸(内切)酶(Polygalacturonases)
其它酶(Other Activities)
分析酶(Analytical Enzymes)
脱氢酶(Dehydrogenases)
异构酶(Isomerases)
激酶(Kinases)
磷酸酶(Phosphatases)
蛋白酶(Proteases)
其他活性酶(Other Activities)
糖类活性酶(Glycobiology Enzymes)
酶底物( Chromogenic Substra