Oxoid CM0898B HTM基础培养基 HAEMOPHILUS TESTING MEDIUM
货号:CM0898B
规格:500g
品牌:Oxoid
Dehydrated Culture Media
HAEMOPHILUS TEST MEDIUM HTM BASE
Code: CM0898
A medium specifically formulated for the susceptibility testing of Haemophilus influenzae.
一种专门用于流感嗜血杆菌敏感性测试的培养基。
Typical Formula* |
gm/litre |
Mueller-Hinton Agar |
38.0 |
Yeast extract (specifically selected for low antagonist levels) |
5.0 |
pH 7.3 ± 0.1 @ 25°C |
* Adjusted as required to meet performance standards
HTM SUPPLEMENT
Code: SR0158
Vial contents (each vial is sufficient for 500ml of medium) |
per vial
|
per litre
|
NAD |
7.5mg |
15.0mg
|
Haematin |
7.5mg |
15.0mg
|
Directions
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.
方向
将 21.5g 嗜血杆菌测试培养基基质悬浮在 500ml 蒸馏水中。 煮沸溶解。 通过在 121°C 高压灭菌 15 分钟进行灭菌。 冷却至 50°C 并按照指示无菌添加 1 瓶嗜血杆菌测试培养基补充剂 SR0158 的内容物。 充分混合并倒入无菌培养皿中。
Description
Haemophilus Test Medium (HTM) has been specifically formulated for the susceptibility testing of Haemophilus influenzae. The medium forms part of the recommended methods of the United States National Committee for Clinical Laboratory Standards (NCCLS)1.
Haemophilus influenzae require complex media for growth. These complex media have aggravated the routine susceptibility testing of Haemophilus influenzae because of antagonism between some essential nutrients and certain antimicrobial agents. Difficulties in interpreting inhibition zones may also arise.
Oxoid Haemophilus Test Medium (HTM) is based on the formulation developed by Jorgensen et al.2 which is now recommended by the United States NCCLS. The results achieved using HTM have been found to be highly reproducible3,4. Comparisons with Mueller-Hinton Chocolate Agar have shown an overall agreement of 99.6%5.
The transparency of the medium allows zones of inhibition to be read easily through the bottom of the Petri dish. HTM contains low levels of antimicrobial antagonists, which allows testing of trimethoprim/sulphamethoxazole to be carried out with greater confidence.
Technique
1 Prepare the inoculum in Mueller-Hinton Broth CM0405 or 0.9% saline, to match the turbidity of 0.5 McFarland standard.
2 Using a swab saturated with the above inoculum suspension, inoculate the surface of a Haemophilus Test Medium Agar plate to give confluent growth.
3 Apply the antimicrobial discs on to the surface of the Haemophilus Test Medium plate.
4 Incubate the plates at 35°C in 5-7% carbon dioxide for 16-18 hours and measure the zones of inhibition.
NB: In the absence of a CO2 incubator, the CO2 enriched atmosphere can best be achieved by using the Oxoid Gas Generating Kit BR0039 in conjunction with Oxoid Anaerobic Jar or alternatively use CO2Gen CD0025.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
HTM Supplement as supplied should be stored in the dark below 0°C. When stored as directed the supplement is stable until the expiry date stated on the label.
Appearance
Dehydrated medium: Light straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
Quality control
Positive control: | Expected results |
Haemophilus influenzae ATCC® 49766 * | Good growth; straw coloured colonies |
Negative control: | |
Uninoculated medium. | No change |
* This organism is available as a Culti-Loop®
References
1. NCCLS Documents M2-A7 Vol. 20. No.1. and M7-A5 Vol. 20. No 2.
2. Jorgensen J.H., Redding J.S., Maher L.A. and Howell A.W. (1987) J. Clin. Micro. 25, 2105-2113.
3. Doern G.V., Jorgensen J.H., Thornsberry C. and Snapper H. (1990). Eur. J. Clin. Microbiol. Infect. Dis. 9, 329-336.
4. Barry A.L., Jorgensen J.H. and Hardy D.J. (1991) J. Antimic. Chem. 27, 295-301.
5. Evans G., Marsik F., Thompson L. and Fowler J. (1990) Abstracts of ASM Meeting 1990 C-252.
产品名称及用途 | 规格 | 说明 | 货号 |
诊断敏感性试验琼脂(D.S.T.A.) | 500g | 可以配制12.5升培养基 | CM0261B |
Diagnostic Sensitivity Test Agar (D.S.T.A.) | 5kg | 可以配制125.0升培养基 | CM0261T |
嗜血杆菌检测培养基(HTM) Haemophilus Test Medium (HTM) | |||
用于流感嗜血杆菌的抗生素敏感检测 | |||
HTM基础 HTM Base | 500g | 可以配制11.6升培养基 | CM0898B |
HTM添加剂 HTM Supplement(储存于-20℃-0℃) | 10小瓶 | 可以配制500毫升培养基 | SR0158E |
Iso标准药敏检测琼脂 | 500g | 可以配制15.9升培养基 | CM0471B |
Iso-Sensitest Agar | 2.5kg | 可以配制79.5升培养基 | CM0471R |
用于抗生素敏感试验的半合成培养基 | 5kg | 可以配制159.0升培养基 | CM0471T |
Iso标准药敏检测肉汤 | 500g | 可以配制21.4升培养基 | CM0473B |
Iso-Sensitest Broth | |||
用于抗生素敏感试验的半合成培养基 | |||
MH琼脂(CLSI标准) | 500g | 可以配制13.2升培养基 | CM0337B |
Mueller-Hinton Agar CLSI | 2.5kg | 可以配制66.0升培养基 | CM0337R |
与国际标准抗生素敏感检测培养基一致,符合CLSIM6-A2规定 | 5kg | 可以配制132.0升培养基 | CM0337T |
MH肉汤(CLSI标准) | 500g | 可以配制23.8升培养基 | CM0405B |
Mueller-Hinton Broth CLSI | |||
与国际标准抗生素敏感检测培养基一致 | |||
药敏检测琼脂 | 500g | 可以配制15.6升培养基 | CM0409B |
Sensitest Agar | 2.5kg | 可以配制78.0升培养基 | CM0409R |
Wilkins-Chalgren 厌氧琼脂 | 500g | 可以配制11.6升培养基 | CM0619B |
Wilkins-Chalgren Anaerobe Agar | |||
推荐用于厌氧菌的药敏试验 | |||
Wilkins-Chalgren 厌氧肉汤 | 500g | 可以配制15.2升培养基 | CM0643B |
Wilkins-Chalgren Anaerobe Broth | |||
推荐用于厌氧菌的药敏试验 | |||
一号抗生素培养基(种子琼脂) | 500g | 可以配制18.5升培养基 | CM0327B |
Antibiotic Medium No.1(Seed agar) | |||
推荐作为种子层用于制备抗生素的微生物检测平板 | |||
三号抗生素培养基(检测肉汤)USP | 500g | 可以配制28.6升培养基 | CM0287B |
Antibiotic Medium No.3(Assay broth) | |||
用于青霉素和其它抗生素的连续稀释法测定 |