Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate
C₆H₁₃NO₄S • H₂O
M_r 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 329824236
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity ≥ 99.0%
pKa 6.1 at 25°C

Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.

Useful pH range of MES 5.2 – 7.1

Titrate HR2-587 to the desired pH using NaOH
HR2-587 Measured pH Range: 3.2 – 3.4 at 25°C
HR2-587 Measured Conductivity Range: 166.4 – 2440 µS/cm at 25°C
HR2-587 Measured Refractive Index Range: 1.34655 – 1.34731 at 20°C

HR2-787 has been titrated to pH 6.5 using NaOH
HR2-787 Measured Conductivity Range: 19.2 – 25.6 mS/cm at 25°C
HR2-787 Measured Refractive Index Range: 1.36113 – 1.36251 at 20°C

HR2-943-07 – MES monohydrate pH 5.8 – 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

Impurities:
Endotoxin ≤ 50 EU/g
Yeast and Mold ≤ 100 CFU/g
Bioburden ≤ 100 CFU/g
Al ≤ 5.0 ppm
As ≤ 3.0 ppm
Ba ≤ 5.0 ppm
Bi ≤ 5.0 ppm
Ca ≤ 20.0 ppm
Cd ≤ 5.0 ppm
Co ≤ 5.0 ppm
Cr ≤ 5.0 ppm
Cu ≤ 5.0 ppm
Fe ≤ 5.0 ppm
Li ≤ 5.0 ppm
Mg ≤ 5.0 ppm
Mn ≤ 5.0 ppm
Mo ≤ 5.0 ppm
Na ≤ 20.0 ppm
Ni ≤ 5.0 ppm
Pb ≤ 5.0 ppm
Sb ≤ 5.0 ppm
Zn ≤ 5.0 ppm

MES 一水合物缓冲液
应用
用于配制筛网或优化的结晶级 MES 一水合物
特征
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，< 5 ppb 总有机碳，无细菌（<1 细菌（CFU/ml）），无热原（<0.03 内毒素（EU/ml）） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)
描述
MES一水合物同义词：MES 或 2-（N-吗啉代）乙磺酸或 4-吗啉乙磺酸一水合物
C6H13NO4S • H2O
213.25 先生
CAS号 [145224-94-8]
EC 编号 224-632-3
贝尔斯坦注册号 329824236
RTECS QE3479500
MDL 编号 MFCD00149409
PubChem 物质 ID 24885996
纯度≥ 99.0%
25°C 时 pKa 6.1

溶液在 2-8°C 下可稳定数月。不建议对任何磺酸缓冲液进行高压灭菌。当 MES 溶液经过高压灭菌时，它们会变黄（尽管 pH 值不会发生可测量的变化）。黄色分解产物的身份未知。当 MES 溶液在室温下储存时，它们会在六个月内变黄。

MES 5.2 – 7.1 的有用 pH 值范围

使用 NaOH 将 HR2-587 滴定至所需的 pH
HR2-587 测量的 pH 值范围：3.2 – 3.4 at 25°C
HR2-587 测量电导率范围：166.4 – 2440 µS/cm at 25°C
HR2-587 测量的折射率范围：1.34655 – 1.34731 at 20°C

HR2-787 已使用 NaOH 滴定至 pH 6.5
HR2-787 测量的电导率范围：19.2 – 25.6 mS/cm at 25°C
HR2-787 测量的折射率范围：1.36113 – 1.36251 at 20°C

HR2-943-07 – MES 一水合物 pH 5.8 – 1.0 M 溶液是 Custom Shop 试剂，用于复制和优化与 Silver Bullets 试剂盒一起使用的结晶试剂。

杂质：
内毒素≤ 50 EU/g
酵母和霉菌 ≤ 100 CFU/g
生物负载 ≤ 100 CFU/g
铝 ≤ 5.0 ppm
作为 ≤ 3.0 ppm
钡 ≤ 5.0 ppm
铋 ≤ 5.0 ppm
钙 ≤ 20.0 ppm
镉 ≤ 5.0 ppm
钴 ≤ 5.0 ppm
铬 ≤ 5.0 ppm
铜 ≤ 5.0 ppm
铁 ≤ 5.0 ppm
锂 ≤ 5.0 ppm
镁 ≤ 5.0 ppm
锰 ≤ 5.0 ppm
钼 ≤ 5.0 ppm
钠 ≤ 20.0 ppm
镍 ≤ 5.0 ppm
铅 ≤ 5.0 ppm
锑 ≤ 5.0 ppm
锌 ≤ 5.0 ppm

 

0.5 M MES monohydrate

1.0 M MES monohydrate pH 6.5

 HR2-587 MES monohydrate 0.5 M solution SDS HR2-787 MES monohydrate pH 6.5 1.0 M solution SDS Titration Table

1. Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. Biochemistry 33, 1555- 1564, 1994.