Wako 019-19741小胶质细胞/巨噬细胞特异性抗体Anti Iba1, Rabbit

Wako 019-19741小胶质细胞/巨噬细胞特异性抗体Anti Iba1, Rabbit

离子钙接头蛋白分子1抗体(Anti Iba1, Rabbit)以合成的的Iba1羧基末端序列为抗原,该序列是人,大鼠和小鼠Iba1蛋白质保守序列。本抗体(免疫组化用)特异性与小胶质细胞/巨噬细胞反应,免疫双重染色与单克隆抗体结合,可与GFAP单克隆抗体((胶质纤维酸性蛋白,Glial Fibrillary Acidic Protein)联合使用对脑组织和细胞培养物进行双重染色。
名词解释:
Iba1: ionized calcium binding adapter molecule 1 小胶质细胞/巨噬细胞特异性蛋白抗体
钙离子是已知的所有细胞,包括中枢神经系统(CNS)细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动,其中许多钙结合蛋白被划分成一个大的蛋白家族,EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白,在巨噬细胞和小胶质细胞中表达,并在这些细胞的活化过程中表达升高。
产品特点
S特异性检测小胶质细胞与/巨噬细胞,不与神经细胞与星形胶质细胞发生交叉反应。
可与人,大鼠,小鼠的Iba1反应
货号
品名
包装
用途
工作浓度
019-19741
Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry
50ug(100ul)
IH
1-2ug/ml
016-20001
Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting
50ug(100ul)
WB
0.5-1ug/ml
应用
石蜡切片应用方法:离子钙接头蛋白分子1抗体(Anti Iba1, Rabbit)
1. 固定
2. 用0.01M PBS缓冲液清洗二次
3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
4. 用0.01M PBS缓冲液清洗三次
5. 使用封闭液(含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液)室温孵育2小时
6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
7. 用0.01M PBS缓冲液清洗三次
8. 使用含有生物素标记的抗兔IgG(1:200)的封闭液室温孵育1小时
9. 用0.01M PBS缓冲液清洗三次
10. 使用Elite ABC试剂(含有试剂A1:50,试剂B1:50的0.01M PBS溶液)
11. 用0.01M PBS缓冲液清洗三次
12. 过氧化物酶溶液(含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液)

◆相关产品

016-20001  小胶质细胞Iba1标签抗体

 

产品编号No. 产品名称 等级 规格
019-19741 Iba1抗体、兔子(用于免疫细胞化学) 免疫化学用 50μg
016-20001 Iba1抗体、兔子(用于免疫印迹) 免疫化学用 50μg
015-25191 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64) 免疫化学用 50μL
010-26481 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64)、生物素相结合 免疫化学用 100μl
017-26491 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64)、FITC相结合 免疫化学用 100μl

 

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Wako 016-20001  Anti Iba1, Rabbit (for Western Blotting)  和光纯药  现货

产品编号 产品名称 产品规格 产品等级
013-26471 Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated小胶质细胞/巨噬细胞特异性蛋白抗体(结合红色荧光素635) 100μl for Immunochemistry
016-26461 Anti Iba1, Rabbit, Biotin-conjugated小胶质细胞/巨噬细胞特异性蛋白抗体(结合生物素) 100μl for Immunochemistry
017-26491 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) FITC-conjugated 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64)、FITC相结合 100μ for Immunochemistry
010-26481 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)Biotin-conjugated 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64)、生物素相结合 100μ for Immunochemistry
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016-20001 ANTI IBA1,RABBIT (FOR WESTERN BLOTTING) 小胶质细胞/巨噬细胞特异性蛋白抗体(免疫印迹) 50μg for Immunochemistry
019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 小胶质细胞/巨噬细胞特异性蛋白抗体(免疫组化) 50μg for Immunochemistry

 

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