Oxoid现货供应 Oxoid CM0479B 远藤氏琼脂培养基
OXOID CM0479B远藤氏琼脂基础
英文名称：ENDO AGAR BASE
产地/品牌：英国OXOID
规 格：500g
货 号：CM0479B
ENDO AGAR BASE
Code: CM0479
A modified medium requiring the addition of basic fuchsin to form Endo Agar.
一种改良培养基，需要添加碱性品红以形成远藤琼脂。
| Typical Formula* |
gm/litre |
| Peptone |
10.0 |
| Lactose |
10.0 |
| Di-potassium phosphate |
3.5 |
| Sodium sulphite |
2.5 |
| Agar |
10.0 |
| pH 7.5 ± 0.2 @ 25°C |
* Adjusted as required to meet performance standards
Directions
Suspend 36g in 1 litre of distilled water. Add 6ml (or as directed by the supplier) of a 10% w/v solution of basic fuchsin BR0050 (50:50 ethanol/distilled water). Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. Plates should be stored in the dark to preserve their pale pink colour.
碱性品红是一种潜在的致癌物质，应注意避免吸入粉末染料和污染皮肤。
描述
Endo Agar 是一种历史悠久的培养基，最初设计用于分离伤寒杆菌。 用于此目的的更可靠的培养基已经发展出来，现在该培养基用于区分发酵乳糖和非发酵乳糖的肠道生物，特别是在确认大肠菌的推定试验期间。 乳糖发酵生物（如大肠杆菌）产生酸和醛，导致菌落和周围培养基呈特征性的红色。
BASIC FUCHSIN IS A POTENTIAL CARCINOGEN AND CARE SHOULD BE TAKEN TO AVOID INHALATION OF THE POWDERED DYE AND CONTAMINATION OF THE SKIN.
Description
Endo Agar is a long established medium which was originally devised for the isolation of the typhoid bacillus. More reliable media for this purpose have since been evolved, and the medium is now used for the differentiation of lactose fermenting and non-lactose fermenting intestinal organisms, particularly during confirmation of the presumptive test for coliforms. Production of both acid and aldehyde by lactose fermenting organisms, such as Escherichia coli, gives rise to the characteristic red coloration of the colony and the surrounding medium.
碱性品红是一种潜在的致癌物质，应注意避免吸入粉末染料和污染皮肤。
描述
Endo Agar 是一种历史悠久的培养基，最初设计用于分离伤寒杆菌。 用于此目的的更可靠的培养基已经发展出来，现在该培养基用于区分发酵乳糖和非发酵乳糖的肠道生物，特别是在确认大肠菌的推定试验期间。 乳糖发酵生物（如大肠杆菌）产生酸和醛，导致菌落和周围培养基呈特征性的红色。
Technique
For the confirmation of presumptive tests with liquid media, subculture tubes showing gas, or acid and gas formation, onto an Endo Agar plate. Incubate for 24 hours at 35°C.
Lactose fermenting coliforms (e.g. Escherichia coli) give rise to deep red colonies which colour the surrounding medium and possess a golden metallic sheen.
Non-lactose fermenters form colourless translucent colonies, against the pink to colourless medium.
技术
为了确认使用液体培养基进行的假定测试，将显示气体或酸和气体形成的继代培养管置于 Endo 琼脂板上。 在 35°C 下孵育 24 小时。
乳糖发酵大肠菌群（例如大肠杆菌）会产生深红色菌落，使周围培养基着色并具有金色金属光泽。
非乳糖发酵罐形成无色半透明菌落，对粉红色至无色培养基。
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的板存放在 2-8°C 避光处。
外貌
脱水介质：稻草色、自由流动的粉末
制备培养基：稻草色凝胶
Quality control
| Positive controls: | Expected results |
| Escherichia coli ATCC® 25922 * | Good growth; red colonies, red medium, golden sheen |
| Enterobacter aerogenes ATCC® 13048 * | Good growth; pink / red coloured colonies no sheen |
| Negative control: | |
| Staphylococcus aureus ATCC® 25923 * | Inhibited |
* This organism is available as a Culti-Loop®
Precautions
Weigh out the basic fuchsin (BR0050) in a fume cupboard and avoid inhalation of the powder or contamination of the skin.
Keep the prepared medium away from light to avoid photo-oxidation.
Endo Agar is quoted by the American Public Health Association as a `Standard Methods’ medium for use in water1 and dairy products2. Windle Taylor3 recommended the medium for the isolation and differentiation of coli-aerogenes bacteria from water.
防范措施
在通风柜中称量碱性品红 (BR0050)，避免吸入粉末或污染皮肤。
使准备好的培养基远离光线，以避免光氧化。
Endo Agar 被美国公共卫生协会引用为用于水 1 和乳制品 2 的“标准方法”培养基。 Windle Taylor3 推荐用于从水中分离和分化产气大肠杆菌的培养基。
References 参考文献
1. American Public Health Association (1998) Standard Methods for the Examination of Water and Wastewater. 20th Edn. APHA Inc. Washington DC.
2. American Public Health Association (1978) Standard Methods for the Examination of Dairy Products. 14th Edn. APHA Inc. Washington DC.
3. Windle Taylor E. (1958) `The Examination of Waters and Water Supplies’ 7th ed., Churchill Ltd., London, pp. 417. 440-441, 780-781.
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