Oxoid CM0935B Karmali弯曲杆菌琼脂基础 KARMALI AGAR BASE 500G

Oxoid CM0935B Karmali弯曲杆菌琼脂基础 KARMALI AGAR BASE 500G

Dehydrated Culture Media

CAMPYLOBACTER AGAR BASE (KARMALI)

Code: CM0935

A blood free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.

一种无血选择性培养基,用于在 42°C 孵育时分离空肠弯曲杆菌和大肠杆菌。

Typical Formula*

gm/litre

Columbia Agar Base

39.0

Activated charcoal

4.0

Haemin

0.032

Final pH 7.4 ± 0.2 @ 25°C
* Adjusted as required to meet performance standards

CAMPYLOBACTER SELECTIVE SUPPLEMENT (KARMALI)

弯曲杆菌选择性补充剂(KARMALI)

Code: SR0167

Vial contents:(each vial is sufficient for 500ml of medium)
per vial
per litre
Sodium pyruvate

50.0mg

100.0mg

Cefoperazone

16.0mg

32.0mg

Vancomycin

10.0mg

20.0mg

Cycloheximide

50.0mg

100.0mg

MODIFIED KARMALI SELECTIVE SUPPLEMENT

Code: SR0205

Vial contents: (each vial is sufficient for 500ml of medium)
per vial
per litre
Sodium pyruvate

50.0mg

100.0mg

Cefoperazone

16.0mg

32.0mg

Vancomycin

10.0mg

20.0mg

Amphotericin B

5.0mg

10.0mg

Directions
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.

方向
将 21.5 克弯曲杆菌琼脂培养基 (Karmali) 添加到 500 毫升蒸馏水中并煮沸溶解。 通过在 121°C 高压灭菌 15 分钟进行灭菌。 冷却至 50°C。 无菌添加 1 瓶弯曲杆菌选择性补充剂 (Karmali) SR0167 或改良的 Karmali 选择性补充剂 SR0205,按照指示重新配制。充分混合并倒入无菌培养皿中。

Description
Campylobacter Medium (Karmali) is based on the formulation described by Karmali et al.1 and is recommended for the isolation of Campylobacter jejuni and Campylobacter coli from clinical specimens.

The original Campylobacter Blood Free medium in the Oxoid product range contains sodium pyruvate in the agar base. Campylobacter Medium (Karmali) incorporates this ingredient into the selective supplement. The original medium also contains sodium desoxycholate for the inhibition of Gram positive organisms, whereas with Campylobacter Medium (Karmali) suppression of Gram positives is achieved by the inclusion of vancomycin. Modified Karmali Selective Supplement SR0205 offers a cycloheximide free alternative by utilising amphotericin B as an antifungal agent3.

Campylobacter jejuni strains produce grey, moist, flat spreading colonies after 42 hour incubation at 42°C.

If plates are first examined after 24 hours incubation, read them immediately and quickly return them to a reduced oxygen atmosphere to ensure continued viability of the more oxygen-sensitive strains.

At 42°C selectivity is increased and growth is faster but non-thermophilic strains will not grow e.g. Campylobacter fetus subsp. fetus.
Colonies tend to swarm when initially isolated from clinical specimens.

描述
弯曲杆菌培养基 (Karmali) 基于 Karmali 等人 1 描述的配方,推荐用于从临床标本中分离空肠弯曲杆菌和大肠杆菌。

Oxoid 产品系列中的原始无血弯曲杆菌培养基在琼脂基质中含有丙酮酸钠。弯曲杆菌培养基 (Karmali) 将这种成分加入到选择性补充剂中。原始培养基还含有用于抑制革兰氏阳性菌的脱氧胆酸钠,而弯曲杆菌培养基 (Karmali) 则通过加入万古霉素来抑制革兰氏阳性菌。改良的 Karmali 选择性补充剂 SR0205 通过利用两性霉素 B 作为抗真菌剂 3 提供了一种不含放线菌酮的替代品。

空肠弯曲杆菌菌株在 42°C 温育 42 小时后会产生灰色、潮湿、平展的菌落。

如果在培养 24 小时后首次检查板,请立即阅读它们并迅速将它们放回低氧环境中,以确保对氧更敏感的菌株继续存活。

在 42°C 时选择性增加,生长更快,但非嗜热菌株不会生长,例如胎儿弯曲杆菌亚种。胎儿。
当最初从临床标本中分离出来时,菌落往往会成群结队。

Technique
1. Prepare Campylobacter Selective Medium (Karmali) plates as described in the directions for use.
2. Emulsify approximately 0.5g of the specimen in 5ml of sterile 0.1% peptone water to form a 1:10 dilution.
3. Inoculate on to selective medium with cotton tipped swabs so that single isolated colonies are formed.
4. Incubate the plates in an atmosphere consisting of approximately 5-6% oxygen, 10% carbon dioxide and 84-85% nitrogen for 48 hours at 42°C. This can best be achieved by using the Oxoid Gas Generating Kit for Campylobacters BR0056 in conjunction with the Oxoid Anaerobic Jar and an active catalyst BR0042. For jars of smaller capacity (2.5 litres) use the Oxoid Gas Generating Kit for Campylobacters BR0060. Alternatively use CampyGen CN0025 or CN0035. CampyGen does not require the addition of water or a catalyst.
5. Examine the plates and confirm the typical colonies as Campylobacter jejuni or Campylobacter coli. A simple schema for differentiating Campylobacter species is described by Skirrow and Benjamin2.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

储存条件和保质期
弯曲杆菌琼脂培养基 (Karmali) 应密封保存在原始容器中,置于阴凉干燥处,远离强光。 在 10-30°C 下储存时,介质将保持稳定,直至标签上印制的有效期。

Appearance
Dehydrated medium: Black, free-flowing powder
Prepared medium: Black gel

外貌
脱水介质:黑色、自由流动的粉末
制备培养基:黑色凝胶

Quality control

Positive control: Expected results
Campylobacter jejuni ATCC® 33291 * Good growth; grey coloured colonies
Negative control:
Escherichia coli ATCC® 25922 * Inhibited
* This organism is available as a Culti-Loop®

Precautions 参考文献
Campylobacter Selective Supplement (Karmali) SR0167 contains cycloheximide which is toxic.
Note the precautions to be taken under HAZARDOUS  Products section of the Manual.

References
1. Karmali M.A., Simor A.E., Roscoe M., Fleming P.C, Smith S.S. and Lane J. (1986) J.Clin.Micro. 23. 456-459.
2. Skirrow M.B. and Benjamin J. (1980) J.Clin.Path. 33.1122.
3. Data on file at Oxoid.

发表回复