Oxoid CM0972A 厌氧基础琼脂 ANAEROBE BASAL AGAR 100G

Oxoid CM0972A 厌氧基础琼脂 ANAEROBE BASAL AGAR 100G

Dehydrated Culture Media

ANAEROBE BASAL AGAR

Code: CM0972

A nutrient agar for the growth of anaerobic micro-organisms, particularly Bacteroides spp. and other fastidious anaerobes.

一种用于厌氧微生物生长的营养琼脂,特别是拟杆菌属。 和其他挑剔的厌氧菌。

Typical Formula*

gm/litre

Peptone

16.0

Yeast extract

7.0

Sodium chloride

5.0

Starch

1.0

Dextrose

1.0

Sodium pyruvate

1.0

Arginine

1.0

Sodium succinate

0.5

L-cysteine HCl

0.25

Sodium bicarbonate

0.4

Ferric pyrophosphate

0.5

Haemin

0.005

Vitamin K

0.0005

Dithiothreitol

0.25

Agar

12.0

pH 6.8 ± 0.2 @ 25°C

* Adjusted as required to meet performance standards

Directions
Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.

Mix well and pour into sterile Petri dishes.

Description
Anaerobe Basal Agar contains peptones, carefully selected to support good growth of anaerobic bacteria and yeast extract as a vitamin source. Starch is present to absorb any toxic metabolites1. Sufficient arginine is added to ensure the growth of Eubacterium lentum2, whilst haemin and vitamin K are growth factors required by many Bacteroides species3. Haemin is also required by Porphyromonas species. Sodium succinate improves the growth of Prevotella melaninogenica and Bacteroides species4. Sodium pyruvate is added as an energy source for asaccharolytic cocci such as Veillonella. It also acts similarly to catalase and degrades traces of hydrogen peroxide, which may be produced by the action of molecular oxygen on media components5. L-cysteine hydrochloride and dithiothreitol are reducing agents, and cysteine has also been shown to stimulate the growth of some anaerobes6.

描述
厌氧菌基础琼脂含有经过精心挑选的蛋白胨,以支持厌氧菌的良好生长,酵母提取物作为维生素来源。 存在淀粉以吸收任何有毒代谢物1。 添加足够的精氨酸以确保慢速真杆菌的生长2,而血红素和维生素 K 是许多拟杆菌属物种所需的生长因子 3。 卟啉单胞菌也需要血红素。 琥珀酸钠可促进黑色素普氏菌和拟杆菌的生长4。 添加丙酮酸钠作为解糖球菌如韦荣氏菌的能量来源。 它的作用也类似于过氧化氢酶并降解微量的过氧化氢,过氧化氢可能是由分子氧对培养基成分的作用产生的5。 L-半胱氨酸盐酸盐和二硫苏糖醇是还原剂,半胱氨酸也被证明可以刺激一些厌氧菌的生长。

Technique
Inoculate the medium by surface plating to obtain single colonies. Incubate anaerobically for up to 5 days at 37°C. Anaerobic conditions can be achieved using the Oxoid AnaeroGen Atmosphere Generation System AN0025 with the Oxoid AnaeroJar AG0025.

The medium may be rendered selective for Gram-negative anaerobes by the addition of G-N supplement SR0108 and for non-sporing anaerobes by the addition of N-S supplement SR0107 with Tween 80. Neomycin Selective Supplement SR0163 can be added to select for Clostridia.

技术
通过表面电镀接种培养基以获得单菌落。 在 37°C 下厌氧培养长达 5 天。 厌氧条件可以使用 Oxoid AnaeroGen 大气生成系统 AN0025 和 Oxoid AnaeroJar AG0025 来实现。

通过添加 G-N 补充剂 SR0108 可以使培养基对革兰氏阴性厌氧菌具有选择性,通过添加 N-S 补充剂 SR0107 和 Tween 80 可以使培养基对非孢子厌氧菌具有选择性。可以添加新霉素选择性补充剂 SR0163 以选择梭菌。

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium for upto 3 weeks at 2-8°C.

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的培养基板在 2-8°C 下储存长达 3 周。

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel

外貌
脱水介质:稻草色、自由流动的粉末
制备培养基:稻草色凝胶

Quality control

Positive controls: Expected results
Peptostreptococcus anaerobius ATCC® 27337 * Good growth; grey coloured colonies
Prevotella melaninogenica ATCC® 25845 * Good growth; grey coloured colonies
Clostridium perfringens ATCC® 13124 Good growth; grey coloured colonies
Negative control:
Uninoculated medium No change
* This organism is available as a Culti-Loop®

References 参考文献
1. Ajello GW. Geely JC. Hayes PS. et al. Trans-isolate medium: a new medium for primary culturing and transport of Neisseria meningitidisStreptococcus pneumoniae and Haemophilus influenzae. J. Clin. Micro. 1984:20:55-8.
2. Sperry JF. Wilkins TD. Arginine, a growth-limiting factor for Eubacterium lentum. J. Bacteriol 1976:127:780-4.
3. Gibbons RJ. and MacDonnald JB. Haemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J. Bact. 1960:80:164-170.
4. Lev M. Keudell KC. and Milford AF. Succinate as a growth factor for Bacteroides melaninogenicus. J. Bact. 1971:108:175-8.
5. Neilson PA. Role of reduced sulphur compounds in nutrition of Proprionobacterium acnes. J. Clin. Micr. 1983:17:276-9.
6. Shanson DC. and Singh J. Effect of adding cysteine to brain-heart infusion broth on the isolation of Bacteroides fragilis from experimental blood cultures. J. Clin. Path. 1981:34:221-3.

发表回复