Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-211
NDSB-211
Applications
- Crystallization grade NDSB-211 for formulating screens or for optimization
Features
- Efficiently solubilizes proteins at non-denaturing concentrations
- Useful in preventing protein aggregation
- Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
- Protein specific additive for solubility and stability
Description
The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.
Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.
Typical useful NDSB concentration in protein sample is 0.5-1.0 M.
NDSB-211
Synonyms: Dimethyl(2-hydroxyethyl)ammonium propane sulfonate or Non detergent sulfobetaine 211
C7H17NO4S
Mr 211.30
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)
应用
结晶级 NDSB-211 用于配制筛网或优化
特征
在非变性浓度下有效溶解蛋白质
有助于防止蛋白质聚集
非去污剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂
描述
NDSB 是一组两性离子化合物,可以减少聚集并有助于重新折叠包涵体和细菌表达系统中发现的蛋白质。
非去污剂磺基甜菜碱具有磺基甜菜碱亲水基团和短疏水基团,不能聚集形成胶束,因此 NDSB 不被视为去污剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率(高达 30%)。短疏水基团与磺基甜菜碱基团的电荷中和相结合,可提高膜蛋白的产量。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。
蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。
NDSB-211
同义词:二甲基(2-羟乙基)丙烷磺酸铵或非洗涤剂磺基甜菜碱211
C7H17NO4S
211.30 先生
纯度:≥99%
吸湿性
室温保存(+20 摄氏度)
HR2-793
CAT NO
HR2-793
NAME
DESCRIPTION
5 grams
PRICE
$116.00
cart quote
Support Material(s)
Certificate Of Analysis
References
1. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
2. Vuillard, L., et al. 1995. Biochem. J. 305-, 337.
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