Tris Buffer-HamptonTris缓冲液

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Tris Buffer-HamptonTris缓冲液
Tris Buffer-HamptonTris缓冲液

Tris Buffer-HamptonTris缓冲液

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Applications

  • Crystallization grade Tris for formulating screens or for optimization

Features

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

Tris缓冲液
应用
用于配制筛网或优化的结晶级 Tris
特征
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，< 5 ppb 总有机碳，无细菌（<1 细菌（CFU/ml）），无热原（<0.03 内毒素（EU/ml）） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)
描述
特里斯

同义词：Trizma 碱或 2-氨基-2-（羟甲基）-1,3-丙二醇或 THAM 或 Tris 碱或 Tris（羟甲基）氨基甲烷或氨基丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
EC 编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTCS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
25°C 时 pKa = 8.1

Tris 的有用 pH 范围：7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH
HR2-589 测量的 pH 值范围：10.9 – 11.1 at 25°C
HR2-589 测得的电导率范围：25°C 时为 193.3 – 236.7 µS/cm
HR2-589 测得的折射率：1.34991 – 1.35058 at 20°C

HR2-725 使用 HCl 滴定至 pH 8.5
HR2-725 测得的电导率范围：18.1 – 22.7 mS/cm at 25°C
HR2-725 测得的折射率：1.35310 – 1.35369 at 20°C

不溶物 0.005% max
未检测到 DNase
未检测到 RNase
未检测到蛋白酶
重金属 最大 5 ppm
作为 ≤ 5 ppm 最大值
Ca ≤ 5 ppm 最大值
Cu ≤ 5 ppm 最大值
Fe ≤ 5 ppm 最大值
铅≤ 5 ppm 最大值
Mg ≤ 5 ppm 最大值

Tris

Tris Buffer-HamptonTris缓冲液

 

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$55.00

cart quote

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$55.00

cart quote

Support Material(s)

Tris Buffer-HamptonTris缓冲液 HR2-589 Tris SDSTris Buffer-HamptonTris缓冲液 HR2-725 Tris pH 8.5 SDSTris Buffer-HamptonTris缓冲液 Titration Table

Certificate Of Analysis

Related Item(S)

  • Hydrochloric acid
  • StockOptions Tris Buffer Kit
  • Individual StockOptions Tris Reagents

References

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.