NISSUI日水RPMI 1640 Medium培养基

NISSUI日水RPMI 1640 Medium

中文名称:RPM1640培养基2
英文品名:RPM1640 Medium ②
包装规格:100g
用途:
1、粉末组织培养基,可以进行高压蒸气消毒,不含L-谷酰胺和碳酸氢钠。
2、本培养基可用作老鼠和人的白血病细胞的初级细胞培养和菌株细胞的后续培养,此外还可用于包括KATO-Ⅲ细胞在内的各种人体癌细胞的培养。
使用方法:
把10.2g本产品溶解于蒸馏水中,使液体总量达到1000mL。等培养基完全溶解后在121℃条件下高压蒸气消毒15分钟。经过高压蒸气消毒后冷却至室温状态。然后在密封状态下向培养基中加入适量的经过高压蒸气消毒的10%碳酸氢钠,(当加入18~30mL时,CO2气体含量下降至5%以下、在温度为37℃时pH值为7.1~7.4)。如果不是马上使用,请使用密封垫圈将其密封并保存在低温(2~10℃)之处。在使用前应另外加入经过过滤消毒的L-谷酰胺0.292g。使用前请根据不同目的添加适量血清。
贮藏方法、使用期限:
请把本培养基粉末用密封垫圈密封保存在干燥低温(2~10℃)之处。使用期限为一年。

Directions
Dissolve 10.2 g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121°C for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate. (pH will be 7.1-7.4 at 37°C, in an atmosphere of 5% CO2 in air by adding 18-30 mL of sodium bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.

Summary
RPMI 1640 is a medium for suspension culture investigated by George E. Moore, former director of Roswell Park Memorial Institute. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to overcome the difficulties associated with filtersterilized media, such as contamination by Mycoplasma. This medium has growth promoting effect to primary cultures of mouse and human leukemia cells, various kinds of cell lines and KATO-III cells.
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician. Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.

Formula

Components
In 10.2 g/L
L-Arginine Hydrochloride
240.0 mg
L-Asparagine, H2O
56.8 mg
L-Asparatic Acid
20.0 mg
L-Cysteine Hydrochloride, H2O
72.9 mg
L-Glutamic Acid
20.0 mg
Succinic Acid
46.0 mg
Sodium Succinate,6 H2O
164.0 mg
Glutathione
1.0 mg
Glycine
10.0 mg
L-Histidine Hydrochloride, H2O
20.3 mg
L-Hydroxyproline
20.0 mg
L-Isoleucine
50.0 mg
L-Leucine
50.0 mg
L-Lysine Hydrochloride
40.0 mg
L-Methionine
15.0 mg
L-Phenylalanine
15.0 mg
L-Proline
20.0 mg
L-Serine
30.0 mg
L-Threonine
20.0 mg
L-Tryptophan
5.0 mg
L-Tyrosine
20.0 mg
L-Valine
20.0 mg
Biotin
0.2 mg
Calcium Pantothenate
0.25 mg
Choline Chloride
3.0 mg
Folic Acid
1.0 mg
Inositol
35.0 mg
Nicotinamide
1.0 mg
p-Aminobenzoic Acid
1.0 mg
Pyridoxine Hydrochloride
1.0 mg
Riboflavin
0.2 mg
Thiamin Hydrochloride
1.0 mg
Cyanocobalamin
0.005 mg
Sodium Chloride
6,000.0 mg
Potassium Chloride
400.0 mg
Calcium Nitrate
69.5 mg
Monosodium Phosphate
677.0 mg
Magnesium Sulfate
48.8 mg
Glucose
2,000.0 mg
Phenol Red
5.0 mg

00883 NISSUI 日本日水 RPMI 1640液体培养基RPMI 1640 Liquid

产品编号:00883
中文名称:RPMI 1640液体培养基
英文品名:RPMI 1640 Liquid
包装规格:500ml
用途:液态组织培养基

使用方法:
RPMI 1640 Liquid“日水”是根据由RPMI(Roswell Park Memorial Institute)的Director即Dr.George E.Moore开发出来的浮游细菌培养基制造而成的液态培养基。本培养基被用作老鼠和人的白血病细胞的第一代培养基以及株的第二代培养基。
在使用前,请根据不同目的添加适量血清。

组成(mg/L):
RPMI 1640液体培养基RPMI 1640 Liquid编号:00883 

硝酸钙(四水盐)……………100
氯化钾…………………………400
硫酸镁(无水)………………48.84
氯化钠…………………………6000
磷酸一氢二钠(无水)………800
L-精氨酸盐酸盐………………200
L-天门冬酰胺…………………50
L-天冬氨酸……………………20
RPMI 1640液体培养基RPMI 1640 Liquid编号:00883 

L-胱氨酸二盐酸盐……………65.15
L-谷氨酸………………………20
L-谷氨酰胺……………………300
甘氨酸…………………………10
L-组氨酸………………………15
L-羟脯氨酸……………………20
L-异亮氨酸……………………50
L-赖氨酸盐酸盐………………40
L-甲硫氨酸……………………15
L-苯基丙氨酸…………………15
L-脯氨酸………………………20
L-丝氨酸………………………30
L-苏氨酸………………………20
L-色氨酸………………………5
L-酷氨酸二钠(二水盐)……28.83
L-缬氨酸………………………20
生物素…………………………0.2
泛酸钙…………………………0.25
氯化胆碱………………………3

RPMI 1640液体培养基RPMI 1640 Liquid编号:00883 

叶酸……………………………1
肌醇……………………………35
烟酰胺…………………………1
盐酸吡哆醛……………………1
核黄酸………………………0.2
盐酸硫胺………………………1
维他命B12……………………0.005
D.葡萄糖……………………2000
对氨基苯甲酸…………………1
谷胱甘肽(还原型)…………1
酚红(钠盐)………………5.3
碳酸氢钠……………………2000

贮藏方法、使用期限:
保存在低温(2~8℃)之处。使用期限为一年。

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