Oxoid CM1032B 心浸出液肉汤 HEART INFUSION BROTH成分表

Oxoid CM1032B 心浸出液肉汤 HEART INFUSION BROTH成分表

HEART INFUSION BROTH

Code: CM1032

a highly nutritious liquid medium recommended for the cultivation of fastidious organisms

Typical Formula* gm/litre
Beef heart infusion solids 17.5
Proteose peptone 10.0
Glucose 2.0
Sodium chloride 5.0
Di-sodium phosphate 2.5
pH 7.4 ± 0.2 @ 25°C
* Adjusted as required to meet performance standards

Directions
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121ºC for 15 minutes.

Description
Heart Infusion Broth has been developed to give the same performance characteristics as Brain Heart Infusion (BHI) Broth. However, as bovine brain is a specified risk material, the exclusion of it from the Heart Infusion Broth means that the regulatory requirements when using it are lower.
The medium has been developed to give equivalent performance to Brain Heart Infusion but the exclusion of calf brain infusion means that Heart Infusion Broth carries a lower regulatory burden.

Simple additions may be made to the medium to make it suitable for the cultivation of yeasts and moulds and for use in blood culture.
A highly nutritious infusion medium recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms.

描述
Heart Infusion Broth 的开发提供了与 Brain Heart Infusion (BHI) Broth 相同的性能特征。 然而,由于牛脑是一种特定的风险材料,将其排除在 Heart Infusion Broth 之外意味着使用它时的监管要求较低。
该培养基的开发可提供与 Brain Heart Infusion 相同的性能,但排除小牛脑灌注意味着 Heart Infusion Broth 的监管负担较低。

可以对培养基进行简单的添加,使其适合酵母和霉菌的培养以及用于血培养。
一种高营养的输液培养基,推荐用于培养链球菌、肺炎球菌、脑膜炎球菌和其他挑剔的生物。

Technique
Heart Infusion Broth can be supplemented with yeast extract, haemin and menadione to improve the growth of Bacteroides spp.A medium for blood culture can be prepared by adding 1g of agar per litre of Heart Infusion. Ensure that the agar is uniformly distributed in the sterile broth before dispensing into bottles. Supplements to improve the recovery of organisms from blood can be added before sterilisation or aseptically post-sterilisation. Co-enzyme 1 (NAD), penicillinase and p-amino benzoic acid are examples1. The addition of anti-microbial agents such as cycloheximide and chloramphenicol also make this medium suitable for the cultivation of yeasts and moulds.

技术
Heart Infusion Broth 可添加酵母提取物、血红素和甲萘醌,以促进拟杆菌属的生长。1 每升 Heart Infusion 添加 1g 琼脂可制备血培养培养基。 确保琼脂在分配到瓶子之前均匀分布在无菌肉汤中。 可以在灭菌前或灭菌后添加改善血液中微生物回收率的补充剂。 辅酶 1 (NAD)、青霉素酶和对氨基苯甲酸是示例 1。 添加抗微生物剂如放线菌酮和氯霉素也使该培养基适用于酵母和霉菌的培养。

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium in the dark below 20°C.

储存条件和保质期
脱水培养基应在 10-30°C 下储存,并在标签上的有效期前使用。
将制备好的培养基储存在 20°C 以下的黑暗中。

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured liquid

外貌
脱水介质:稻草色、自由流动的粉末
制备培养基:稻草色液体

Quality control

Positive controls: Expected results
Streptococcus pneumoniae ATCC® 6303 * Turbid growth
Candida albicans ATCC 10231 Turbid growth
Negative control:
Uninoculated medium No change
* This organism is available as a Culti-Loop®

Precautions
Heart Infusion Broth should only be used for in vitro diagnostic purposes.

References 参考文献
1. Bridson, E.Y. (Ed) (1998), The Oxoid Manual, 8th Edition, Oxoid Ltd, UK, pp. 2-51.2-52.

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