Oxoid CM0898B HTM基础培养基 HAEMOPHILUS TESTING MEDIUM

Oxoid CM0898B HTM基础培养基 HAEMOPHILUS TESTING MEDIUM

货号:CM0898B
规格:500g
品牌:Oxoid

Dehydrated Culture Media

HAEMOPHILUS TEST MEDIUM HTM BASE

Code: CM0898

A medium specifically formulated for the susceptibility testing of Haemophilus influenzae.

一种专门用于流感嗜血杆菌敏感性测试的培养基。

Typical Formula*

gm/litre

Mueller-Hinton Agar

38.0

Yeast extract (specifically selected for low antagonist levels)

5.0

pH 7.3 ± 0.1 @ 25°C
* Adjusted as required to meet performance standards

HTM SUPPLEMENT

Code: SR0158

Vial contents (each vial is sufficient for 500ml of medium)
per vial
per litre
NAD

7.5mg

15.0mg
Haematin

7.5mg

15.0mg

Directions
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

方向
将 21.5g 嗜血杆菌测试培养基基质悬浮在 500ml 蒸馏水中。 煮沸溶解。 通过在 121°C 高压灭菌 15 分钟进行灭菌。 冷却至 50°C 并按照指示无菌添加 1 瓶嗜血杆菌测试培养基补充剂 SR0158 的内容物。 充分混合并倒入无菌培养皿中。

Description
Haemophilus Test Medium (HTM) has been specifically formulated for the susceptibility testing of Haemophilus influenzae. The medium forms part of the recommended methods of the United States National Committee for Clinical Laboratory Standards (NCCLS)1.

Haemophilus influenzae require complex media for growth. These complex media have aggravated the routine susceptibility testing of Haemophilus influenzae because of antagonism between some essential nutrients and certain antimicrobial agents. Difficulties in interpreting inhibition zones may also arise.

Oxoid Haemophilus Test Medium (HTM) is based on the formulation developed by Jorgensen et al.2 which is now recommended by the United States NCCLS. The results achieved using HTM have been found to be highly reproducible3,4. Comparisons with Mueller-Hinton Chocolate Agar have shown an overall agreement of 99.6%5.

The transparency of the medium allows zones of inhibition to be read easily through the bottom of the Petri dish. HTM contains low levels of antimicrobial antagonists, which allows testing of trimethoprim/sulphamethoxazole to be carried out with greater confidence.

Technique
1
 Prepare the inoculum in Mueller-Hinton Broth CM0405 or 0.9% saline, to match the turbidity of 0.5 McFarland standard.
2 Using a swab saturated with the above inoculum suspension, inoculate the surface of a Haemophilus Test Medium Agar plate to give confluent growth.
3 Apply the antimicrobial discs on to the surface of the Haemophilus Test Medium plate.
4 Incubate the plates at 35°C in 5-7% carbon dioxide for 16-18 hours and measure the zones of inhibition.
NB: In the absence of a CO2 incubator, the CO2 enriched atmosphere can best be achieved by using the Oxoid Gas Generating Kit BR0039 in conjunction with Oxoid Anaerobic Jar or alternatively use CO2Gen CD0025.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
HTM Supplement as supplied should be stored in the dark below 0°C. When stored as directed the supplement is stable until the expiry date stated on the label.

Appearance
Dehydrated medium: Light straw coloured, free-flowing powder
Prepared medium: Straw coloured gel

Quality control

Positive control: Expected results
Haemophilus influenzae ATCC® 49766 * Good growth; straw coloured colonies
Negative control:
Uninoculated medium. No change
* This organism is available as a Culti-Loop®

References
1. NCCLS Documents M2-A7 Vol. 20. No.1. and M7-A5 Vol. 20. No 2.
2. Jorgensen J.H., Redding J.S., Maher L.A. and Howell A.W. (1987) J. Clin. Micro. 25, 2105-2113.
3. Doern G.V., Jorgensen J.H., Thornsberry C. and Snapper H. (1990). Eur. J. Clin. Microbiol. Infect. Dis. 9, 329-336.
4. Barry A.L., Jorgensen J.H. and Hardy D.J. (1991) J. Antimic. Chem. 27, 295-301.
5. Evans G., Marsik F., Thompson L. and Fowler J. (1990) Abstracts of ASM Meeting 1990 C-252.

产品名称及用途 规格 说明 货号
诊断敏感性试验琼脂(D.S.T.A.) 500g 可以配制12.5升培养基 CM0261B
Diagnostic Sensitivity Test Agar (D.S.T.A.)  5kg 可以配制125.0升培养基   CM0261T  
   
嗜血杆菌检测培养基(HTM)  Haemophilus Test Medium (HTM)    
用于流感嗜血杆菌的抗生素敏感检测  
HTM基础 HTM Base  500g 可以配制11.6升培养基 CM0898B
HTM添加剂 HTM Supplement(储存于-20-0℃)  10小瓶 可以配制500毫升培养基 SR0158E 
   
Iso标准药敏检测琼脂  500g  可以配制15.9升培养基 CM0471B
Iso-Sensitest Agar  2.5kg 可以配制79.5升培养基 CM0471R
用于抗生素敏感试验的半合成培养基 5kg    可以配制159.0升培养基 CM0471T
   
Iso标准药敏检测肉汤 500g 可以配制21.4升培养基  CM0473B 
Iso-Sensitest Broth  
用于抗生素敏感试验的半合成培养基      
   
MH琼脂(CLSI标准)   500g  可以配制13.2升培养基 CM0337B
Mueller-Hinton Agar CLSI 2.5kg   可以配制66.0升培养基  CM0337R 
与国际标准抗生素敏感检测培养基一致,符合CLSIM6-A2规定 5kg  可以配制132.0升培养基 CM0337T
   
MH肉汤(CLSI标准)   500g  可以配制23.8升培养基   CM0405B  
Mueller-Hinton Broth CLSI    
与国际标准抗生素敏感检测培养基一致      
   
药敏检测琼脂 500g     可以配制15.6升培养基 CM0409B
Sensitest Agar  2.5kg  可以配制78.0升培养基  CM0409R 
   
Wilkins-Chalgren 厌氧琼脂 500g  可以配制11.6升培养基 CM0619B 
Wilkins-Chalgren Anaerobe Agar   
推荐用于厌氧菌的药敏试验       
   
Wilkins-Chalgren 厌氧肉汤 500g  可以配制15.2升培养基 CM0643B 
Wilkins-Chalgren Anaerobe Broth   
推荐用于厌氧菌的药敏试验       
   
一号抗生素培养基(种子琼脂)  500g 可以配制18.5升培养基 CM0327B
Antibiotic Medium No.1(Seed agar)  
推荐作为种子层用于制备抗生素的微生物检测平板      
   
三号抗生素培养基(检测肉汤)USP 500g 可以配制28.6升培养基 CM0287B
Antibiotic Medium No.3(Assay broth)  
用于青霉素和其它抗生素的连续稀释法测定

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