Oxoid现货 CM0793B tergitol-7琼脂基础 TERGITOL-7-AGAR (MODIFIED)

TERGITOL-7 AGAR

Code: CM0793

A selective medium for the detection and enumeration of coliforms.

用于检测和计数大肠菌群的选择性培养基。

Typical Formula*	gm/litre
Peptone	10.0
Yeast extract	6.0
Meat extract	5.0
Lactose	20.0
Bromothymol blue	0.05
Tergitol-7	0.1
Agar	13.0
pH 7.2 ± 0.2 @ 25°C

* Adjusted as required to meet performance standards

TTC Solution (SR0148) is supplied as 2ml of filtered 0.125% aqueous solution of tri-phenyltetrazolium chloride (TTC).

Directions
Suspend 54.15g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense in 100ml volumes and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the contents of 1 vial of SR0148. Mix well and pour into sterile Petri dishes.

Description
Tergitol-7 Agar is a selective and differential medium for the detection and enumeration of coliforms in food and water samples.

Tergitol-7 Agar is based on the formulation described by Chapman¹ and is recommended for the selective isolation and differentiation of the coliform group. The use of Tergitol-7 as a selective agent had been described earlier².

The addition of tri-phenyltetrazolium chloride (TTC)³ allows earlier recognition and identification of Escherichia coli and Enterobacter aerogenes. This medium has been recommended for examining foodstuffs for faecal contamination⁴ and has been successfully used in routine water analysis⁵.

Tergitol-7 inhibits Gram positive organisms and minimises the swarming of Proteus allowing superior recovery of coliforms. Fermentation of lactose is seen by a change in colour of the pH indicator bromothymol blue. TTC is rapidly reduced to insoluble red formazan by most coliform organisms except Escherichia coli and Enterobacter aerogenes, thus allowing easy differentiation. Tergitol-7 Agar is quoted in the EU Directive for the detection and enumeration of coliforms in drinking water^6.

Technique
Inoculate by spreading the sample on the surface of the agar. Incubate at 35°C for up to 24 hours.

TTC 溶液 (SR0148) 以 2ml 过滤后的 0.125% 三苯基四唑氯化物 (TTC) 水溶液的形式提供。

方向
将 54.15 克悬浮在 1 升蒸馏水中。煮沸以完全溶解。分装成 100ml 体积，在 121°C 高压灭菌 15 分钟进行灭菌。冷却至 50°C 并添加 1 瓶 SR0148 的内容物。充分混合并倒入无菌培养皿中。

描述
Tergitol-7 Agar 是一种选择性和差异培养基，用于检测和计数食品和水样中的大肠菌群。

Tergitol-7 Agar 基于 Chapman1 描述的配方，推荐用于大肠菌群的选择性分离和分化。使用 Tergitol-7 作为选择剂已在前面进行了描述2。

添加氯化三苯基四唑 (TTC)3 可以更早地识别和鉴定大肠杆菌和产气肠杆菌。该介质已被推荐用于检查食品的粪便污染4，并已成功用于常规水分析5。

Tergitol-7 抑制革兰氏阳性菌并最大限度地减少变形杆菌的聚集，从而实现大肠菌群的卓越回收。通过 pH 指示剂溴百里酚蓝的颜色变化可以看出乳糖的发酵。除大肠杆菌和产气肠杆菌外，大多数大肠菌群都会将 TTC 迅速还原为不溶性红色甲臜，因此易于分化。欧盟指令中引用了 Tergitol-7 琼脂，用于检测和计数饮用水中的大肠菌群6。

技术
通过将样品铺在琼脂表面上进行接种。在 35°C 下孵育长达 24 小时。

Escherichia coli	Yellow colonies with yellow zone Sometimes with rust coloured centre
Enterobacter/Klebsiella species	Greenish/yellow colonies
Salmonella species	Red colony with bluish zone
Shigella species	Red colony with bluish zone
Proteus species	Red colony with bluish zone
Pseudomonas species	Red colony with bluish zone
Gram positive bacteria	No growth to slight growth

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Pale green coloured, free-flowing powder
Prepared medium: Dark green coloured gel

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将准备好的培养基储存在 2-8°C。

外貌
脱水介质：淡绿色、自由流动的粉末
制备培养基：深绿色凝胶

Quality control

Positive control:	Expected results
Escherichia coli ATCC® 25922 *	Good growth; yellow-orange coloured colonies with yellow zone
Negative control:
Staphylococcus aureus ATCC® 25923 *	Inhibited or no growth

* This organism is available as a Culti-Loop®

Precautions
Tergitol-7 Agar is designed for early detection of Escherichia coli i.e. 6-10 hours incubation³.
Incubation at 44°C has been recommended ^4,5.

References
1. Chapman G. H. (1947) J. Bact. 53. 504.
2. Pollard A.L. (1946) Science 103. 758-759.
3. Chapman G.H. (1951) Am. J. Pub. Hlth 41. 1381.
4. Mossel D. A. A. (1962) J. Appl. Bact. 25. 20-29.
5. Kulp W., Mascoli C., Tavshanjian O. (1953) Am. J. Pub. Hlth 43. 1111-1113.
6. E.U. (1998) 98/83/EC of Council of 3rd of November 1998 on the quality of water intended for human consumption. Off. J. Eur. Commun., L3330, 32-54.

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