Oxoid CM1135B 脑心浸出液肉汤(Brain Heart Infusion Broth)成分表

Oxoid CM1135B 脑心浸出液肉汤(Brain Heart Infusion Broth)成分表

BHI肉汤
脑心浸出液肉汤(Brain Heart Infusion Broth)
货号:CM1135B
规格:500g

BRAIN HEART INFUSION BROTH

Code: CM1135

A highly nutritious infusion medium recommended for the cultivation of streptococci, Neisseria and other fastidious organisms.

Formula

gm/litre

Brain infusion solids

12.5

Beef heart infusion solids

5.0

Proteose peptone

10.0

Glucose

2.0

Sodium chloride

5.0

Disodium phosphate

2.5

pH 7.4 ± 0.2 @ 25°C

Directions
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

方向
将 37g 溶解在 1 升蒸馏水中。 充分混合并分配到最终容器中。 通过在 121°C 高压灭菌 15 分钟进行灭菌。

Description
A versatile liquid infusion medium which is suitable for the cultivation of streptococci, Neisseria and other fastidious organisms, this medium is recommended for blood culture work and, with the additions described below, for the isolation and cultivation of pathogenic fungi.

Oxoid Brain Heart Infusion Broth is essentially a buffered infusion broth, giving similar results to the brain dextrose broths originally employed for the cultivation of streptococci1, and for the cultivation of dental pathogens2. The addition of 0.1% agar will serve to reduce convection currents and so create conditions of varying oxygen tension which favour the growth and primary isolation of aerobes and anaerobes3, while even easily cultivated organisms show improved growth4.

Oxoid Brain Heart Infusion Broth was used in a test for the pathogenicity of streptococci5,6 and the same medium was enriched with ascitic fluid for the cultivation of gonococci7. It is especially useful as a growth and suspension medium for staphylococci which are to be tested for coagulase production; Newman8 employed a similar medium for this purpose in an investigation of food poisoning caused by dairy products.

A satisfactory medium for blood culture can be prepared by adding 1g of agar per litre of Brain Heart Infusion Broth. Ensure that the agar is uniformly distributed in the sterile broth before dispensing into bottles.

Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.

Further supplements to improve the recovery of organisms from blood can be added before sterilization or aseptically post-sterilization. Co-enzyme1 (NAD), penicillinase and p-amino benzoic acid are examples. Brain Heart Infusion Broth supplemented with yeast extract, haemin and menadione was consistently better in producing heavy growth of five species of Bacteroides than three standard anaerobic broths. Furthermore, microscopy of overnight cultures showed normal morphology in Brain Heart Infusion Broth but abnormal morphology in the three anaerobic broths9.

描述
一种多功能液体输液培养基,适用于培养链球菌、奈瑟氏球菌和其他苛求微生物,推荐用于血培养工作,并添加下述添加剂,用于病原真菌的分离和培养。

Oxoid Brain Heart Infusion Broth 本质上是一种缓冲的输液肉汤,其结果与最初用于培养链球菌1 和培养牙科病原体2 的脑葡萄糖肉汤相似。添加 0.1% 的琼脂将有助于减少对流,从而创造不同的氧张力条件,有利于需氧菌和厌氧菌的生长和初级隔离3,而即使是容易培养的生物也显示出改善的生长4。

Oxoid Brain Heart Infusion Broth 用于测试链球菌 5、6 的致病性,相同的培养基中富含用于培养淋球菌 7 的腹水。它特别适合作为葡萄球菌的生长和悬浮培养基,用于检测凝固酶的产生; Newman8 为此目的使用了类似的介质来调查乳制品引起的食物中毒。

每升脑心浸液肉汤中加入 1g 琼脂即可制备出令人满意的血培养培养基。确保琼脂在分配到瓶子之前均匀分布在无菌肉汤中。

未在灭菌当天使用的Oxoid Brain Heart Infusion Broth管应置于沸水浴中几分钟以去除吸收的氧气,并在使用前快速冷却而不摇晃。

可以在灭菌前或灭菌后添加其他补充剂以提高血液中微生物的回收率。辅酶 1 (NAD)、青霉素酶和对氨基苯甲酸是例子。与三种标准厌氧肉汤相比,添加酵母提取物、血红素和甲萘醌的脑心浸液肉汤在产生五种拟杆菌的大量生长方面始终更好。此外,过夜培养物的显微镜显示脑心浸液肉汤形态正常,但三种厌氧肉汤形态异常9。

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.

储存条件和保质期
将脱水培养基储存在 10-30°C 并在标签上的有效期之前使用。
将管装或瓶装培养基储存在 20°C 以下的黑暗中。

Appearance
Dehydrated medium: Straw coloured, free-flowing powder.
Prepared medium: Straw coloured solution.

外貌
脱水培养基:稻草色、自由流动的粉末。
制备培养基:稻草色溶液。

Quality control

Positive controls Expected results
Streptococcus pneumoniae ATCC® 6303* Turbid growth
Candida albicans ATCC® 10231* Turbid growth
Negative control
Uninoculated medium No change

*This organism is available as a Culti-Loop®

References  参考文献:
1. Rosenow E. C. (1919) J. Dental Research l. 205-249.
2. Haden R. L. (1923) Arch. Internal Med. 32. 828-849.
3. Hitchens A. P. (1921) J. Infectious Diseases 29. 390-407.
4. Falk C. R. et al. (1939) J. Bact. 37. 121-131.
5. Chapman G. H. et al. (1944) Am. J. Clin. Path. 9: Tech. Suppl. 3. 20-26.
6. Chapman G. H. (1946) Am. J. Digestive Diseases 13. 105-107.
7. Reitzel R. J. and Kohl C. (1938) J. Am. Med. Assoc. 110. 1095-1098.
8. Newman R. W. (1950) J. Milk and Food Tech. 13. 226-233.
9. Eley A., Greenwood D. and O’Grady F

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