NUTRIENT AGAR

Code: CM0003 (Powder)
Code: CM0004 (Tablets)

A general purpose medium which may be enriched with up to 10% blood or other biological fluid.

一种通用培养基，可富含高达 10% 的血液或其他生物液体。

* Adjusted as required to meet performance standards.

Directions
Powder: Suspend 28g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Tablets: Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.

In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms¹.

Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.

For a medium which is richer in nutrients, see Blood Agar Base No.2 CM0271.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel

方向
粉末：将 28 克悬浮在 1 升蒸馏水中。煮沸以完全溶解。通过在 121°C 高压灭菌 15 分钟进行灭菌。
片剂：将一片片加入 5ml 蒸馏水中浸泡 5 分钟。通过在 121°C 高压灭菌 15 分钟进行灭菌。

描述
营养琼脂是一种基本培养基，用于继代培养生物体以进行维护或在生化或血清学测试之前检查分离板的继代培养物的纯度。

在半固体形式、琼脂斜面或琼脂残端，培养基用于维持对照生物体1。

营养琼脂适用于教学和演示目的。它包含浓度为 1.5% 的琼脂，可根据需要添加高达 10% 的血液或其他生物液体。该培养基无需添加，可用于培养对营养要求不严格的生物。

有关营养丰富的培养基，请参见血琼脂培养基 No.2 CM0271。

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的培养基储存在 2-8°C。

外貌
脱水介质：稻草色、自由流动的粉末
制备培养基：稻草色凝胶

Quality control

* This organism is available as a Culti-Loop®

Reference
1. Lapage S. P., Shelton J. E. and Mitchell T. G. (1970)` in Methods in Microbiology’ Eds. Norris J. R. and Ribbons D. W. Vol.3A. Academic Press. London. p.116.