Oxoid CM1008T 耐苯唑西林筛选琼脂基础 O.R.S.A.B. 5KG

Oxoid CM1008T 耐苯唑西林筛选琼脂基础 O.R.S.A.B. 5KG

OXACILLIN RESISTANCE SCREENING AGAR BASE

CODE: CM1008

A medium for screening for methicillin resistant Staphylococcus aureus (MRSA) directly from routine swab samples.

一种直接从常规拭子样本中筛选耐甲氧西林金黄色葡萄球菌 (MRSA) 的培养基。

Typical Formula*

gm/litre

Peptone

11.8

Yeast Extract

9.0

Mannitol

10.0

Sodium chloride

55.0

Lithium chloride

5.0

Aniline Blue

0.2

Agar

12.5

PH 7.2 ± 0.2 @ 25°C

* Adjusted as required to meet performance standards

ORSAB SELECTIVE SUPPLEMENT

Code: SR0195

Vial contents (each vial is sufficient for 500ml of medium)
per vial
per litre
Polymyxin B

25,000IU

50,000IU
Oxacillin

1.0mg

2.0mg

Directions
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Description
ORSAB is intended as a medium for the screening for methicillin resistant Staphylococcus aureus (MRSA) directly from routine swab samples. The screening of patients and staff for the early detection of MRSA colonisation is essential if epidemics are to be prevented. ORSAB is based on traditional Mannitol Salt Agar CM0085 with a reduction in salt concentration from 75g/l (7.5%) to 55g/l (5.5%). This lower level of salt is still sufficient to inhibit most bacteria other than staphylococci whilst optimising growth of low numbers of MRSA.

Oxacillin Resistance Screening Agar Base is a nutritious and selective medium containing peptones for growth, a high salt concentration and lithium chloride to suppress non-staphylococcal growth with mannitol and aniline blue for the detection of mannitol fermentation.

The antibiotics contained in ORSAB Selective Supplement SR0195 are oxacillin at 2 mg/litre to inhibit methicillin sensitive Staphylococcus aureus (MSSA) and polymyxin B for the suppression of other bacteria able to grow at such a high salt concentration, e.g. Proteus spp.

Typical colonies of MRSA are intense blue in colour on a colourless background enabling the organism to be more easily identified in mixed culture than the pale yellow colonies seen on Mannitol Salt Agar.

描述
ORSAB 旨在用作直接从常规拭子样本中筛选耐甲氧西林金黄色葡萄球菌 (MRSA) 的介质。如果要预防流行病,对患者和工作人员进行筛查以及早发现 MRSA 定植是必不可少的。 ORSAB 基于传统的甘露醇盐琼脂 CM0085,盐浓度从 75g/l (7.5%) 降低到 55g/l (5.5%)。这种较低水平的盐仍然足以抑制除葡萄球菌以外的大多数细菌,同时优化少量 MRSA 的生长。

Oxacillin Resistance Screening Agar Base 是一种营养丰富的选择性培养基,含有用于生长的蛋白胨、高盐浓度和用于抑制非葡萄球菌生长的氯化锂,以及用于检测甘露醇发酵的甘露醇和苯胺蓝。

ORSAB 选择性补充剂 SR0195 中包含的抗生素是 2 毫克/升的苯唑西林,用于抑制对甲氧西林敏感的金黄色葡萄球菌 (MSSA) 和用于抑制其他能够在如此高盐浓度下生长的细菌的多粘菌素 B,例如变形杆菌属

MRSA 的典型菌落在无色背景上呈深蓝色,与在甘露醇盐琼脂上看到的淡黄色菌落相比,在混合培养中更容易识别微生物。

Culture Medium

Colony Colour

Positive

Negative

ORSAB
CM1008 & SR0195

Intense blue on colourless media

Straw / No Growth

Mannitol Salt Agar CM0085

Pale yellow on red media

Pink-Red / No Growth

Technique
Take a routine swab sample from the patient or person to be screened. Rub the swab onto an ORSAB plate in one set of streaks near the plate perimeter. The sample material should then be streaked across the plate using the diminishing sweep technique. Incubate at 37°C for 24 hours.
Examine after 24 hours for blue colonies. Confirm suspected MRSA with coagulase test Staphytect Plus DR0850 or Dryspot Staphytect Plus DR0100 and PBP2’ DR0900. Re-incubate negative plates for a further 24 hours if necessary. Do not re-incubate positive plates.

技术
从患者或要筛查的人身上采集常规拭子样本。 将拭子在靠近板周边的一组条纹中擦到 ORSAB 板上。 然后应使用递减扫描技术将样品材料划过板。 在 37°C 下孵育 24 小时。
24 小时后检查蓝色菌落。 用凝固酶测试 Staphytect Plus DR0850 或 Dryspot Staphytect Plus DR0100 和 PBP2’ DR0900 确认疑似 MRSA。 如有必要,将负板再孵育 24 小时。 不要重新孵育阳性板。

Storage conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
ORSAB Selective Supplement should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
ORSAB 选择性补充剂应储存在 2-8°C。
将准备好的培养基储存在 2-8°C。

Appearance
Dehydrated medium: Straw-grey coloured, free-flowing powder
Prepared medium: Blue-grey coloured gel

Quality control

Positive control: Expected results
Staphylococcus aureus (MRSA strain) ATCC® 43300 * Good growth; blue coloured colonies
Negative controls:
Staphylococcus aureus (MSSA strain) ATCC® 25923 * No growth
Escherichia coli ATCC® 25922 * No growth
* This organism is available as a Culti-Loop®

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