By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.

Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.

Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.

Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.

Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

应用
核酸和蛋白质/核酸复合物的初级偏向稀疏矩阵结晶筛选
特征
核酸稀疏矩阵筛选
稀疏基质配方有效地对盐类、多元醇、聚合物、有机物、添加剂和 pH 值进行采样
pH 值范围 5.6 – 8.5
管或深井块格式
描述
Natrix、Natrix 2 和 Natrix HT 基于已公布的核酸和蛋白质-核酸复合物结晶试剂配方。各种锤头状核酶和其他核酶、RNA、DNA、RNA-药物复合物和 RNA-蛋白质复合物已使用 Natrix 方案结晶。

通过使用稀疏矩阵采样技术，Natrix 试剂盒允许使用非常小的核酸样本快速测试各种 pH 值、盐和沉淀剂。

Natrix 筛选的独特之处在于，Natrix 筛选不仅使用传统的核酸沉淀剂 MPD，还利用各种分子量（200、400、4,000、8,000）的聚乙二醇 (PEG) 以及 2-丙醇、聚乙烯乙二醇单甲醚 (PEG MME) 和 1,6-己二醇。许多聚合物和低分子量有机沉淀剂与各种单价盐结合作为沉淀剂。这种盐与低分子量有机物和多元醇的组合，以及不同链长 PEG 的使用，已被证明是生产核酸和蛋白质-核酸复合物晶体的成功组合。

Natrix 包含 48 种独特的试剂，每种试剂 10 毫升，基于 William Scott 于 1995 年首次描述的稀疏矩阵公式。

Natrix 2 是 Natrix 的扩展，包含 48 种独特的试剂，每种试剂 10 ml。 Natrix 2 是一种基于从结晶数据中提取模式以及 Berger 等人于 1996 年首次描述的试剂配方的有偏稀疏矩阵筛选。

Natrix HT 在单个 Deep Well 模块中包含来自 Natrix 和 Natrix 2 的每种试剂 1 ml。

即用型试剂经过无菌过滤和超纯 1 类水配制，使用最高纯度的盐、聚合物、有机物和缓冲液。个别试剂可通过 Hampton Research Custom Shop 购买。

Natrix

Natrix 2

Natrix HT

 HR2-116 Natrix Documents HR2-116 Natrix SDS HR2-117 Natrix 2 Documents HR2-117 Natrix 2 SDS HR2-131 Natrix HT Documents HR2-131 Natrix HT SDS Changes in Formulation Natrix Natrix 2 Natrix HT Natrix Formulation & Scoring Data

1. Scott, W. G., Finch, J.T., Grenfell, R., Fogg, J., Smith, T., Gait, M.J., Klug, A., Journal of Molecular Biology (1995) 250: 327-332.

2. Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with its cognate DNA. E. G. Kapetaniou, D. Kotsifaki, M. Providaki, M. Rina, V. Bouriotis and M. Kokkinidis. Acta Cryst. (2007). F63, 12-14.

3. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Forster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Furste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.

4. Combinatorial crystallization of an RNA-protein complex. Danielle Bodrero Hoggan, Jeffrey A. Chao, G. S. Prasad, C. David Stouta and James R. Williamson. Acta Cryst (2003). D59, 466-473.

5. Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X-ray diffraction analysis of a 5S rRNA A-helix racemate. Volker A. Erdmanna et al. Acta Cryst. (2007). F63, 839-843.

6. Berger, et al., A Highly Effective 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments. Acta Cryst. Section D. (1996) Vol. D52 Part 3, 465-468.

7. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Förster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Fürste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.

8. Adams, A., Nucleic Acids Research (2002) Vol. 30, 719-725.

9. Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-b receptor promoter DNA. Agarkar et al. Acta Cryst. (2009). F65, 1261-1263.

10. Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+. T.-H. Jang and H. H. Park. Acta Cryst. (2014). F70, 513-516 [ doi:10.1107/S2053230X1400510X ]