Oxoid代理 CM0325B Oxoid 平板计数琼脂培养基 PLATE COUNT AGAR
货号:CM0325B
中文名称:平板计数琼脂培养基(PCA)
规格:500G
Thermo Scientific™平板计数琼脂(干粉),型号:CM0325
CM0325B平板计数琼脂(干粉)培养基采用 Thermo Scientific™ Oxoid™ 平板计数琼脂(干粉)确定食品、乳制品、废水和具有卫生重要性的其他材料中微生物的平板计数。平板计数琼脂由Buchbinder 等人1 研制,经以下机构推荐:APHA2、AOAC3 和 PHLS4。平板计数琼脂的质量控制 (APHA) 包括根据 ISO 11133:2014 进行测试。5
认证/合规:Industrial Reference: NMKL.
描述:Plate Count Agar
形式形式:Powder
类型:Media
产量:For 28.6L medium
Dehydrated Culture Media
PLATE COUNT AGAR
TRYPTONE GLUCOSE YEAST AGAR
Code: CM0325
A medium for the enumeration of viable organisms in food, animal feed and water samples
用于计数食品、动物饲料和水样中活生物体的介质
Typical Formula* |
gm/litre |
Tryptone |
5.0 |
Yeast extract |
2.5 |
Glucose |
1.0 |
Agar |
9.0 |
pH 7.0 ± 0.2 @ 25°C |
* Adjusted as required to meet performance standards
Directions
Add 17.5g to 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Description
Plate Count Agar is equivalent to the medium recommended by the APHA1, PHLS 2 and ISO3,4 for the plate count of micro-organisms in food.
The APHA method consists of the preparation of pour plates using diluted samples, and counting colonies after incubation. Incubation is for 48 hours at 32°C or at 35°C for the Standard Plate Count. For the enumeration of micro-organisms with other temperature requirements, plates may also be incubated for 7-10 days at 5-7°C: for 3-5 days at 20°C; for 2-3 days at 45°C; or for 48 hours at 55°C. See the APHA1 publication for details.
Samples should be tested following the method described in the appropriate standard.
The quality control of Plate Count Agar includes testing in accordance with ISO 11133:20145.
描述
平板计数琼脂相当于 APHA1、PHLS 2 和 ISO3,4 推荐的用于食品中微生物平板计数的培养基。
APHA 方法包括使用稀释样品制备倾倒板,并在孵育后计数菌落。 标准板计数在 32°C 或 35°C 下孵育 48 小时。 对于有其他温度要求的微生物计数,板也可以在5-7°C下孵育7-10天:在20°C下孵育3-5天; 在 45°C 下 2-3 天; 或在 55°C 下放置 48 小时。 有关详细信息,请参阅 APHA1 出版物。
样品应按照相应标准中描述的方法进行测试。
平板计数琼脂的质量控制包括根据 ISO 11133:20145 进行的测试。
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的板储存在 2-8°C。
外貌
脱水介质:稻草色、自由流动的粉末
制备培养基:稻草色凝胶
Quality control
Positive controls: | Expected results |
Escherichia coli ATCC® 25922 * WDCM00013 |
Good growth; straw coloured colonies |
Bacillus subtilis ATCC® 6633* WDCM00003 |
Good growth; straw coloured colonies |
Staphylococcus aureus ATCC® 25923* WDCM00034 |
Good growth; straw coloured colonies |
Negative control: | |
Uninoculated medium | No change |
* This organism is available as a Culti-Loop®
Quality Control
Compare with previous lot/batch using pasteurised and raw milk samples, incubated at 32-35°C for 48 hours.
Precautions
Ensure that the procedures and media used in milk product testing comply with the National Regulations required for each country.
质量控制
与之前使用巴氏杀菌和原料奶样品的批次/批次相比,在 32-35°C 下孵育 48 小时。
防范措施
确保乳制品测试中使用的程序和介质符合每个国家/地区要求的国家法规。
Reference 参考文献
1. American Public Health Association (1978) Standard Methods for the Examination of Dairy Products. 14th Edn. APHA Inc. Washington DC.
2. PHLS Standard methods for the Microbial Examination of Food and Dairy Samples (1999) Aerobic plate count at 30°C – F10 & F11
3. International Organization for Standardization. ISO 4833 Parts 1 and 2, Microbiology of the food chain – Horizontal method for the enumeration of microorganisms
4. International Organization for Standardization. Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of psychrotrophic microorganisms.
5. International Organization for Standardization. ISO 11133:2014 Microbiology of food, animal feed and water – Preparation, production, storage and performance testing of culture media
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