Oxoid TSA培养基 Oxoid CM0131B 胰蛋白胨大豆琼脂中文说明书

Oxoid TSA培养基 Oxoid CM0131B 胰蛋白胨大豆琼脂中文说明书

Oxoid TSA培养基 Oxoid CM0131B 胰蛋白胨大豆琼脂 TRYPTONE SOYA AGAR

Dehydrated Culture Media

TRYPTONE SOYA AGAR (Casein soya bean digest agar) EP/USP/JP/BP

脱水培养基

TRYPTONE SOYA AGAR(酪蛋白大豆消化琼脂)EP/USP/JP/BP

Code: CM0131

a general purpose medium for the growth of a wide variety of organisms

用于多种生物生长的通用培养基

Formula

gm/litre

Pancreatic digest of casein

15.0

Enzymatic* digest of soya bean

5.0

Sodium chloride

5.0

Agar

15.0

pH 7.3 ± 0.2 @ 25°C

*Contains papain


Directions
Add 40g to 1 litre of distilled water (purified as required). Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Description
A general purpose agar medium, containing two peptones, which will support the growth of a wide variety of organisms. It is suitable for the cultivation both of aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. The medium may also be used as a blood agar base – for this purpose 7% of sterile blood should be added to the sterile molten medium which has been cooled to approximately 45°C. Tryptone Soya Agar can also be used for the preparation of `chocolate’ agar.

Since Tryptone Soya Agar contains no added carbohydrate it may be used, with added blood, in the determination of haemolysis. Horse blood agar plates prepared with Tryptone Soya Agar are used for the colicine typing of Shigella sonnei 1,2,3,4.

The Oxoid medium has also been used as a replacement for yeastrel-milk agar plates in the Lisboa test 5 and for bacterial counts on eviscerated poultry6.

When supplemented with 0.7g lecithin and 5g Polysorbate (Tween 80) per litre of Tryptone Soya Agar, the medium can be used as Microbial Content Test Agar for testing quaternary ammonium compounds7.

Tryptone Soya Agar is recommended as a reference medium when testing selective media, to measure the degree of inhibition8.

A medium for isolation of Bacteroides gracilis is prepared from Tryptone Soya Agar by adding formate, fumarate and nitrate. The medium is made selective using nalidixic acid and teicoplanin9.

Enhanced haemolysis agar (EHA) used to improve detection of Listeria monocytogenes when present amongst other listeriae has been modified to optimise its performance by substituting Tryptone Soya Agar for Columbia Agar in the original formulation10. Tryptone Soya Agar conforms to formulations detailed in various international pharmacopoeia11,12,13,14.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Light straw to straw coloured gel

方向
将 40g 添加到 1 升蒸馏水中(根据需要进行纯化)。煮沸以完全溶解。通过在 121°C 高压灭菌 15 分钟进行灭菌。

描述
一种通用琼脂培养基,含有两种蛋白胨,可支持多种生物体的生长。它适用于需氧菌和厌氧菌的培养,后者可以在深层培养中生长,也可以在厌氧条件下培养。该培养基也可用作血琼脂基质——为此,应将 7% 的无菌血液添加到已冷却至约 45°C 的无菌熔融培养基中。胰蛋白胨大豆琼脂也可用于制备“巧克力”琼脂。

由于胰蛋白胨大豆琼脂不含添加碳水化合物,因此可以在添加血液的情况下用于溶血测定。用胰蛋白胨大豆琼脂制备的马血琼脂平板用于分型志贺氏志贺氏菌 1、2、3、4。

在 Lisboa 测试 5 中,Oxoid 培养基也被用作酵母菌牛奶琼脂板的替代品,以及去内脏家禽的细菌计数 6。

当每升胰蛋白胨大豆琼脂添加 0.7g 卵磷脂和 5g 聚山梨醇酯(吐温 80)时,该培养基可用作微生物含量测试琼脂,用于测试季铵化合物7。

在测试选择性培养基时,建议将胰蛋白胨大豆琼脂作为参考培养基,以测量抑制程度8。

通过添加甲酸盐、延胡索酸盐和硝酸盐,从胰蛋白胨大豆琼脂中制备用于分离纤细拟杆菌的培养基。使用萘啶酸和替考拉宁9 使培养基具有选择性。

增强型溶血琼脂 (EHA) 用于改进对单核细胞增生李斯特菌的检测(当存在于其他李斯特菌中时),已通过在原始配方中用胰蛋白胨大豆琼脂代替哥伦比亚琼脂来优化其性能10。胰蛋白胨大豆琼脂符合各种国际药典 11、12、13、14 中详述的配方。

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的培养基板储存在 2-8°C。

外貌
脱水介质:稻草色、自由流动的粉末
制备培养基:浅稻草色至稻草色凝胶

Quality control  – non-pharmaceutical testing

Positive controls: Expected results
Staphylococcus aureus ATCC® 25923 * Good growth; straw coloured colonies
Streptococcus pyogenes ATCC® 19615 * Good growth; pale straw coloured colonies
Negative control:
Uninoculated medium No change
* This organism is available as a Culti-Loop™

Quality control – pharmaceutical testing

Micro-organism Preparation of Strain Growth promotion and suitability of the counting method in presence of product
Staphylococcus aureus ATCC® 6538* TSA (casein soya bean digest agar) at 30-35°C for 18-24 hours <100 CFU in TSA (casein soya bean digest agar) at 30-35°C for ≤3 days
Pseudomonas aeruginosa ATCC® 9027*
Bacillus subtilis ATCC® 6633*
Candida albicans ATCC® 10231* Sabouraud Dextrose Agar (R454462) or Sabouraud
Dextrose Liquid Medium (CM0147) at 20-25°C for 2-3 days
<100 CFU in TSA (casein soya bean digest agar) at 30-35°C for ≤5 days
Aspergillus brasiliensis ATCC® 16404* Sabouraud Dextrose Agar (R454462) or Potato Dextrose Agar(CM0139) at 20-25°C for 5-7 days, or until good sporulation is achieved
*     This organism is available in Quanti-Cult™ format

Precautions
It should be noted that haemolytic reactions of streptococci on Tryptone Soya Agar can vary according to the origin of the blood e.g. horse or sheep. Tryptone Soya Agar designed for sheep blood show significant differences when used with horse blood and vice versa.

References
1. Abbott J. D. and Graham J. M. (1961) Mon. Bull. Min. Hlth Pub. Hlth Lab. Serv. 20. 51-58.
2. Barrow G. I. and Ellis C. (1962) Mon. Bull. Min. Hlth Pub. Hlth Lab. Serv. 21. 141-147.
3. Cooke G. T. and Daines C. F. (1964) Mon. Bull. Min. Hlth Publ. Hlth Lab. Serv. 23. 81-85.
4. Gillies R. R. (1964) J. Hyg. Camb. 62. 1-9.
5. Mitchell T. G. (1964) J. Appl. Bact. 27. 45-52.
6. Barnes Ella M. and Shrimpton D. H. (1958) J. Appl. Bact. 2. 313-329.
7. American Public Health Association (1978) Standard Methods for the Examination of Dairy Products. 14th Edn. APHA Inc. Washington DC.
8. Anon. (1987) J. Food Microbiol. 5. 291-296.
9. Lee K., Baron E.J., Summanen P. and Finegold S. (1990) J. Clin. Microbiol. 28. 1747-1750.
10. Beumer R.R., te Giffel M.C. and Cox L.J. (1997) Lett. Appl. Microbiol. 24. 421-425.
11. British Pharmacopoeia Volume II (2000)
12. US Pharmacopoeia XXX, (2008)
13. European Pharmacopoeia. 6.1 Edition (2008)
14. Japanese Pharmacopoeia. 15th Edition. (2006)

货号:名称;

CM0309B:WL营养琼脂;CM0131B:胰蛋白胨大豆琼脂TRYPTONE SOYA AGAR;

SR0222C:溶液;CM0331B:哥伦比亚血琼脂基础;CM0655B:军团CYE琼脂基础;

CM0189B:Todd-Hewitt肉汤;CM0059B:麦芽提取物琼脂(麦芽汁琼脂);CM0021B:牛奶琼脂;

BR0052G:林格氏液;CM1180B:甘露醇盐琼脂(原货号是CM0085B);

CM0119B:鲍特 菌检测用培养基;CM0469B:XLD培养基;CM1168B:营养肉汤(原货号CM0001B);

CM1135B:脑心浸出液肉汤;CM1136B:脑心浸出液琼脂;CM1177B:麦康凯肉汤 (原货号CM0005B)

LP0185B:Proteose Peptone 示蛋白胨;LP0137B:蛋白胨Bacteriological Peptone (旧货号LP0037B);

CM0129B:胰蛋白胨大豆肉汤TRYPTONE SOYA BROTH;

LP0028A:PURIFIED AGAR 琼脂糖;LP0011B:细 琼脂AGAR BACTERIOLOGICAL;

LP0047B:胰蛋白示 Tryptose;LP0044C:中性大豆蛋白胨;

LP0042B:胰蛋白胨Tryptone;CM0337B:MH琼脂;CM0107B:结晶紫中性红胆盐(乳糖)琼脂;

CM0041B:沙保氏葡萄糖琼脂;CM0325B:平板计数琼脂;LP0021B:酵母粉Yeast Extract;

CM0139B:马铃薯葡萄糖琼脂;CM1163B:MRS琼脂(原货号CM0361);

CM1175B:MRS肉汤 (原货号CM0359B);CM1174B:Nutrient Broth 2号营养肉汤(原货号CM0067B);

CM0982B:氯化钠蛋白胨缓冲液

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