Oxoid代理 SR0222C OXOID 0.1%放线酮溶液使用说明

Oxoid代理 SR0222C OXOID 0.1%放线酮溶液使用说明

Culture Media Supplements

CYCLOHEXIMIDE 0.1% SOLUTION

CODE: SR0222

A sterile cycloheximide solution for the addition to a range of Oxoid media for the suppression of fungi.

Description
Cycloheximide 0.1% Solution (SR0222), supplied in a glass bottle with screw-cap, is safer and easier to use than cycloheximide powder. The required volume can be simply aliquoted from the bottle (stored in the dark at 2-8ºC) and added to the medium before or after sterilisation.
Suitable for addition to a range of media, including the following (please refer to The Oxoid Manual or website dev.oxoid.com for details):

CM0041 SABOURAUD DEXTROSE AGAR
An acidic medium for the isolation of dermatophytes, other fungi and yeasts. Cycloheximide, chloramphenicol and various other antimicrobials have been recommended for addition to Sabouraud Dextrose Agar (CM0041). The antimicrobials chosen and levels at which they are used will depend on the group of organisms that is to be inhibited. e.g. Cryptococcus neoformans, Aspergillus fumigatus and Allescheria boydii are sensitive to cycloheximide.

CM0375 BRAIN HEART INFUSION AGAR
A solid medium which contains the highly nutritious infusions recommended for the cultivation of fastidious organisms. For the selective isolation of fungi, cycloheximide at 0.5 µg/ml (0.5 ml of SR0222/litre of medium) and chloramphenicol (SR0078) at 0.05 µg/ml may be added.

CM0309 W. L. NUTRIENT AGAR & CM0501 W. L. NUTRIENT BROTH
Two media for the determination of the microbiological flora in brewing and fermentation. Cycloheximide may be added to the medium at a final concentration of 4 µg/ml (4 ml of SR0222/litre of medium) for the selective isolation of bacterial contaminants in yeast cultures.

CM0617 + SR0099 BACILLUS CEREUS SELECTIVE AGAR (PEMBA)
A selective and diagnostic medium for the isolation and enumeration of Bacillus cereus in food and other samples. It is recommended that, where large numbers of fungi are expected in the sample, cycloheximide is added to the medium at a final concentration of 40 µg/ml (40 ml of SR0222/litre of medium).

CM0651 UNIVERSAL BEER AGAR
A medium developed for the isolation of beer spoilage organisms. The addition of cycloheximide at 1 µg/ml (1 ml of SR0222/litre of medium) to suppress yeast growth gives a medium that is selective for the detection of bacterial contaminants in yeast cultures.

CM0777 RAKA-RAY AGAR
The addition of phenylethanol and cycloheximide forms a selective medium for the isolation of lactic acid bacteria in beer and brewing processes. Selectivity is achieved by the addition of 3 g/litre of 2-phenylethanol to inhibit Gram-negative organisms and 7 µg/ml of cycloheximide (7 ml of SR0222/litre of medium) to inhibit yeasts.

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环己酰亚胺 0.1% 溶液

代码:SR0222

一种无菌的放线菌酮溶液,用于添加到一系列 Oxoid 培养基中以抑制真菌。

描述
放线菌酮 0.1% 溶液 (SR0222) 装在带螺旋盖的玻璃瓶中,比放线菌酮粉末更安全、更易于使用。所需体积可以简单地从瓶子中分装(储存在 2-8ºC 的黑暗中)并在灭菌之前或之后添加到培养基中。
适合添加到一系列媒体中,包括以下内容(请参阅 The Oxoid 手册或网站 dev.oxoid.com 了解详细信息):

CM0041 SABOURAUD 葡萄糖琼脂
用于分离皮肤癣菌、其他真菌和酵母菌的酸性介质。已推荐将环己酰亚胺、氯霉素和各种其他抗菌剂添加到沙氏葡萄糖琼脂 (CM0041) 中。选择的抗微生物剂和它们的使用水平将取决于要抑制的生物群。例如新型隐球菌、烟曲霉和 Allescheria boydii 对放线菌酮敏感。

CM0375 脑心灌注琼脂
一种固体培养基,含有推荐用于培养挑剔生物的高营养输液。对于真菌的选择性分离,可以添加 0.5 µg/ml 的放线菌酮(0.5 ml SR0222/升培养基)和 0.05 µg/ml 的氯霉素 (SR0078)。

CM0309 W. L. 营养琼脂 & CM0501 W. L. 营养肉汤
用于测定酿造和发酵中微生物菌群的两种介质。放线菌酮可以以 4 µg/ml 的终浓度(4 ml SR0222/升培养基)添加到培养基中,用于选择性分离酵母培养物中的细菌污染物。

CM0617 + SR0099 蜡状芽孢杆菌选择性琼脂 (PEMBA)
用于分离和计数食品和其他样品中蜡状芽孢杆菌的选择性和诊断培养基。建议在样品中预期有大量真菌的情况下,将放线菌酮以 40 µg/ml 的最终浓度添加到培养基中(40 ml SR0222/升培养基)。

CM0651 通用啤酒琼脂
一种用于分离啤酒腐败菌的培养基。添加 1 µg/ml 的放线菌酮(1 ml SR0222/升培养基)以抑制酵母菌生长,提供了一种对检测酵母培养物中的细菌污染物具有选择性的培养基。

CM0777 RAKA-RAY 琼脂
苯乙醇和放线菌酮的加入形成了一种选择性培养基,用于啤酒和酿造过程中乳酸菌的分离。通过添加 3 g/l 的 2-苯基乙醇来抑制革兰氏阴性菌和 7 µg/ml 的放线菌酮(7 ml SR0222/l 培养基)来抑制酵母菌,可以实现选择性。

OXOID CM1002B 念珠菌显色琼脂培养基 500g/瓶
OXOID CM1005B 鼠李糖山梨醇麦康凯培养基 500g/瓶
OXOID CM1007B 沙门氏菌显色琼脂培养基 500g/瓶
OXOID CM1008B 耐苯唑西林筛选琼脂培养基 500g/瓶
OXOID CM1012B 水平板计数琼脂培养基 500g/瓶
OXOID CM1028B/CM1193B 碱性蛋白胨水 500g/瓶
OXOID CM1031B 滤膜法乳糖葡萄糖苷酸培养基 500g/瓶
OXOID CM1032B 心浸出液肉汤 500g/瓶
OXOID CM1036B 蜡样芽孢杆菌显色琼脂培养基 500g/瓶
OXOID CM1046B 大肠杆菌/大肠菌群显色琼脂 500g/瓶
OXOID CM1049B 缓冲蛋白胨水(ISO) 500g/瓶
OXOID CM1055B 阪崎肠杆菌显色琼脂培养基 500g/瓶

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