PEG/Ion 400-Hampton PEG/离子 400

PEG/Ion 400-Hampton PEG/离子 400

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PEG/Ion 400-Hampton PEG/离子 400

PEG/Ion 400-Hampton PEG/离子 400

Products > Crystallization Screens > PEG/Ion 400 > PEG/Ion 400

PEG/Ion 400

Applications

  • Crystallization screen for membrane and soluble biological macromolecules.

Features

  • Reagent formulation developed at Hampton Research
  • Screens a profile of anions, cations, multivalent ions, and neutralized organic acids at varying pH levels in the presence of Polyethylene glycol 400
  • pH range 5 – 9
  • Buffer free formulation
  • Compatible with lipidic cubic phase (LCP), vapor diffusion, microbatch, and free interface diffusion

Description

PEG/Ion 400 is a crystallization reagent kit designed to provide a rapid screening method for the crystallization of membrane and soluble biological macromolecules.

Most G protein-coupled receptors (GPCRs) have been crystallized in Polyethylene glycol (PEG) 400 based reagents1. PEG/Ion 400 is designed as a 96 reagent crystallization screen that is compatible with the Lipidic Cubic Phase (LCP).

PEG/Ion 400 is supplied in a sterile, polypropylene 96 Deep Well block, each reservoir containing 1 ml of sterile filtered reagent. The block is compatible with robotic and multi-channel pipet liquid handling systems and is heat sealed using a special polypropylene backed film. Each PEG/Ion 400 is supplied with an adhesive sealing film which can be used to seal the block after removing the heat seal.

The screen combines a single concentration (30% v/v) of high purity Polyethylene glycol 400 and 48 different high purity salts, comprising both anions (malate, malonate, nitrate, phosphate, succinate, sulfate, tartrate, and thiocyanate) and cations (ammonium, cadmium, calcium, ethylammonium, lithium, magnesium, nickel, potassium, sodium, tetraethylammonium, and zinc) in two concentrations (0.1 and 0.4 M) which due to their unique pH characteristics also affords a reasonable pH screen (approximate pH range of 4 to 9.5). The primary screen variables are PEG, ion type, ionic strength, salt concentration, and pH.

应用
膜和可溶性生物大分子的结晶筛选。
特征
Hampton Research 开发的试剂配方
在聚乙二醇 400 存在下筛选不同 pH 水平的阴离子、阳离子、多价离子和中和的有机酸
pH 值范围 5 – 9
无缓冲配方
与脂质立方相 (LCP)、蒸汽扩散、微批次和自由界面扩散兼容
描述
PEG/Ion 400 是一种结晶试剂盒,旨在为膜和可溶性生物大分子的结晶提供一种快速筛选方法。

大多数 G 蛋白偶联受体 (GPCR) 已在基于聚乙二醇 (PEG) 400 的试剂中结晶。 PEG/Ion 400 设计为与脂质立方相 (LCP) 兼容的 96 试剂结晶筛。

PEG/Ion 400 以无菌聚丙烯 96 Deep Well 模块形式提供,每个储液器含有 1 ml 无菌过滤试剂。该模块与机器人和多通道移液管液体处理系统兼容,并使用特殊的聚丙烯背衬薄膜进行热密封。每个 PEG/Ion 400 都配有粘性密封膜,可用于在去除热封后密封块。

该筛网结合了单一浓度 (30% v/v) 的高纯度聚乙二醇 400 和 48 种不同的高纯度盐,包括阴离子(苹果酸盐、丙二酸盐、硝酸盐、磷酸盐、琥珀酸盐、硫酸盐、酒石酸盐和硫氰酸盐)和阳离子(铵、镉、钙、乙基铵、锂、镁、镍、钾、钠、四乙基铵和锌)两种浓度(0.1 和 0.4 M),由于它们独特的 pH 特性,也提供了合理的 pH 筛选(大约 pH 范围为4 到 9.5)。主要的筛选变量是 PEG、离子类型、离子强度、盐浓度和 pH 值。

PEG/Ion 400

PEG/Ion 400-Hampton PEG/离子 400

PEG/Ion 400-Hampton PEG/离子 400
PEG/Ion 400-Hampton PEG/离子 400

CAT NO

HR2-460

NAME

PEG/Ion 400

DESCRIPTION

1 ml, Deep Well block format

PRICE

$147.00

 

Support Material(s)

PEG/Ion 400-Hampton PEG/离子 400 HR2-460 PEG/Ion 400 DocumentsPEG/Ion 400-Hampton PEG/离子 400 HR2-460 PEG/Ion 400 SDSPEG/Ion 400-Hampton PEG/离子 400 PEG/Ion 400 Formulation & Scoring Data

Related Item(S)

  • Individual PEG/Ion 400 Reagents

References

1. Successful Strategies to Determine High-Resolution Structures of GPCRs. Xiang J, Chun E, Liu C, Jing L, Al-Sahouri Z, Zhu L, Liu W. Trends Pharmacol Sci. 2016 Dec;37(12):1055-1069.

2. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. Kang Y, Zhou XE, Gao X, et al. Nature. 2015;523(7562):561-567. doi:10.1038/nature14656.

3. Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases. Fei Xu, Wei Liu, Michael A. Hanson, Raymond C. Stevens, and Vadim Cherezov. Cryst. Growth Des., 2011, 11 (4), pp 1193-1201.

4. A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Martin Caffrey. Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 3-18.