Oxoid CM0854B 绵羊血琼脂培养基 SHEEP BLOOD AGAR
Dehydrated Culture Media
BLOOD AGAR BASE (SHEEP)
Code: CM0854
A Blood Agar Base that has been specifically formulated to give improved haemolytic reactions with sheep blood.
一种血琼脂基质,经过专门配制,可改善羊血的溶血反应。
Typical Formula* |
gm/litre |
Tryptone |
14.0 |
Peptone Neutralised |
4.5 |
Yeast extract |
4.5 |
Sodium chloride |
5.0 |
Agar |
12.0 |
Final pH 7.3 ± 0.2 @ 25°C |
|
* Adjusted as required to meet performance standards
Directions
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 7% sterile sheep blood.
Description
Sheep Blood Agar Base was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of organisms without interfering with their haemolytic reactions when used with sheep blood. Sheep Blood Agar Base is based on the formulation of Blood Agar Base No.2 CM0271.
Blood Agar Base No.2 when supplemented with sheep blood was occasionally found to result in a mixed haemolytic reaction (alpha and beta haemolysis) for some Group A Streptococci (Streptococcus pyogenes). These mixed haemolytic reactions were due to trace amounts of fermentable carbohydrates in yeast extract and the physiological differences of sheep blood when compared to horse blood1.
Having identified the causes of the mixed haemolytic reactions, the Sheep Blood Agar Base was formulated to be compatible with sheep blood. Comparisons with other blood agar bases supplemented with sheep blood have shown that with Sheep Blood Agar Base the growth of many bacteria – especially the fastidious streptococci – is considerably improved, and the expected beta haemolytic reaction is achieved with Streptococcus pyogenes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Appearance
Dehydrated medium: Straw-coloured, free-flowing powder
Prepared medium: Straw-coloured gel
方向
将 40g 悬浮在 1 升蒸馏水中并煮沸以完全溶解。通过在 121°C 高压灭菌 15 分钟进行灭菌。冷却至 50°C 并在无菌条件下加入 7% 无菌羊血。
描述
羊血琼脂基质的开发是为了满足对特别有营养的血琼脂基质的需求,当与羊血一起使用时,这种基质可以最大限度地恢复生物体,而不会干扰它们的溶血反应。羊血琼脂基础是基于血琼脂基础 No.2 CM0271 的配方。
偶尔发现添加羊血的血琼脂基础 2 会导致某些 A 组链球菌(化脓性链球菌)发生混合溶血反应(α 和 β 溶血)。这些混合溶血反应是由于酵母提取物中微量的可发酵碳水化合物以及羊血与马血相比的生理差异1。
确定了混合溶血反应的原因后,羊血琼脂基质被配制为与羊血相容。与添加了羊血的其他血琼脂基质进行比较表明,使用绵羊血琼脂基质可显着改善许多细菌(尤其是挑剔的链球菌)的生长,并通过化脓性链球菌实现预期的 β 溶血反应。
储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的培养基储存在 2-8°C。
外貌
脱水介质:稻草色、自由流动的粉末
制备培养基:稻草色凝胶
Quality control
Positive controls: | Expected results |
Streptococcus pyogenes ATCC® 19615 * | Good growth; colourless/white colonies |
Streptococcus pneumoniae ATCC® 6303 * | Good growth; colourless colonies |
Negative control: | |
Uninoculated medium | No change |
* This organism is available as a Culti-Loop®
Reference
1. Spector W S (1961) Handbook of Biological Data, P51 and 53. In WS Spector (Ed.) Handbook of Biological Data. W B Saunder Company, Philadelphia and London.
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