Oxoid CM0965B 弓形杆菌肉汤 ARCOBACTER BROTH 500G
品牌:Oxoid
用于弓形杆菌的选择性增菌
弓形杆菌肉汤基础:CM0965B
CAT添加剂:SR0174E,SR0174H
CCDA添加剂:SR0155E,SR0155H
弓形杆菌(Arcobacter)属于弯曲菌科,革兰氏阴性,与弯曲菌(Campylobacter)类似。但不同的是,它在需氧、微需氧和15℃ 低温条件下均可生长。Arcobacter 是一种人畜共患的食源性和水源性病原菌,主要分离自猪肉、牛肉、禽肉等动物性食品和水体。
弓形杆菌肉汤 (Arcobacter Broth)
弓形杆菌属的选择性增菌肉汤,使用肉汤基础CM0965B加添加剂SR0174或SR0155,这两种添加剂在弯曲杆菌分离培养基中也涉及到。
货号 | 产品描述 | 规格 | 可配制培养基 | 公开价 |
CM0965B | 弓形杆菌肉汤基础 Arcobacter Broth Base |
500g | 20.8L | 1796元 |
SR0174E | CAT添加剂 CAT Supplement |
10小瓶 | 每小瓶500ml | 1484元 |
SR0174H | 10小瓶 | 每小瓶2.0L | 5570元 | |
SR0155E | CCDA选择性添加剂 CCDA Selective Supplement |
10小瓶 | 每小瓶500ml | 792元 |
SR0155H | 10小瓶 | 每小瓶2.0L | 4310元 | |
SR0155N | 10小瓶 | 每小瓶9.0L | 18529元 |
弓形杆菌肉汤基础 CM0965:
配方表:
成分* | 含量 g/L |
蛋白胨 | 18.0 |
酵母粉 | 1.0 |
氯化钠 | 5.0 |
pH 7.2±0.2 @25℃ |
*可按照标准需要自行调整配方
CCDA选择性添加剂 SR0155:
成分 | SR0155E每小瓶含量 | 每升培养基含量 |
头孢哌酮 | 16mg | 32mg |
两性霉素B | 5mg | 10mg |
CAT选择性添加剂 SR0174:
成分 | SR0174E每小瓶含量 | 每升培养基含量 |
头孢哌酮 | 4.0mg | 8.0mg |
替考拉宁 | 2.0mg | 4.0mg |
两性霉素B | 5.0mg | 10.0mg |
使用方法:
称取12g的弓形杆菌肉汤基础到500ml蒸馏水中,121℃灭菌15分钟,冷却到50℃。添加1小瓶SR0155E或者SR0174E配成CAT肉汤,分装到无菌培养瓶中。
使用CAT添加剂可以选择性增菌弓形杆菌属, 而使用CCDA添加剂的选择性跟强,可以选择性增菌Arcobacter butzleri布氏弓形杆菌。
Dehydrated Culture Media
ARCOBACTER BROTH
Code: CM0965
An enrichment broth for Arcobacter species
Arcobacter 菌种的富集肉汤
Typical Formula* |
gm/litre |
Peptone |
18.0 |
Yeast extract |
1.0 |
Sodium chloride |
5.0 |
pH 7.2 ± 0.2 @ 25°C |
|
* Adjusted as required to meet performance standards
Directions
Dissolve 12g of Arcobacter Broth in 500ml of distilled water. Sterilise at 121°C for 15 minutes and allow to cool to below 50°C
For use with CAT supplement (SR0174)
Add one vial per 500ml medium of CAT Selective Supplement reconstituted as directed. Dispense into sterile containers.
For use with CCDA supplement (SR0155)
Add one vial per 500ml medium of CCDA Selective Supplement reconstituted as directed. Dispense into sterile containers.
Inoculated plates should be incubated aerobically, at 30°C, for 24 hours.
Description
Oxoid Arcobacter Broth is intended for use with Cefoperazone, Amphotericin B, Teicoplanin (CAT) Selective Supplement (SR0174) as a selective enrichment broth for the growth of Arcobacter species and with the more selective CCDA (SR0155) for the selective enrichment of Arcobacter butzleri.
Peptones in the base medium are specifically designed to provide the ideal growth conditions for Arcobacter species. The incubation conditions and the absence of blood or charcoal supplements suppress the growth of Campylobacter species. Cefoperazone, Amphotericin B and Teicoplanin are added to suppress the growth of competing flora, but allow the growth of Arcobacter species. CCDA Selective Supplement SR0155 is substituted for CAT to selectively isolate Arcobacter butzleri1,2.
Arcobacters are micro aerophilic, Gram-negative rods, which were formerly classified as Campylobacter3. Four Arcobacter species have been identified: A. butzleri, A. cryaerophilus, A. skirrowii and A. nitrofigilis, all of which have a greater propensity to grow in air than Campylobacter spp.
Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii have been associated with disease in humans4,5, and typically are isolated from faecal samples.
Arcobacter butzleri has been isolated from patients with bacteraemia, peritonitis, endocarditis and diarrhoea. Patients with Arcobacter butzleri-associated diarrhoea typically suffer from abdominal pain and nausea, fever, chills, vomitting and malaise, but the organism has also been implicated in an outbreak of recurrent abdominal cramps without diarrhoea6. The source of infection is usually contaminated water or sewage6.
Arcobacter cryaerophilus group 1B has been isolated from patients with bacteraemia and diarrhoea4,5, although it is a much less common human isolate than Arcobacter butzleri6.
Arcobacter nitrofigilis has only been isolated from marsh grass to date, never from humans or animals. It is not thought to be clinically significant7.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Quality control
Positive control: | Expected Results |
Arcobacter butzleri ATCC® 49616 | Colourless colonies |
Negative control: | |
Escherichia coli ATCC® 25922 | Inhibited |
References
1. Data on file
2. Lammerding, A.M., Harris, J.E., Lior, D.L. et al. Presented at the 81st annual meeting of IAMFES (1994).
3. Vandamme, P., Falsen, E., Rossau, R., Hoste, B., Segers, P., Tytgat, R., De Ley, J. (1991). Int. J. Syst. Bacteriol. 41:88-103.
4. Kiehlbauch, J.A., Brenner, D.J., Nicholson, M.A., Baker, C.N., Patton, C.M., Steigerwalt, A.G., Wachsmuth, I.K. (1991). J. Clin. Microbiol. 29:376-385.
5. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van den Borre, C., Higgins, R., Hommer, J. (1992). Int. J. Syst. Bacteriol. 42:344-356.
6. Vandamme, P., Pugina, P., Benzi, G., Van Etterick, R., Vlaes, L., Kersters, K., Butzler, J., Lior, H., Lauwers, S. (1992). J. Clin. Microbiol. 30:2335-2337.
7. Atabay, H.I and Corry, J.E.L. (1998) Int. J. Food Microbiol, 41, 53-58.