Parabar 10312 (previously known as Paratone)-Hampton蛋白结晶

Parabar 10312 (previously known as Paratone)-Hampton蛋白结晶

Parabar 10312 (previously known as Paratone)-Hampton蛋白结晶

Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)

Parabar 10312 (previously known as Paratone)

Applications

  • Oil based cryoprotectant

Features

  • Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
  • Oil based cryoprotectant

Description

Sorry, Parabar 10312 is no longer available.  We’ve left this page up for reference only.  See HR2-861 Santovac Cryo Oil as a possible substitute.

Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1

Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.

HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.

The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.

Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.

Refractive index 1.48487 at 20°C.

Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.

Clear to slightly hazy is appearance.

Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512.

Paratone® is a registered trademark of Chevron Corporation.

Parabar 10312(以前称为 Paratone)
应用
油基冷冻保护剂
特征
晶体学文献中使用的同义词:Paratone® N、Paratone® 8277、润英联 V8512
油基冷冻保护剂
描述
抱歉,Parabar 10312 不再可用。我们保留此页面仅供参考。请参阅 HR2-861 Santovac Cryo Oil 作为可能的替代品。

Parabar 10312 是一种适用于小分子和大分子晶体学的粘性冷冻保护剂。 1

轻度空气不稳定的小分子化合物可以在惰性气氛下用 Parabar 涂层。 Parabar 保护的晶体样品可以在冷冻氮气流中低温冷却。 Parabar 也已成功用作生物大分子晶体的冷冻保护剂。在冷冻晶体学期间用于在晶体安装在冷冻环中后置换和减少晶体上的水(母液、试剂)的量。用 Parabar 涂层晶体可以最大限度地减少晶体的蒸发,并减少空气(氧气)对晶体的暴露和缓慢扩散。

HR2-643 Parabar 10312 不再可用,并已于 2021 年 5 月 11 日停产。

HR2-862 Parabar 10312 以 5 ml 装在琥珀色玻璃小瓶中,螺旋盖中带有玻璃涂抹棒。

通过以 50:50 的比例与石蜡油混合,可以降低 Parabar 的粘度,以改善对易碎针和刀片晶体的处理。

Parabar 是一种 Staudinger 分子量约为 20,000 的聚异丁烯混合物,含有约 20% 的聚合物。

20°C 时的折射率为 1.48487。

100°C 时的运动粘度为 641 cSt。 100°C 时的运动粘度比为 2.05。

外观清澈到略微朦胧。

晶体学文献中使用的同义词:Paratone® N、Paratone® 8277、润英联 V8512。

Paratone® 是雪佛龙公司的注册商标。

Parabar 10312

Parabar 10312 (previously known as Paratone)-Hampton蛋白结晶
Parabar 10312 (previously known as Paratone)-Hampton蛋白结晶

Support Material(s)

Parabar 10312 (previously known as Paratone)-Hampton蛋白结晶 HR2-862 Parabar 10312 User GuideParabar 10312 (previously known as Paratone)-Hampton蛋白结晶 HR2-643 HR2-862 Parabar 10312 SDS

Certificate Of Analysis

Related Item(S)

  • Perfluoropolyether Cryo Oil
  • Santovac Cryo Oil

References

1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.

2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.

3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.

4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.